Membrane imaging by second-harmonic generation microscopy

Journal of the Optical Society of America B: Optical Physics

Volumn 17, Issue 10, 2000, Pages 1685-1694

  Moreaux, L ^a   Sandre, O ^b   Mertz, J ^a  

^a INSERM   (France)

^b INSTITUT CURIE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; DYES; FOCUSING; LASER MODE LOCKING; LIGHT POLARIZATION; MOLECULAR ORIENTATION; OPTICAL MICROSCOPY; SECOND HARMONIC GENERATION;

NUMERICAL APERTURES (NA);

IMAGING SYSTEMS;

EID: 0034365483     PISSN: 07403224     EISSN: None     Source Type: Journal    
DOI: 10.1364/JOSAB.17.001685     Document Type: Article

References (31)

1. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
2. J. R. Lakowicz and I. Gryczynski, "Multiphoton excitation of biochemical fluorophores," in Topics in Fluorescence Spectroscopy, J. R. Lakowicz, ed. (Plenum, New York, 1997), Vol. 5, p. 87.
3. C. Xu and W. W. Webb, "Multiphoton excitation of molecular fluorophores and nonlinear laser microscopy," in Topics in Fluorescence Spectroscopy, J. R. Lakowicz, ed. (Plenum, New York, 1997), Vol. 5, p. 471.
4. S. Maiti, J. B. Shear, R. M. Williams, W. R. Zipfel, and W. W. Webb, "Measuring serotonin distribution in live cells with three-photon excitation," Science 24, 530-532 (1997).
5. C. Xu, W. Zipfel, J. B. Shear, R. M. Williams, and W. W. Webb, "Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy," Proc. Natl. Acad. Sci. USA 93, 10,763-10,768 (1996).
6. J. I. Dadap, J. Shan, A. S. Weling, J. A. Misewich, A. Nahata, and T. F. Heinz, "Measurement of the vector character of electric fields by optical second-harmonic generation," Opt. Lett. 24, 1059-1061 (1999).
7. R. Gauderon, P. B. Lukins, and C. J. R. Sheppard, "Three-dimensional second-harmonic generation imaging with femtosecond laser pulses," Opt. Lett. 23, 1209-1211 (1998).
8. Y. Guo, P. P. Ho, H. Savage, D. Harris, P. Sacks, S. Schantz, F. Liu, N. Zhadin, and R. R. Alfano, "Second-harmonic tomography of tissues," Opt. Lett. 22, 1323-1325 (1997).
9. G. Peleg, A. Lewis, M. Linial, and L. M. Loew, "Non-linear optical measurement of membrane potential around single molecules at selected cellular sites," Proc. Natl. Acad. Sci. USA 96, 6700-6704 (1999).
10. P. J. Campagnola, M. Wei, A. Lewis, and L. M. Loew, "High-resolution nonlinear optical imaging of live cells by second harmonic generation," Biophys. J. 77, 3341-3349 (1999).
11. D. Yelin and Y. Silberberg, "Laser scanning third-harmonic-generation microscopy in biology," Opt. Express 5, 169-175 (1999).
12. M. Muller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, "3D-microscopy of transparent objects using third-harmonic generation," J. Microsc. 101, 266-269 (1998).
13. T. F. Heinz, H. W. K. Tom, and Y. R. Shen, "Determination of molecular orientation of monolayer adsorbates by optical second-harmonic generation," Phys. Rev. A 28, 1883-1885 (1983).
14. N. Bloembergen, "Second harmonic reflected light," Opt. Acta 13, 311-322 (1966).
15. Y. R. Shen, The Principles of Nonlinear Optics (Wiley, New York, 1984).
16. R. Boyd, Nonlinear Optics (Academic, London, 1992).
17. A. Lewis, A. Khatchatouriants, M. Treinin, Z. Chen, G. Peleg, N. Friedman, O. Bouetvich, Z. Rothman, L. Loew, and M. Sheres, "Second-harmonic generation of biological interfaces: probing the membrane protein bacteriorhodopsin and imaging membrane potential around GFP molecules at specific sites in neuronal cells of C. elegans," Chem. Phys. 245, 133-144 (1999).
18. L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz, "Membrane imaging by simultaneous second-harmonic generation and two photon microscopy," Opt. Lett. 25, 320-322 (2000).
19. A. Willets, J. E. Rice, D. Burland, and D. P. Shelton, "Problems in the comparison of theoretical and experimental hyperpolarizabilities," J. Chem. Phys. 97, 7590-7599 (1992).
20. C. Xu and W. W. Webb, "Measurement of two-photon excitation cross section of molecular fluorophores with data from 690 nm to 1050 nm," J. Opt. Soc. Am. B 18, 481-491 (1996).
21. N. Bloembergen, Nonlinear Optics, 4th ed. (World Scientific, Singapore, 1965).
22. M. Born and E. Wolf, Principles of Optics, 6th ed. (Pergamon, Oxford, 1993).
23. J. Mertz, "Molecular photodynamics involved in multiphoton excitation fluorescence microscopy," Eur. Phys. J. D 3, 53-66 (1998).
24. S. R. Marder, D. N. Beratan, and L.-T. Cheng, "Approaches for optimizing the first hyperpolarizability of conjugated organic molecules," Science 252, 103-106 (1991).
25. T. Kogej, D. Beljonne, F. Meyers, J. W. Perry, S. R. Marder, and J. L. Brédas, "Mechanisms for enhancement of two-photon absorption in donor-acceptor conjugated chromophores," Chem. Phys. Lett. 298, 1-6 (1998).
26. D. S. Chemla and J. Zyss, Nonlinear Optical Properties of Organic Molecules and Crystals (Academic, New York, 1984).
27. J. L. Oudar, "Optical nonlinearities of conjugated molecules. Stilbene derivatives and highly polar aromatic compounds," J. Chem. Phys. 67, 446-457 (1977).
28. L. M. Loew and L. L. Simpson, "Charge shift probes of membrane potential. A probable electrochromic mechanism for ASP probes on a hemispherical lipid bilayer," Bio-phys. J. 34, 353-365 (1981).
29. O. Sandre, L. Moreaux, and F. Brochard, "Dynamics of transient pores in stretched vesicles," Proc. Natl. Acad. Sci. USA 96, 10,588-10,596 (1999).
30. C. W. Dirk, R. J. Twieg, and G. Wagniére, "The contribution of π electrons to second harmonic generation in organic molecules," J. Am. Chem. Soc. 108, 5387-5395 (1986).
31. B. Richards and E. Wolf, "Electromagnetic diffraction in optical systems. II. Structure of the image field in aplanetic system," Proc. R. Soc. London, Ser. A 253, 358-379 (1959).