Mutational analysis of human DNase I at the DNA binding interface: Implications for DNA recognition, catalysis, and metal ion dependence

Protein Science

Volumn 7, Issue 3, 1998, Pages 628-636

  Pan, Clark Q ^a   Ulmer, Jana S ^a   Herzka, Andrea ^a   Lazarus, Robert A ^a  

^a GENENTECH INC   (United States)

Author keywords

DNase I; Protein DNA interactions; Site directed mutagenesis; Structure function analysis

Indexed keywords

ARGININE; BACTERIAL ENZYME; DEOXYRIBONUCLEASE I; DNA; GLUTAMIC ACID; GLUTAMINE; METAL ION; SPHINGOMYELIN PHOSPHODIESTERASE; TYROSINE;

ARTICLE; CATTLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYSTIC FIBROSIS; DNA DEGRADATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; HUMAN; HUMAN CELL; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

EID: 0031935579     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070312     Document Type: Article

References (36)

1. Cacia J, Quan CP, Vasser M, Sliwkowski MB, Frenz J. 1993. Protein sorting by high-performance liquid chromatography. J Chromatog 634:229-239.
2. Campbell VW, Jackson DA. 1980. The effect of divalent cations on the mode of action of DNase I. J Biol Chem 255:3726-3735.
3. Doherty AJ, Worrall AF, Connolly BA. 1991. Mutagenesis of the DNA binding residues in bovine pancreatic DNase 1: An investigation into the mechanism of sequence discrimination by a sequence selective nuclease. Nucleic Acids Res 19:6129-6132.
4. Doherty AJ, Worrall AF, Connolly BA. 1995. The roles of Arginine 41 and Tyrosine 76 in the coupling of DNA recognition to phosphodiester bond cleavage by DNase I: A study using site-directed mutagenesis. J Mol Biol 257:366-377.
5. Ferrin TE, Huang CL, Jarvis LE, Langridge R. 1988. The MIDAS display system. J Mol Graph 6:13-27.
6. Fuchs HJ, Borowitz DS, Christiansen DH, Morris EM, Nash ML, Ramsey BW, Rosenstein BJ, Smith AL, Wohl ME. 1994. Effect of aerosolized recombinant human DNase on exacerbations of respiratory symptoms and on pulmonary function in patients with cystic fibrosis. N Engl J Med 331:631-642.
7. Jones SJ, Worrall AF, Connolly BA. 1996. Site-directed mutagenesis of the catalytic residues of bovine pancreatic deoxyribonuclease I. J Mol Biol 264:1154-1163.
8. Lahm A, Suck D. 1991. DNase I-induced DNA conformation: 2 A structure of a DNase I-octamer complex. J Mol Biol 227:645-667.
9. Laskowski M Sr. 1971. In: Boyer, PD, ed. The enzymes. New York: Academic Press, pp 289-311.
10. 1 hybrid mice: Studies with recombinant murine DNase and with dexamethasone. Clin Exp Immunol 106:243-252.
11. Matsuo Y, Yamada A, Tsukamoto K, Tamura H, Ikezawa H, Nakamura H, Nishikawa K. 1996. A distant evolutionary relationship between bacterial sphingomyelinase and mammalian DNase I. Protein Sci 5:2459-2467.
12. Melgar E, Goldthwait DA. 1968. Deoxyribonucleic acid nucleases II. The effects of metals on the mechanism of action of deoxyribonuclease I. J Biol Chem 243:4409-4416.
13. Moore S. 1981. In: Boyer, PD, ed. The enzymes. New York: Academic Press. pp 281-296.
14. Oefner C, Suck D. 1986. Crystallographic refinement and structure of DNase I at 2 Å resolution. J Mol Biol 192:605-632.
15. Pan CQ, Lazarus RA. 1997. Engineering hyperactive variants of human deoxyribonuclease I by altering its functional mechanism. Biochemistry 36:6624-6632.
16. Pan CQ, Lazarus RA. 1998. Hyperactivity of human DNase I variants: Dependence on the number of positively charged residues and concentration, length, and environment of DNA. J Biol Chem, in press.
