Site-directed mutagenesis of charged and potentially proton-carrying residues in the β subunit of the proton-translocating nicotinamide nucleotide transhydrogenase from escherichia coli. Characterization of the βH91, βD392, and βK424 mutants

Biochemistry

Volumn 38, Issue 5, 1999, Pages 1652-1658

  Hu, Xiang ^a   Zhang, Junwei ^a   Fjellström, Ola ^a   Bizouarn, Tania ^a   Rydström, Jan ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

3 ACETYLPYRIDINE; BACTERIAL ENZYME; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SODIUM CHLORIDE;

ARTICLE; BINDING AFFINITY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME SUBUNIT; ESCHERICHIA COLI; NONHUMAN; PH; PRIORITY JOURNAL; PROTON TRANSPORT; REDUCTION; SITE DIRECTED MUTAGENESIS;

ASPARTIC ACID; BIOLOGICAL TRANSPORT; CATALYSIS; CONSERVED SEQUENCE; ENZYME ACTIVATION; ESCHERICHIA COLI; GLUTAMIC ACID; HISTIDINE; HYDROGEN-ION CONCENTRATION; LYSINE; MAGNESIUM CHLORIDE; MUTAGENESIS, SITE-DIRECTED; NADP; NADP TRANSHYDROGENASE; PROTON PUMPS; RECOMBINANT PROTEINS; SODIUM CHLORIDE;

EID: 0033514291     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi981654b     Document Type: Article

References (31)

1. Meuller, J., Zhang J., Hou, C., Bragg, P. D., and Rydström, J. (1997) Biochem. J. 324, 681-687.
2. Bizouarn, T., Sazanov, L. A., Aubourg, S., and Jackson, J. B. (1996) Biochim. Biophys. Acta 1273, 4-12.
3. Jackson, J. B. (1991) J. Bioenerg. Biomembr. 23, 715-741.
4. Olausson, T., Fjellström, O., Meuller, J., and Rydström, J. (1995) Biochim. Biophys. Acta 1231, 1-19.
5. Diggle, C., Hutton, M., Jones, G. R., Thomas, C. M., and Jackson, J. B. (1995) Eur. J. Biochem. 228, 719-726.
6. Diggle, C., Cotton, N. P., Grimley, R. L., Quirk, P. G., Thomas, C. M., and Jackson, J. B. (1995) Eur. J. Biochem. 232, 315-326.
7. Fjellström, O., Johansson, C., and Rydström, J. (1997) Biochemistry 36, 11331-11341.
8. Yamaguchi, M., and Hatefi, Y. (1997) Biochim. Biophys. Acta 1318, 225-234.
9. Yamaguchi, M., and Hatefi, Y. (1995) J. Biol. Chem. 270, 28165-28168.
10. Diggle, C., Bizouarn, T., Cotton, N. P. J., and Jackson, J. B. (1996) Eur. J. Biochem. 241, 162-170.
11. Zhang, J., Hu, X., Osman, A. M., and Rydström, J. (1997) Biochim. Biophys. Acta 1319, 331-339.
12. Hutton, M., Day, J. M., Bizouarn, T., and Jackson, J. B. (1994) Eur. J. Biochem, 219, 1041-1051.
13. Holmberg, E., Olausson, T., Hultman, T., Rydström, J., Ahmad, S., Glavas, N. A., and Bragg, P. D. (1994) Biochemistry 33, 7691-7700.
14. Glavas, N. A., Hou, C., and Bragg, P. D. (1995) Biochemistry 34, 7694-7702.
15. Meuller, J., Hu, X., Bunthof, C., Olausson, T., and Rydström, J. (1996) Biochim. Biophys. Acta 1273, 191-194.
16. Fillingame, R. H. (1996) Curr. Opin. Struct. Biol. 6, 491-498.
17. Bragg, P. D., and Hou, C. (1996) Eur. J. Biochem. 241, 611-618.
18. Bragg, P. D., Glavas, N. A., and Hou, C. (1997) Arch. Biochem. Biophys. 338, 57-66.
19. Ahmad, S., Glavas, N. A., and Bragg, P. D. (1992) Eur. J. Biochem. 207, 733-739.
20. Glavas, N. A., Ahmad, S., Bragg, P. D., Olausson, T., and Rydström, J. (1993) J. Biol. Chem. 268, 14125-14130.
21. Hu, X., Zhang, J., Persson, A., and Rydström, J. (1995) Biochim. Biophys. Acta 1229, 64-72.
22. Rydström, J. (1979) Methods Enzymol. 55F, 261-275.
23. Peterson, G. L. (1977) Anal. Biochem. 83, 346-356.
24. Glavas, N. A., and Bragg, P. D. (1995) Biochim. Biophys. Acta 1231, 297-303.
25. Venning, J. D., Grimley, R. L., Bizouarn, T., Cotton, N. P. J., and Jackson, J. B. (1997) J. Biol. Chem. 272, 27535-27538.
26. Bizouarn, T., Stilwell, S., Venning, J., Cotton, N. P. J., and Jackson, J. B. (1997) Biochim. Biophys. Acta 1322, 19-32.
27. Bragg, P. D. (1996) FEBS Lett. 397, 93-96.
28. Stilwell, S. N., Bizouarn, T., and Jackson, J. B. (1997) Biochim. Biophys. Acta 1320, 83-94.
29. Sazanov, L. A., and Jackson, J. B. (1995) Biochim. Biophys. Acta 1231, 304-312.
30. O'Neal, S. G., Earle, S. G., and Fisher, R. R. (1980) Biochim. Biophys. Acta 589, 217-230.
31. Milgrom, Y. M., and Hatefi, Y. (1994) Biochem. Mol. Biol. Int. 34, 1099-1108.