3D microscopy of transparent objects using third-harmonic generation

Journal of Microscopy

Volumn 191, Issue 3, 1998, Pages 266-274

  Muller M ^a   Squier, J ^b   Wilson, K R ^c   Brakenhoff, G J ^a  

^a Institute for Molecular Cell Biology   (Netherlands)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Confocal microscopy; Multiphoton excitation; Third harmonic generation

Indexed keywords

HARMONIC ANALYSIS; NONLINEAR OPTICS; REFRACTIVE INDEX;

3-D MICROSCOPY; EXCITATION POWER; MULTIPHOTON EXCITATION; NONLINEAR DEPENDENCE; NONRESONANT; OPTICAL SECTIONING; THIRD-HARMONIC GENERATION; THIRD-ORDER NONLINEAR SUSCEPTIBILITY; THREE DIMENSIONAL IMAGING; TRANSPARENT OBJECTS;

HARMONIC GENERATION;

ARTICLE; CONFOCAL MICROSCOPY; IMAGE ANALYSIS; IMAGE RECONSTRUCTION; MICROSCOPY; PRIORITY JOURNAL; REFRACTION INDEX; THREE DIMENSIONAL IMAGING;

EID: 0031667172     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1998.00399.x     Document Type: Article

References (19)

1. Barad, Y., Eisenberg, H., Horowitz, M. & Silberberg, Y. (1997) Nonlinear scanning laser microscopy by third-harmonic generation. Appl. Phys. Lett. 70, 922-924.
2. Boyd, R.W. (1992) Nonlinear Optics. Academic Press, Boston.
3. Campagnola, P.J., Young, D.W., Cowan, A.K., Roychoudhuri, C. & Loew, L.M. (1997) Applications in nonlinear optical microscopy. Cell Vision, 4, 191-192.
4. Denk, W., Strickler, J.H. & Webb, W.W. (1990) Two-photon laser scanning fluorescence microscopy. Science, 248, 75-76.
5. Eramo, R. & Matera, M. (1994) Third-harmonic generation in positively dispersive gases with a novel cell. Appl. Opt. 33, 1691-1696.
6. Gryczynski, I., Malak, H. & Lakowicz, J.R. (1996) Multiphoton excitation of the DNA stains DAPl1 and Hoechst. Bioimaging, 4, 138-148.
7. Hell, S.W., Bahlmann, K., Schrader, M., Soini, A., Malak, H., Gryczynski, I. & Lakowicz, J.R. (1996) Three-photon excitation in fluorescence microscopy. J. Biomed. Opt. 1, 71-74.
8. Hell, S.W. & Wichmann, J. (1994) Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt. Lett. 19, 780-782.
9. Hellwarth, R. & Christensen, P. (1974) Nonlinear optical microscopic examination of structure in polycrystalline ZnSc. Opt. Commun. 12, 318-522.
10. Largo, A., Hilber, G. & Wallenstein, R. (1987) Optical frequency conversion in gaseous media. Phys. Rev. A, 36, 5827-5836.
11. Müller, M., Squier, J. & Brakenhoff, G.J. (1995) Measurement of femtosecond pulses in the focal point of a high-numericalaperture lens by two-photon absorption. Opt. Lett. 20, 1038-1040.
12. Müller, M., Squier, J., Wolleschensky, R., Simon, U. & Brakenhoff, G.J. (1998) Dispersion pre-compensation of 15 femtosecond optical pulses for high-numerical-aperture objectives. J. Microsc: 191, 141-158.
13. Piston, D.W. & Webb, W.W. (1991) Three dimensional imaging of intracellular calcium activity using two-photon excitation of fluorescent indicator dye Indo-1. Biophys. J. 59, 156.
14. Sucha, G. (1998) Doped optical fibers promise compact femtosecond sources. Laser Focus World. April, 121.
15. Tsang, T.Y.F. (1995) Optical third-harmonic generation at interfaces. Phys. Rev. A, 52, 4116-4125.
16. van der Voort, H.T.M., Messerli, J.M., Noordmans, H.J. & Smeulders, A.W.M. (1993) Volume visualisation for interactive microscopic analysis. Bioimaging, 1, 20-29.
17. Ward, J.F. & New, G.H.C. (1969) Optical third harmonic generation in gases by a focused laser beam. Phys. Rev. 185, 57-72.
18. Webb, W.W. (1990) Two photon excitation in laser scanning fluorescence microscopy. Transactions of the Royal Microscopical Society (ed. by H. Y. Elder), pp. 445-450. Institute of Physics Publishing, Bristol.
19. Wilson, K.R. & Yakovlev, V.V. (1996) Ultrafast rainbow: tunable ultrashort pulses from a solid-state kilohertz system. J. Opt. Soc. Am. B, 14, 444-448.