Protons inhibit Cl- conductance by direct or allosteric interaction with the GABA-binding site in the rat recombinant α1β2γ2L and α1β2 GABAA receptor

European Journal of Pharmacology

Volumn 528, Issue 1-3, 2005, