Purification of a recombinant membrane protein tagged with a calmodulin-binding domain: Properties of chimeras of the Escherichia coli nicotinamide nucleotide transhydrogenase and the C-terminus of human plasma membrane Ca2+-ATPase

Protein Expression and Purification

Volumn 36, Issue 1, 2004, Pages 31-39

  Egorov, Maxim V ^a   Tigerström, Anna ^b   Pestov, Nikolay B ^a   Korneenko, Tatyana V ^a   Kostina, Maria B ^a   Shakhparonov, Mikhail I ^a   Rydström, Jan ^b  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Affinity chromatography; Calmodulin binding domain; Membrane protein; Plasma membrane Ca2+ ATPase; Transhydrogenase

Indexed keywords

ADENOSINE TRIPHOSPHATASE (CALCIUM); CALMODULIN; CALMODULIN BINDING PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; PROTEIN SUBUNIT;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CELL MEMBRANE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; CALCIUM-TRANSPORTING ATPASES; CALMODULIN; CALMODULIN-BINDING PROTEINS; CELL MEMBRANE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; NADP TRANSHYDROGENASE; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 3042633864     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.03.002     Document Type: Article

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