Visualization of mitochondria in cardiomyocytes by simultaneous harmonic generation and fluorescence microscopy

Optics Express

Volumn 13, Issue 20, 2005, Pages 8263-8276

  Barzda, Virginijus ^a   Greenhalgh, Catherine ^a   Aus Der Au, Jürg ^b   Elmore, Steven ^c   Van Beek, Johannes Hgm ^c   Squier, Jeff ^d  

^a UNIVERSITY OF TORONTO   (Canada)

^b Spectra Physics Lasers Division   (United States)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

^d Department of Metallurgical and Materials Engineering   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARDIOLOGY; IMAGE ANALYSIS; MICROSCOPES; SECOND HARMONIC GENERATION;

MITOCHONDRIA; MULTIPHOTON EXCITATION FLUORESCENCE (MPF); THIRD HARMONIC (THG);

FLUORESCENCE;

EID: 30144440306     PISSN: 10944087     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OPEX.13.008263     Document Type: Article

References (37)

1. K.koing, "Multiphoton microscopy in life sciences," J Microsc-Oxford 200, 83-104 (2000).
2. L. Moreaux, O. Sandre, and J. Mertz, "Membrane imaging by second-harmonic generation microscopy," J Opt Soc Am B 17(10), 1685-1694 (2000).
3. L. Moreaux, O. Sandre, S. Charpak, M. Blanchard-Desce, and J. Mertz, "Coherent scattering in multi-harmonic light microscopy," Biophys J 80(3), 1568-1574 (2001).
4. P. J. Campagnola, M. D. Wei, A. Lewis, and L. M. Loew, "High-resolution nonlinear optical imaging of live cells by second harmonic generation," Biophys J 77(6), 3341-3349 (1999).
5. P. J. Campagnola, A. C. Millard, M. Terasaki, P. E. Hoppe, C. J. Malone, and W. A. Mohler, "Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues," Biophys. J. 82(1), 493-508 (2002).
6. R. Barille, L. Canioni, S. Rivet, L. Sarger, P. Vacher, and T. Ducret, "Visualization of intracellular Ca2+ dynamics with simultaneous two-photon-excited fluorescence and third-harmonic generation microscopes," Appl Phys Lett 79(24), 4045-4047 (2001).
7. S. W. Chu, S. Y. Chen, T. H. Tsai, T. M. Liu, C. Y. Lin, H. J. Tsai, and C. K. Sun, "In vivo developmental biology study using noninvasive multi-harmonic generation microscopy," Opt Express 11(23), 3093-3099 (2003). http://www.opticsexpress.org/abstract.cfm?URI=OPEX-11-23-3093
8. S. W. Chu, S. Y. Chen, G. W. Chern, T. H. Tsai, Y. C. Chen, B. L. Lin, and C. K. Sun, "Studies of x((2))/x((3)) tensors in submicron-scaledbio- tissues by polarization harmonics optical microscopy," Biophys. J. 86(6), 3914-3922 (2004).
9. S. W. Chu, I. H. Chen, T. M. Liu, P. C. Chen, C. K. Sun, and B. L. Lin, "Multimodal nonlinear spectral microscopy based on a femtosecond Cr : forsterite laser," Opt Lett 26(23), 1909-1911 (2001).
10. V. Barzda, C. Greenhalgh, J. Aus der Au, J. A. Squier, S. Elmore, and J. H. van Beek, "Second- and third-harmonic generation andmultiphoton excitation fluorescence microscopy for simultaneous imaging of cardiomyocytes," in Commercial ana Biomedical Applications of Ultrafast Lasers IV, J. Neev, C. B. Schaffer, and A. Ostendorf eds, Proc. of SPIE 5340, 96-103 (2004).
11. R. W. Boyd, Nonlinear optics, 2nd ed. (Academic Press, San Diego, CA, 2003).
12. I. Freund and M. Deutsch, "2nd-Harmonic Microscopy of Biological Tissue," Opt. Lett. 11(2), 94-96 (1986).
13. L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz, "Membrane imaging by simultaneous secondharmonic generation and two-photon microscopy," Opt Lett 25(5), 320-322 (2000).
14. I. Freund, M. Deutsch, and A. Sprecher, "Connective-Tissue Polarity - Optical 2nd-Harmonic Microscopy, Crossed-Beam Summation, and Small-Angle Scattering in Rat-Tail Tendon," Biophys J 50(4), 693-712 (1986).
15. Y. C. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano, "Secondharmonic tomography of tissues," Opt. Lett. 22(17), 1323-1325 (1997).
16. J. A. Squier, M. Muller, G. J. Brakenhoff, and K. R. Wilson, "Third harmonic generation microscopy," Opt Express 3(9), 315-324 (1998). http://www.opticsexpress.org/abstract.cfm?URI=OPEX-3-9-315
17. T. Y. F. Tsang, "Optical 3rd-Harmonic Generation at Interfaces," Phys Rev A 52(5), 4116-4125 (1995).
