A single amino acid substitution can shift the optimum pH of DNase I for enzyme activity: Biochemical and molecular analysis of the piscine DNase I family

Biochimica et Biophysica Acta - General Subjects

Volumn 1672, Issue 3, 2004, Pages 174-183

  Yasuda, T ^a   Takeshita, H ^b   Iida, R ^a   Ueki, M ^a   Nakajima, T ^b   Kaneko, Y ^b   Mogi, K ^b   Kominato, Y ^b   Kishi, K ^b  

^a UNIVERSITY OF FUKUI   (Japan)

^b GUNMA UNIVERSITY   (Japan)

Author keywords

cDNA cloning; Deoxyribonuclease I; Fish; Molecular evolution; Optimum pH; Purification

Indexed keywords

AMINO ACID; ASPARTIC ACID; COMPLEMENTARY DNA; DEOXYRIBONUCLEASE I; ENZYME; HISTIDINE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMPHIBIA; ANGUILLA JAPONICA; ANIMAL TISSUE; ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; CLASSIFICATION; COMPARATIVE STUDY; CONTROLLED STUDY; CRYPRUS CARPIO; EEL; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STRUCTURE; FISH; GENETIC CODE; MAMMAL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OREOCHROMIS MOSSAMBICA; PAGRUS MAJOR; PH; PRIORITY JOURNAL; PROTEIN FAMILY; REPTILE; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; TAKIFUGU RUBRIPES; VERTEBRATE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CELL LINE; DEOXYRIBONUCLEASE I; DNA, COMPLEMENTARY; ENZYME STABILITY; FISHES; HEAT; HEPATOPANCREAS; HUMANS; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PANCREAS; PHYLOGENY; SEQUENCE ALIGNMENT;

AMPHIBIA; ANGUILLA JAPONICA; JAPONICA; MAMMALIA; OREOCHROMIS; PAGRUS; PAGRUS MAJOR; PISCES; REPTILIA; TAKIFUGU; TAKIFUGU RUBRIPES; VERTEBRATA;

EID: 2942538267     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbagen.2004.03.012     Document Type: Article

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