Pyruvate shuttling during rest and exercise before and after endurance training in men

Journal of Applied Physiology

Volumn 97, Issue 1, 2004, Pages 317-325

  Henderson, Gregory C ^a   Horning, Michael A ^a   Lehman, Steven L ^a   Wolfel, Eugene E ^b   Bergman, Bryan C ^b   Brooks, George A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Exertion; Intermediary metabolism; Lactate shuttle; Physical activity; Tracers

Indexed keywords

ALANINE; LACTIC ACID; PYRUVIC ACID; TRACER;

ADULT; ARTERIAL BLOOD; ARTICLE; ENDURANCE; EQUILIBRIUM CONSTANT; EXERCISE; GLYCOLYSIS; HUMAN; HUMAN EXPERIMENT; ISOTOPE LABELING; MALE; METABOLISM; MUSCLE LEVEL; NORMAL HUMAN; PHYSICAL ACTIVITY; PRIORITY JOURNAL; REST; TRANSPORT KINETICS;

ADULT; ALANINE; ANAEROBIC THRESHOLD; BODY COMPOSITION; CARBOHYDRATE METABOLISM; EXERCISE; EXERCISE TEST; FEMORAL ARTERY; FEMORAL VEIN; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLYCOLYSIS; HEMODYNAMIC PROCESSES; HUMANS; LACTIC ACID; MALE; MUSCLE, SKELETAL; PHYSICAL ENDURANCE; PHYSICAL FITNESS; PYRUVIC ACID; REST;

EID: 3042558495     PISSN: 87507587     EISSN: None     Source Type: Journal    
DOI: 10.1152/japplphysiol.01367.2003     Document Type: Article

References (48)