18. M. Muller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, "3D microscopy of transparent objects using thirdharmonic generation," J Microsc-Oxford 191, 266-274 (1998).
19. A. C. Millard, P. W. Wiseman, D. N. Fittinghoff, K. R. Wilson, J. A. Squier, and M. Muller, "Third-harmonic generation microscopy by use of a compact, femtosecond fiber laser source," Appl Optics 38(36), 7393-7397 (1999).
20. D. Yelin, D. Oron, E. Korkotian, M. Segal, and Y. Silbergerg, "Third-harmonic microscopy with a titaniumsapphire laser," Appl Phys B-Lasers O 74, S97-S101 (2002).
21. D. Oron, E. Tal, and Y. Silberberg, "Depth-resolved multiphoton polarization microscopy by third-harmonic generation," Opt Lett 28(23), 2315-2317 (2003).
22. S. H. Huang, A. A. Heikal, and W. W. Webb, "Two-photon fluorescence spectroscopy and microscopy of NAD(P)H and flavoprotein," Biophys J 82(5), 2811-2825 (2002).
23. C. Xu, W. Zipfel, J. B. Shear, R. M. Williams, and W. W. Webb, "Multiphoton fluorescence excitation: New spectral windows for biological nonlinear microscopy," P Natl Acad Sci USA 93(20), 10763-10768 (1996).
24. S. W. Chu, I. H. Chen, T. M. Liu, C. K. Sun, S. P. Lee, B. L. Lin, P. C. Cheng, M. X. Kuo, D. J. Lin, and H. L. Liu, "Nonlinear bio-photonic crystal effects revealed with multimodal nonlinear microscopy," J Microsc-Oxford 208, 190-200 (2002).
25. D. Kopf, F. X. Kartner, U. Keller, and K. J. Weingarten, "Diode-Pumped Mode-Locked Nd-Glass Lasers with an Antiresonant Fabry-Perot Saturable Absorber," Opt Lett 20(10), 1169-1171 (1995).
26. U. Keller, K. J. Weingarten, F. X. Kartner, D. Kopf, B. Braun, I. D. Jung, R. Fluck, C. Honninger, N. Matuschek, and J. A. derAu, "Semiconductor saturable absorber mirrors (SESAM's) for femtosecond to nanosecond pulse generation in solid-state lasers," IEEE J. Sel. Top. Quantum Electron. 2(3), 435-453 (1996).
27. C. Greenhalgh, R. Cisek, N. Prent, A. Major, J. Aus der Au, J. Squier, and V. Barzda, "Time and structural crosscorrelation image analysis of microscopic volumes, simultaneously recorded with second harmonic generation, third harmonic generation, and multiphoton excitation fluorescence microscopy," in Photonic Applications in Biosensing and Imaging, W.C.W. Chan, K. Yu, U.J. Krull, R.I. Hornsey, B.C. Wilson, and R.A. Weersink. eds., Proc. of SPIE 5969, 557-564 (2005).
28. H. L. Li, J. Suzuki, E. Bayna, F. M. Zhang, E. D. Molle, A. Clark, R. L. Engler, and W. Y. W. Lew, "Lipopolysaccharide induces apoptosis in adult rat ventricular myocytes via cardiac AT(1) receptors," Am J Physiol-Heart C 283(2), H461-H467 (2002).
29. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, "Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation," Proc. Natl. Acad. sci. USA 100(12), 7075-7080 (2003).
30. K. Blinova, C. Combs, P. Kellman, and R. S. Balaban, "Fluctuation analysis of mitochondrial NADH fluorescence signals in confocal and two-photon microscopy images of living cardiac myocytes," J. Microsc.Oxford 213, 70-75 (2004).
31. J. Eng, R. M. Lynch, and R. S. Balaban, Nicotinamide Adenine-Dinucleotide Fluorescence Spectroscopy and Imaging of Isolated Cardiac Myocytes," Biophys. J. 55(4), 621-630 (1989).
32. D. L. Farkas, M. D. Wei, P. Febbroriello, J. H. Carson, and L. M. Loew, "Simultaneous Imaging of Cell and Mitochondrial-Membrane Potentials," Biophys J 56(6), 1053-1069 (1989).
33. D. N. Romashko, E. Marban, and B. O'Rourke, "Subcellular metabolic transients and mitochondria! redox waves in heart cells," P Natl Acad sci USA 95(4), 1618-1623 (1998).
34. S. Inouâe and R. J. Walter, Video microscopy (PlenumPress, New York, 1986).
35. F. Martini and W. C. Ober, Fundamentals of anatomy & physiology, 5th ed. (Prentice Hall, Upper Saddle River, N.J. ; Toronto, 2001), p. 1 v. (various pagings).
36. L. Skarka and B. Ostadal, "Mitochondrial membrane potential in cardiac myocytes," Physiol Res 51(5), 425-434 (2002).
37. J. W. Palmer, B. Tandler, and C. L. Hoppel, "Biochemical Properties of Subsarcolemmal and Inter-Fibrillar Mitochondria Isolated from Rat Cardiac-Muscle," J. Biological Chem. 252(23), 8731-8739 (1977).