Oligomeric structure of mammalian purine nucleoside phosphorylase in solution determined by analytical ultracentrifugation

Zeitschrift fur Naturforschung - Section C Journal of Biosciences

Volumn 60, Issue 11-12, 2005, Pages 927-931

  Behlke, Joachim ^a   Koellner, Gertraud ^b   Bzowska, Agnieszka ^c  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

^c UNIVERSITY OF WARSAW   (Poland)

Author keywords

Analytical Ultracentrifugation; Oligomeric State; Purine Nucleoside Phosphorylase (PNP)

Indexed keywords

MAMMALIA;

EID: 29744455848     PISSN: 09395075     EISSN: None     Source Type: Journal    
DOI: 10.1515/znc-2005-11-1217     Document Type: Article

References (18)

1. Ames B. N. (1966), Assay for inorganic phosphate, total phosphate and phosphonates. Methods Enzymol. 8, 115-116.
2. Bantia S., Miller P. J., Parker C. D., Ananth S. L., Horn L. L., Babu Y. S., and Sandhu J. S. (2002), Comparison of in vivo efficacy of BCX-1777 and cyclosporin in xenogeneic graft-vs.-host disease: the role of dGTP in antiproliferative action of BCX-1777. Int. Immunopharmacol. 2, 913-923.
3. Bantia S., Ananth S. L., Parker C. D., Horn L. L., and R. Upshaw R. (2003), Mechanism of inhibition of T-acute lymphoblastic leukemia cells by PNP inhibitor-BCX-1777. Int. Immunopharmacol. 3, 879-887.
4. Behlke J. and Ristau O. (1997), Analysis of interacting biopolymer systems by analytical ultracentrifugation. Biophys. J. 72, 428-432.
5. Behlke J., Ristau O., and Schönfeld H.-J. (1997), Nucleotide-dependent complex formation between the Escherichia coli chaperonins GroEL and GroES studied under equilibrium conditions. Biochemistry 36, 5149-5156.
6. rd J. A., Sorscher E. J., Townes T. M., Waud W. R., and Ealick S. E. (2003), Designer gene therapy using an Escherichia coli purine nucleoside phosphorylase/prodrug system. Chem. Biol. 10, 1173-1181.
7. Bzowska A. (2002), Calf spleen purine nucleoside phosphorylase: complex kinetic mechanism, hydrolysis of 7-methylguanosine, and oligomeric state in solution. Biochim. Biophys. Acta 1596, 293-317.
8. Bzowska A., Kulikowska E., and Shugar D. (2000), Purine nucleoside phosphorylases: properties, functions and clinical aspects. Pharmacol. Ther. 88, 349-425.
9. Bzowska A., Koellner G., Wielgus-Kutrowska B., Stroh A., Raszewski G., Holý A., Steiner T., and Frank J. (2004), Crystal structure of calf spleen purine nucleoside phosphorylase with two full trimers in the asymmetric unit: important implications for the mechanism of catalysis. J. Mol. Biol. 342, 1015-1032.
10. Cohen R., Giraud B., and Messiah A. (1967), Theory and practice of the analytical centrifugation of an active substrate-enzyme complex. Biopolymers 5, 203-225.
11. Fedorov A., Shi W., Kicska G., Fedorov E., Tyler P. C., Furneaux R. H., Hanson J. C., Gainsford G. J., Larese L. Z., Schramm V. L., and Almo S. C. (2001), Transition state structure of purine nucleoside phosphorylase and principles of atomic motion in enzymatic catalysis. Biochemistry 40, 853-860.
12. Freitas da Silveira N. J., Uchoa H. B., Canduri F., Pereira J. H., Camera Jr. J. C., Basso L. A., Palma M. S., Santos D. S., and de Azevedo Jr. W. F. (2004), Structural bioinformatics study of PNP from Schistosoma mansoni. Biochem. Biophys. Res. Commun. 322, 100-104.
13. Kemper D. and Everse J. (1973), Active enzyme sedimentation. Methods. Enzymol. 27, 67-83.
14. Kulikowska E., Bzowska A., Wierzchowski J., and Shugar D. (1986), Properties of two unusual, and fluorescent substrates of purine-nucleoside phosphorylase: 7-methylguanosine and 7-methylinosine. Biochim. Biophys. Acta 874, 355-366.
15. Luić M., Koellner G., Shugar D., Saenger W., and Bzowska A. (2001), Calf spleen purine nucleoside phosphorylase: structure of its ternary complex with an N(7)-acycloguanosine inhibitor and a phosphate anion. Acta Cryst. D57, 30-36.
16. Mao C., Cook W. J., Zhou M., Federov A. A., Almo S. C., and Ealick S. E. (1998), Calf spleen purine nucleoside phosphorylase complexed with substrates and substrate analogues. Biochemistry 37, 7135-7146.
17. Ropp P. A. and Traut T. W. (1991), Purine nucleoside phosphorylase. Allosteric regulation of a dissociating enzyme. J. Biol. Chem. 266, 7682-7687.
18. Shi W., Ting L. M., Kicska G. A., Lewandowicz A., Tyler P. C., Evans G. B., Furneaux R. H., Kim K., Almo S. C., and Schramm V. L. (2004), Plasmodium falciparum purine nucleoside phosphorylase: crystal structures, immucillin inhibitors, and dual catalytic function. J. Biol. Chem. 279, 18103-18106.