Purification and characterization of VanXYc, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174

European Journal of Biochemistry

Volumn 269, Issue 11, 2002, Pages 2740-2746

  Podmore, Adrian H B ^a,b   Reynolds, Peter E ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b BIO PRODUCTS LABORATORY   (United Kingdom)

Author keywords

D,D carboxypeptidase; D,D dipeptidase; Enterococcus gallinarum; Vancomycin resistance

Indexed keywords

ALANINE; ASPARTIC ACID; CARBOXYPEPTIDASE; DIPEPTIDASE; HISTIDINE; LYSINE; MALTOSE BINDING PROTEIN; PENTAPEPTIDE; PEPTIDOGLYCAN; SERINE; URIDINE DIPHOSPHATE; VANCOMYCIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENTEROCOCCUS GALLINARUM; ENZYME ANALYSIS; ENZYME PURIFICATION; HYDROLYSIS; KINETICS; MICHAELIS CONSTANT; MUTATION; NONHUMAN; PRIORITY JOURNAL;

BACTERIAL PROTEINS; CARBOXYPEPTIDASES; DIPEPTIDASES; ENTEROCOCCUS; KINETICS; MEMBRANE PROTEINS; MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; RECOMBINANT FUSION PROTEINS; SERINE-TYPE D-ALA-D-ALA CARBOXYPEPTIDASE; SUBSTRATE SPECIFICITY; VANCOMYCIN RESISTANCE;

ENTEROCOCCUS; ENTEROCOCCUS GALLINARUM; NEGIBACTERIA;

EID: 0036268443     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.02946.x     Document Type: Article

References (32)

1. Structure, biochemistry and mechanism of action of glycopeptide antibiotics
2. Glycopeptide resistance in enterococci
3. Structural relationship between the vancomycin-resistance protein VanH and 2-hydroxycarboxylic acid dehydrogenases
4. Molecular basis for vanco-mycin resistance in Enterococcus faecium BM4147: Biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA
5. Identification of vancomycin resistance protein VanA as a D-alanine: D-alanine ligase of altered substrate specificity
6. Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine
7. The VanS-VanR two-component regulatory system controls synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecalis BM4174
8. Regulation of vanB-type vancomycin resistance gene-expression by the VanS (B)-VanR (B) 2-component regulatory system in Enterococcus faecalis V583
9. Conjugal transfer of the vancomycin resistance determinant VanB between enterococci involves the movement of large genetic elements from chromosome to chromosome
10. Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine: D-alanine ligase-related protein necessary for vancomycin resistance
11. Analysis of genes encoding D-alanine: D-alanine ligase-related enzymes in Enterococcus casseliflavus and Enterococcus flavescens
12. Modification of peptidoglycan precursors is a common feature of the low-level vancomycin-resistant VanB-type Enterococcus D366 and of the naturally glycopeptide-resistant species Lactobacillus casei, Pediococcus pentosaceus, Leuconostoc mesenteroides, and Enterococcus gallinarum
13. Analysis of peptidoglycan precursors in vancomycin-resistant Enterococcus gallinarum BM4174
14. Bacterial resistance to vancomycin: Overproduction, purification and characterization of VanC-2 from Enterococcus casseliflavus as a D-Ala: D-Ser ligase
15. Gene vanXYC encodes D,D-dipeptidase (VanX) and D,D-carboxypeptidase (VanY) activities in vancomycin-resistant Enterococcus gallinarum BM4174
16. Characterization and modelling of VanT: A novel, membrane-bound, serine racemase from vancomycin-resistant Enterococcus gallinarum BM4174
17. Overexpression, purification, and characterization of VanX, a D,D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147
18. Requirement of the VanY and VanX D,D-peptidases for glycopeptide resistance in enterococci
19. The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance
20. Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX
21. Homologs of the vancomycin resistance D-Ala-D-Ala dipeptidase VanX in Streptomyces toyocaensis, Escherichia coli and Synecho-cystis: Attributes of catalytic efficiency, stereoselectivity and regulation with implications for function
22. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC18 vectors
23. DNA sequencing with chain-terminating inhibitors
24. A rapid and sensitive method for rapid quantification of microgram quantities of protein utilising a principle of protein-dye binding
25. Modified peptidoglycan precursors produced by glycopeptide-resistant enterococci
26. Expression and purification of maltose-binding protein fusions
27. Over-production, purification, and properties of the uridine-diphosphate-N-acetylmuramyl:L-alanine ligase from Escherichia coli
28. 6-tag and maltose-binding protein double-affinity fusion system
29. VanX, a metalloenzyme conferring high-level vancomycin resistance
30. A new graphical procedure for estimating enzyme kinetic parameters
31. Mutational analysis of active-site residues of the enterococcal D-Ala-D-Ala dipeptidase VanX and comparison with Escherichia coli D-Ala-D-Ala ligase and D-Ala-D-Ala carboxypeptidase VanY