1. Ahlborg G and Felig P. Lactate and glucose exchange across the forearm, legs, and splanchnic bed during and after prolonged leg exercise. J Clin Invest 69: 45-54, 1982.
2. Asmussen E. Blood pyruvate and ventilation in heavy work. Acta Physiol Scand 20: 133-136, 1949.
3. Asmussen E. Pyruvate and lactate content of the blood during and after muscular work. Acta Physiol Scand 20: 125-132, 1949.
4. Avogaro A, Toffolo G, Miola M, Valerio A, Tiengo A, Cobelli C, and Del Prato S. Intracellular lactate- and pyruvate-interconversion rates are increased in muscle tissue of non-insulin-dependent diabetic individuals. J Clin Invest 98: 108-115, 1996.
5. Bassett DJP and Bowen-Kelly E. Pyruvate metabolism of perfused rat lungs after exposure to 100% oxygen. J Appl Physiol 60: 1605-1609, 1986.
6. 2 exposure. Am J Physiol Lung Cell Mol Physiol 262: L495-L501, 1992.
7. Bergman BC, Butterfield GE, Wolfel EE, Casazza GA, Lopaschuk GD, and Brooks GA. An evaluation of exercise and training on muscle lipid metabolism. Am J Physiol Endocrinol Metab 276: E106-E117, 1999.
8. Bergman BC, Butterfield GE, Wolfel EE, Lopaschuk GD, Casazza GA, Horning MA, and Brooks GA. Muscle net glucose uptake and glucose kinetics after endurance training in men. Am J Physiol Endocrinol Metab 277: E81-E92, 1999.
9. Bergman BC, Horning MA, Casazza GA, Wolfel EE, Butterfield GE, and Brooks GA. Endurance training increases gluconeogenesis during rest and exercise in men. Am J Physiol Endocrinol Metab 278: E244-E251, 2000.
10. Bergman BC, Wolfel EE, Butterfield GE, Lopaschuk GD, Casazza GA, Horning MA, and Brooks GA. Active muscle and whole body lactate kinetics after endurance training in men. J Appl Physiol 87: 1684-1696, 1999.
11. Brooks GA. Current concepts in lactate exchange. Med Sci Sports Exerc 23: 895-906, 1991.
12. Brooks GA, Brown M, Butz CE, Sicurello JP, and Dubouchaud H. Cardiac and skeletal muscle mitochondria have a monocarboxylate transporter MCT1. J Appl Physiol 87: 1713-1718, 1999.
13. Brooks GA, Butterfield GE, Wolfe RR, Groves BM, Mazzeo RS, Sutton JR, Wolfel EE, and Reeves JT. Decreased reliance on lactate during exercise after acclimatization to 4,300 m. J Appl Physiol 71: 333-341, 1991.
14. Brooks GA, Dubouchaud H, Brown M, Sicurello JP, and Butz CE. Role of mitochondrial lactate dehydrogenase and lactate oxidation in the intracellular lactate shuttle. Proc Natl Acad Sci USA 96: 1129-1134, 1999.
15. Brooks GA, Wolfel EE, Groves BM, Bender PR, Butterfield GE, Cymerman A, Mazzeo RS, Sutton JR, Wolfe RR, and Reeves JT. Muscle accounts for glucose disposal but not lactate release during exercise after acclimatization to 4,300 m. J Appl Physiol 72: 2435-2445, 1992.
16. Bueding E and Goodhart R. Removal of pyruvic acid from human blood in vitro. J Biol Chem 141: 931-944, 1941.
17. Chatham JC, Des Rosiers C, and Forder JR. Evidence of separate pathways for lactate uptake and release by perfused rat heart. Am J Physiol Endocrinol Metab 281: E794-E802, 2001.
18. Chinkes DL, Zhang XI, Romijn JA, Sakurai Y, and Wolfe RR. Measurement of pyruvate and lactate kinetics across the hindlimb and gut of anesthetized dogs. Am J Physiol Endocrinol Metab 267: E174-E182, 1994.
19. Faintrenie G and Géloën A. Alpha-1 adrenergic regulation of lactate production by white adipocytes. J Pharmacol Exp Ther 277: 235-238, 1996.
20. Gertz EW, Wisneski JA, Stanley WC, and Neese RA. Myocardial substrate utilization during exercise in humans: dual carbon-labeled carbohydrate isotope experiments. J Clin Invest 82: 2017-2025, 1988.
21. Gladden LB, Crawford RE, and Webster MJ. Effect of lactate concentration and metabolic rate on net lactate uptake by canine skeletal muscle. Am J Physiol Regul Integr Comp Physiol 266: R1095-R1101, 1994.
22. 2 during work at sea level and high altitude. J Appl Physiol 23: 523-530, 1967.
23. Jackson A and Pollock M. Generalized equations for predicting body density of men. Br J Nutr 40: 497-504, 1978.
24. Jöbsis FF and Stainsby WN. Oxidation of NADH during contractions of circulated mammalian skeletal muscle. Respir Physiol 4: 292-300, 1968.
25. Johnson JA and Fusaro RM. The role of the skin in carbohydrate metabolism. Adv Metab Disord 60: 1-55, 1972.
