Anucleate cell blue assay: A useful tool for identifying novel type II topoisomerase inhibitors

Antimicrobial Agents and Chemotherapy

Volumn 50, Issue 1, 2006, Pages 348-350

  Oyamada, Yoshihiro ^a   Ito, Hideaki ^a   Fujimoto Nakamura, Mika ^a   Tanitame, Akihiko ^a   Iwai, Noritaka ^b   Nagai, Kazuo ^c   Yamagishi, Jun Ichi ^a   Wachi, Masaaki ^b  

^a DAINIPPON SUMITOMO PHARMA CO LTD   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c CHUBU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING); DNA TOPOISOMERASE IV; GYRASE INHIBITOR; NOVOBIOCIN; QUINOLINE DERIVED ANTIINFECTIVE AGENT; SPARFLOXACIN;

ANTIBACTERIAL ACTIVITY; ANUCLEATE CELL BLUE ASSAY; ARTICLE; BACTERIAL STRAIN; CELL ASSAY; DRUG STRUCTURE; ENZYME INHIBITION; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS;

BACTERIA; BIOLOGICAL ASSAY; COLORING AGENTS; DNA TOPOISOMERASES, TYPE II; ENZYME INHIBITORS; FLUOROQUINOLONES;

EID: 29944447456     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.50.1.348-350.2006     Document Type: Article

References (13)

1. Fairweather, N. F., E. Orr, and I. B. Holland. 1980. Inhibition of deoxyribonucleic acid gyrase: effects on nucleic acid synthesis and cell division in Escherichia coli K-12. J. Bacteriol. 142:153-161.
2. Fujimoto-Nakamura, M., H. Ito, Y. Oyamada, T. Nishino, and J.-I. Yamagishi. 2005. Accumulation of mutations in both gyrB and parE genes is associated with high-level resistance to novobiocin in Staphylococcus aureus. Antimicrob. Agents Chemother. 49:3810-3815.
3. Gitai, Z., N. A. Dye, A. Reisenauer, M. Wachi, and L. Shapiro. 2005. MreB actin-mediated segregation of a specific region of a bacterial chromosome. Cell 120:329-341.
4. Hiasa, H., D. O. Yousef, and K. J. Marians. 1996. DNA strand cleavage is required for replication fork arrest by a frozen topoisomerase-quinolone-DNA ternary complex. J. Biol. Chem. 271:26424-26429.
5. Hiraga, S., H. Niki, T. Ogura, C. Ichinose, H. Mori, B. Ezaki, and A. Jaffe. 1989. Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells. J. Bacteriol. 171:1496-1505.
6. Iwai, N., K. Nagai, and M. Wachi. 2002. Novel S-benzylisothiourea compound that induces spherical cells in Escherichia coli probably by acting on a rod-shape-determining protein(s) other than penicillin-binding protein 2. Biosci. Biotechnol. Biochem. 66:2658-2662.
7. Khodursky, A. B., B. J. Peter, M. B. Schmid, J. DeRisi, D. Botstein, P. O. Brown, and N. R. Cozzarelli. 2000. Analysis of topoisomerase function in bacterial replication fork movement: use of DNA microarrays. Proc. Natl. Acad. Sci. USA 97:9419-9424.
8. Levine, C., H. Hiasa, and K. J. Marians. 1998. DNA gyrase and topoisomerase IV: biochemical activities, physiological roles during chromosome replication, and drug sensitivities. Biochim. Biophys. Acta 1400:29-43.
9. Lewis, R. J., O. M. Singh, C. V. Smith, T. Skarzynski, A. Maxwell, A. J. Wonacott, and D. B. Wigley. 1996. The nature of inhibition of DNA gyrase by the coumarins and the cyclothialidines revealed by X-ray crystallography. EMBO J. 15:1412-1420.
10. Peng, H., and K. J. Marians. 1993. Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions. J. Biol. Chem. 268:24481-24490.
11. Sato, K., Y. Inoue, T. Fujii, H. Aoyama, M. Inoue, and S. Mitsuhashi. 1986. Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli. Antimicrob. Agents Chemother. 30:777-780.
12. 2+ chelator, EGTA. Biochimie 81:909-913.
13. Yamagishi, J.-I., T. Kojima, Y. Oyamada, K. Fujimoto, H. Hattori, S. Nakamura, and M. Inoue. 1996. Alterations in the DNA topoisomerase IV grlA gene responsible for quinolone resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 40:1157-1163.