Production of fungal α-amylase by Saccharomyces kluyveri in glucose-limited cultivations

Journal of Biotechnology

Volumn 111, Issue 3, 2004, Pages 311-318

  Møller, Kasper ^a   Sharif, Mostafa Z ^a   Olsson, Lisbeth ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Amylase; Continuous cultivation; Fed batch cultivation; Heterologous protein production; S. kluyveri

Indexed keywords

BIOMASS; CELLS; FUNGI; GLUCOSE; PROTEINS;

FED-BATCH CULTIVATION; HETEROLOGOUS PROTEINS;

BIOTECHNOLOGY;

AMYLASE; FUNGAL ENZYME; GLUCOSE;

ARTICLE; BIOMASS; CONCENTRATION (PARAMETERS); DILUTION; DRY WEIGHT; ENZYME RELEASE; EVALUATION; FED BATCH CULTURE; GENE EXPRESSION; GROWTH RATE; NONHUMAN; PLASMID; PRIORITY JOURNAL; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; YEAST;

ALPHA-AMYLASE; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIVISION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; GLUCOSE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SACCHAROMYCES;

ASPERGILLUS; ASPERGILLUS ORYZAE; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES KLUYVERI;

EID: 3042712918     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.04.013     Document Type: Article

References (18)

1. Alexander M.A., Jeffries T.W. Respiratory efficiency and metabolite partitioning as regulatory phenomena in yeasts. Enzyme Microb. Technol. 12:1990;2-19
2. Choi B.K., Bobrowicz P., Davidson R.C., Hamilton S.R., Kung D.H., Li H., Miele R.G., Nett J.H., Wildt S., Gerngross T.U. Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris. Proc. Natl. Acad. Sci. USA. 100:2003;5022-5027
3. Gellissen G. Heterologous protein production in methylotrophic yeasts. Appl. Microbiol. Biotechnol. 54:2000;741-750
4. Gietz R.D., Schiestl R.H. Transforming yeast with DNA. Methods Mol. Cell Biol. 5:1995;255-269
5. Hamilton S.R., Bobrowicz P., Bobrowicz B., Davidson R.C., Li H., Mitchell T., Nett J.H., Rausch S., Stadheim T.A., Wischnewski H., Wildt S., Gerngross T.U. Production of complex human glycoproteins in yeast. Science. 301:2003;1244-1246
6. Hensing M.C., Rouwenhorst R.J., Heijnen J.J., van Dijken J.P., Pronk J.T. Physiological and technological aspects of large-scale heterologous-protein production with yeasts. Antonie Van Leeuwenhoek. 67:1995;261-279
7. van Hoek P., de Hulster E., van Dijken J.P., Pronk J.T. Fermentative capacity in high-cell-density fed-batch cultures of baker's yeast. Biotechnol. Bioeng. 68:2000;517-523
8. van Hoek P., Flikweert M.T., van der Aart Q.J., Steensma H.Y., van Dijken J.P., Pronk J.T. Effects of pyruvate decarboxylase overproduction on flux distribution at the pyruvate branch point in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 64:1998;2133-2140
9. Käppeli O. Regulation of carbon metabolism in Saccharomyces cerevisiae and related yeasts. Adv. Microb. Physiol. 28:1986;181-209
10. Kondo K., Miura Y., Sone H., Kobayashi K., Iijima H. High-level expression of a sweet protein, monellin, in the food yeast Candida utilis. Nat. Biotechnol. 15:1997;453-457
11. Møller K., Bro C., Piškur J., Nielsen J., Olsson L. Steady-state and transient-state analyses of aerobic fermentation in Saccharomyces kluyveri. FEMS Yeast Res. 2:2002a;233-244
12. Møller K., Christensen B., Förster J., Piškur J., Nielsen J., Olsson L. Aerobic glucose metabolism of Saccharomyces kluyveri: growth, metabolite production, and quantification of metabolic fluxes. Biotechnol. Bioeng. 77:2002b;186-193
13. Møller K., Tidemand L.D., Winther J.R., Olsson L., Piškur J., Nielsen J. Production of a heterologous proteinase A by Saccharomyces kluyveri. Appl. Microbiol. Biotechnol. 57:2001;216-219
14. Nieto A., Prieto J.A., Sanz P. Stable high-copy-number integration of Aspergillus oryzae α-amylase cDNA in an industrial baker's yeast strain. Biotechnol. Prog. 15:1999;459-466
15. Pronk J.T., Steensma H.Y., van Dijken J.P. Pyruvate metabolism in Saccharomyces cerevisiae. Yeast. 12:1996;1607-1633
16. Romanos M.A., Scorer C.A., Clare J.J. Foreign gene expression in yeast. Yeast. 8:1992;423-488
17. Sikorski R.S., Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:1989;19-27
18. Verduyn C., Postma E., Scheffers W.A., van Dijken J.P. Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast. 8:1992;501-517