17. Parrish JE, Ciccodicola A, Wehnert M, Cox GF, Chen E, Nelson DL. 1995. A muscle specific DNase I-like gene in human Xq28. Human Mol Genet 4:1557-1564.
18. Paudel HK, Liao TH. 1986. Comparison of the three primary structures of deoxyribonuclease isolated from bovine, ovine, and porcine pancreas. J Biol Chem 267:16012-16017.
19. Peitsch MC, Irmler M, French LE, Tschopp J. 1995. Genomic organization and expression of mouse deoxyribonuclease I. Biochem Biophys Res Commun 207:62-68.
20. Pergolizzi R, Appierto V, Bosetti A, DeBellis G, Rovida E, Biunno I. 1996. Cloning of a gene encoding a DNase I-like endonuclease in the human Xq28 region. Gene 165:267-270.
21. a values of two histidine residues in human haemoglobin, the Bohr effect, and the dipole moments of alpha-helices. J Mol Biol 183:491-498.
22. Polzer B, Mannherz HG. 1990. Nucleotide sequence of a full length cDNA clone encoding the deoxyribonuclease I (DNase I) from rat parotid gland. Nucleic Acids Res 18:7151.
23. 2+ in the activity of bovine pancreatic deoxyribonuclease. J Biol Chem 250:1981-1986.
24. Ramsey BW. 1996. Management of pulmonary disease in patients with cystic fibrosis. N Engl J Med 335:179-188.
25. Ramsey BW, Astley SJ, Aitken ML, Burke W, Colin AA, Dorkin HL, Eisenberg JD, Gibson RL, Harwood IR, Schidlow DV, Wilmott RW, Wohl ME, Meyerson LJ, Shak S, Fuchs H, Smith AL. 1993. Efficacy and safety of shortterm administration of aerosolized recombinant human deoxyribonuclease ' in patients with cystic fibrosis. Am Rev Respir Dis 148:145-151.
26. Rodriguez AM, Rodin D, Nomura H, Morton CC, Stanislawa W, Schneider MC. 1997. Identification, localization, and expression of two novel human genes similar to deoxyribonuclease I. Genomics 42:507-513.
27. Shak S, Capon DJ, Hellmiss R, Marsters SA, Baker CL. 1990. Recombinant human DNase I reduces the viscosity of cystic fibrosis sputum. Proc Natl Acad Sci USA 87:9188-9192.
28. Sinicropi D, Baker DL, Prince WS, Shiffer K, Shak S. 1994. Colorimetric determination of DNase I activity with a DNA-methyl green substrate. Anal Biochem 222:351-358.
29. Suck D. 1994. DNA recognition by DNase I. J Mol Recog 7:65-70.
30. Suck D, Oefner C. 1986. Structure of DNase I at 2.0 Å resolution suggests a mechanism for binding to and cutting DNA. Nature 321:620-625.
31. Ulmer JS, Herzka A. Toy KJ, Baker DL, Dodge AH, Sinicropi D, Shak S, Lazarus RA. 1996. Engineering actin-resistant human DNase I for treatment of cystic fibrosis. Proc Natl Acad Sci USA 93:8225-8229.
32. Warren MA, Evans SJ, Connolly BA. 1997. Effects of non-conservative changes to tyrosine 76, a key DNA binding residue of DNase I, on phosphodiester bond cleavage and DNA hydrolysis selectivity. Protein Eng 10:279-283.
33. Weston SA, Lahm A, Suck D. 1992. X-ray structure of the DNase I-d(GG TATACC)2 complex at 2.3 Å resolution. J Mol Biol 226:1237-1256.
34. Wolf E, Frenz J, Suck D. 1995. Structure of human pancreatic DNase I at 2.2 Å resolution. Protein Eng 8 (Suppl.):79.
35. Worrall AF, Connolly BA. 1990. The chemical synthesis of a gene coding for bovine pancreatic DNase I and its cloning and expression in Escherichia coli. J Biol Chem 265:21889-21895.
36. Zeng Z, Parmalee D, Hyaw H, Coleman TA, Su K, Zhang J, Gentz R, Ruben S, Rosen C, Li Y. 1997. Cloning and characterization of a novel human DNase. Biochem Biophys Res Commun 231:499-504.