26. Kelley KM, Hamann JJ, Navarre C, and Gladden LB. Lactate metabolism in resting and contracting canine skeletal muscle with elevated lactate concentration. J Appl Physiol 93: 865-872, 2002.
27. Large V, Soloviev M, Brunengraber H, and Beylot M. Lactate and pyruvate isotopic enrichments in plasma and tissues of postabsorptive and starved rats. Am J Physiol Endocrinol Metab 268: E880-E888, 1995.
28. Lundgren F, Bennegard K, Elander A, Lundholm K, Schersten T, and Bylund-Fellenius AC. Substrate exchange in human limb muscle during exercise at reduced blood flow. Am J Physiol Heart Circ Physiol 255: H1156-HI164, 1988.
29. 13C tracer studies of lactate metabolism in humans during rest and two levels of exercise. Biomed Mass Spectrom 9: 310-314, 1982.
30. Miller BF, Fattor JA, Jacobs KA, Horning MA, Navazio F, Lindinger MI, and Brooks GA. Lactate and glucose interactions during rest and exercise in men: effect of exogenous lactate infusion. J Physiol 544: 963-975, 2002.
31. Pfleiderer G. L-Alanine: determination with glutamate pyruvate transaminase and lactic dehydrogenase. In: Methods of Enzymatic Analysis (2nd ed.), edited by H. Bergmeyer. New York: Academic, 1963, p. 378-380.
32. Pianosi P, Seargeant L, and Haworth JC. Blood lactate and pyruvate concentrations, and their ratio during exercise in healthy children: developmental perspective. Eur J Appl Physiol 71: 518-522, 1995.
33. Putman CT, Jones NL, Hultman E, Hollidge-Horvat MG, Bonen A, McConachie DR, and Heigenhauser GJF. Effects of short-term submaximal training in humans on muscle metabolism in exercise. Am J Physiol Endocrinol Metab 275: E132-E139, 1998.
34. Rocchiccioli F, Leroux JP, and Cartier P. Quantitation of 2-ketoacids in biological fluids by gas chromatography chemical ionization mass spectrometry of o-trimethylsilyl-quinoxalinol derivatives. Biomed Mass Spectrom 8: 160-164, 1981.
35. Rokyta R Jr, Matejovic M, Novak I, Zeman V, Krouzecky A, Novak J, Trefil L, Linhartova K, and Sramek V. Submaximal exercise in healthy volunteers: the relationship between gastric mucosal and systemic energy status. Pflügers Arch 443: 852-857, 2002.
36. Romijn JA, Chinkes DL, Schwarz JM, and Wolfe RR. Lactate-pyruvate interconversion in blood: implications for in vivo tracer studies. Am J Physiol Endocrinol Metab 266: E334-E340, 1994.
37. Skelton MS, Kremer DE, Smith EW, and Gladden LB. Lactate influx into red blood cells from trained and untrained human subjects. Med Sci Sports Exerc 30: 536-542, 1998.
38. Skelton MS, Kremer DE, Smith EW, and Gladden LB. Lactate influx into red blood cells of athletic and nonathletic species. Am J Physiol Regul Integr Comp Physiol 268: R1121-R1128, 1995.
39. Stanley WC, Gertz EW, Wisneski JA, Neese RA, Morris DL, and Brooks GA. Lactate extraction during net lactate release in legs of humans during exercise. J Appl Physiol 60: 1116-1120, 1986.
40. Stanley WC, Wisneski JA, Gertz EW, Neese RA, and Brooks GA. Glucose and lactate interrelations during moderate-intensity exercise in humans. Metabolism 37: 850-858, 1988.
41. 13C-NMR studies. Biochem J 312: 75-81, 1995.
42. Tfelt-Hansen P and Siggaard-Andersen O. Lactate and pyruvate determination in 50 μl whole blood. Scand J Clin Lab Invest 27: 15-19, 1971.
43. Van Hall G, Calbet JA, Sondergaard H, and Saltin B. Similar carbohydrate but enhanced lactate utilization during exercise after 9 wk of acclimatization to 5,620 m. Am J Physiol Endocrinol Metab 283: E1203-E1213, 2002.
44. 14, purity and stability. Anal Biochem 8: 171-178, 1964.
45. Wasserman DH, Lacy DB, Bracy D, and Williams PE. Metabolic regulation in peripheral tissues and transition to increased gluconeogenic mode during prolonged exercise. Am J Physiol Endocrinol Metab 263: E345-E354, 1992.
46. Wasserman K, Beaver WL, Davis JA, Pu JZ, Heber D, and Whipp BJ. Lactate, pyruvate, and lactate-to-pyruvate ratio during exercise and recovery. J Appl Physiol 59: 935-940, 1985.
47. Wolfe RR, Jahoor F, and Miyoshi H. Evaluation of the isotopic equilibration between lactate and pyruvate. Am J Physiol Endocrinol Metab 254: E532-E535, 1988.
48. 2 and exercise on carbohydrate metabolism in the dog. Am J Physiol Endocrinol Metab 269: E409-E417, 1995.