In vitro elucidation of substrate specificity and bioassay of proprotein convertase 4 using intramolecularly quenched fluorogenic peptides

Biochemical Journal

Volumn 380, Issue 2, 2004, Pages 505-514

  Basak, Sarmistha ^a   Chrétien, Michel ^a   Mbikay, Majambu ^a   Basak, Ajoy ^a  

^a OTTAWA HOSPITAL RESEARCH INSTITUTE   (Canada)

Author keywords

ADAM proteins; Cleavage specificity; Enzyme bioassay; Intramolecularly quenched fluorogenic substrates; Kinetic parameters; Proprotein convertase 4; Substrate specificity

Indexed keywords

AMINO ACIDS; BIOASSAY; BIOCHEMISTRY; FLUORESCENCE; MOLECULAR BIOLOGY; MUTAGENESIS; POLYPEPTIDES; QUENCHING; SUBSTRATES;

CYSTEINES; GERM CELLS; KINETIC ANALYSIS; MACROPHAGES;

ENZYMES;

ADAM PROTEIN; HYPOPHYSIS ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE; MUTANT PROTEIN; PEPTIDE DERIVATIVE; PROPROTEIN CONVERTASE 4; PROPROTEIN CONVERTASE 7; PROTEIN; SERINE PROTEINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BIOASSAY; CONTROLLED STUDY; ENZYME KINETICS; ENZYME SPECIFICITY; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN VARIANT; SPERM;

ANIMALS; BIOLOGICAL ASSAY; COUMARINS; FLUORESCENT DYES; HUMANS; MALE; MICE; MICE, KNOCKOUT; PEPTIDES; PROPROTEIN CONVERTASES; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; SOMATOMEDINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBSTRATE SPECIFICITY; SUBTILISINS;

ANIMALIA; MAMMALIA;

EID: 2942716755     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031405     Document Type: Article

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