Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR

International Journal of Infectious Diseases

Volumn 10, Issue 1, 2006, Pages 32-37

  Keyaerts, Els ^a   Vijgen, Leen ^a   Maes, Piet ^a   Duson, Griet ^a   Neyts, Johan ^a   Van Ranst, Marc ^a,b  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

^b UNIVERSITY HOSPITALS LEUVEN   (Belgium)

Author keywords

cRNA standard; Quantitative PCR; Real time PCR; RT PCR; SARS CoV; TaqMan PCR

Indexed keywords

NEUROENDOCRINE SPECIFIC PROTEIN; RNA POLYMERASE; VIRUS RNA;

ARTICLE; BIOTECHNOLOGY; COMPARATIVE STUDY; DIAGNOSTIC PROCEDURE; DISEASE SEVERITY; GENETIC TRANSCRIPTION; NONHUMAN; PROTEIN DOMAIN; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SARS CORONAVIRUS; SENSITIVITY ANALYSIS; SEVERE ACUTE RESPIRATORY SYNDROME; VIRUS GENOME; VIRUS LOAD;

ANIMALS; CERCOPITHECUS AETHIOPS; CORONAVIRUS; CORONAVIRUS 229E, HUMAN; CORONAVIRUS OC43, HUMAN; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RNA REPLICASE; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEVERE ACUTE RESPIRATORY SYNDROME; VERO CELLS;

EID: 29344455469     PISSN: 12019712     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijid.2005.02.003     Document Type: Article

References (20)

1. J.S. Peiris, Y. Guan, and K.Y. Yuen Severe acute respiratory syndrome Nat Med 10 2004 S88 S97
2. C. Drosten, S. Gunther, W. Preiser, S. van der Werf, H.R. Brodt, and S. Becker Identification of a novel coronavirus in patients with severe acute respiratory syndrome N Engl J Med 348 2003 1967 1976
3. T.G. Ksiazek, D. Erdman, C.S. Goldsmith, S.R. Zaki, T. Peret, S. Emery SARS Working Group A novel coronavirus associated with severe acute respiratory syndrome N Engl J Med 348 2003 1953 1966
4. T. Kuiken, R.A. Fouchier, M. Schutten, G.F. Rimmelzwaan, G. van Amerongen, and D. van Riel Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome Lancet 362 2003 263 270
5. J.S. Peiris, S.T. Lai, L.L. Poon, Y. Guan, L.Y. Yam, W. Lim SARS study group Coronavirus as a possible cause of severe acute respiratory syndrome Lancet 361 2003 1319 1325
6. P.A. Rota, M.S. Oberste, S.S. Monroe, W.A. Nix, R. Campagnoli, and J.P. Icenogle Characterization of a novel coronavirus associated with severe acute respiratory syndrome Science 300 2003 1394 1399
7. M.A. Marra, S.J. Jones, C.R. Astell, R.A. Holt, A. Brooks-Wilson, and Y.S. Butterfield The genome sequence of the SARS-associated coronavirus Science 300 2003 1399 1404
8. S.M. Poutanen, D.E. Low, B. Henry, S. Finkelstein, D. Rose, K. Green National Microbiology Laboratory, Canada, Canadian Severe Acute Respiratory Syndrome Study Team Identification of severe acute respiratory syndrome in Canada N Engl J Med 348 2003 1995 2005
9. L.L. Poon, K.H. Chan, O.K. Wong, W.C. Yam, K.Y. Yuen, and Y. Guan Early diagnosis of SARS-coronavirus infection by real-time RT-PCR J Clin Virol 28 2003 233 238
10. E.K. Ng, D.S. Hui, K.C. Chan, E.C. Hung, R.W. Chiu, and N. Lee Quantitative analysis and prognostic implication of SARS-coronavirus RNA in the plasma and serum of patients with severe acute respiratory syndrome Clin Chem 49 2003 1976 1980
11. J.S. Peiris, C.M. Chu, V.C. Cheng, K.S. Chan, I.F. Hung, L.L. Poon HKU/UCH SARS Study Group. Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study Lancet 361 2003 1767 1772
12. S. Fronhoffs, G. Totzke, S. Stier, N. Wernert, M. Rothe, and T. Bruning A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction Mol Cell Probes 16 2002 99 110
13. L.T. Lau, Y.W. Fung, F.P. Wong, S.S. Lin, C.R. Wang, and H.L. Li A real-time PCR for SARS-coronavirus incorporating target gene pre-amplification Biochem Biophys Res Commun 312 2003 1290 1296
14. L.L. Poon, K.H. Chan, O.K. Wong, T.K. Cheung, I. Ng, and B. Zheng Detection of SARS-coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays Clin Chem 50 2004 67 72
15. P.R. Grant, J.A. Garson, R.S. Tedder, P.K. Chan, J.S. Tam, and J.J. Sung Detection of SARS-coronavirus in plasma by real-time RT-PCR N Engl J Med 349 2003 2468 2469
16. L.F. Ng, M. Wong, S. Koh, E.E. Ooi, K.F. Tang, and H.N. Leong Detection of severe acute respiratory syndrome coronavirus in blood of infected patients J Clin Microbiol 42 2004 347 350
17. J. Zhai, T. Briese, E. Dai, X. Wang, X. Pang, and Z. Du Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003 Emerg Infect Dis 10 2004 300 303
18. S.S. Jiang, T.C. Chen, J.Y. Yang, C.A. Hsiung, I.J. Su, and Y.L. Liu Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction Clin Infect Dis 38 2004 293 296
19. E. Keyaerts, L. Vijgen, P. Maes, J. Neyts, and M. Van Ranst In vitro inhibition of severe acute respiratory syndrome coronavirus by chloroquine Biochem Biophys Res Commun 323 2004 264 268
20. N. Yamamoto, R. Yang, Y. Yoshinaka, S. Amari, T. Nakano, and J. Cinatl HIV protease inhibitor nelfinavir inhibits replication of SARS-associated coronavirus Biochem Biophys Res Commun 318 2004 719 725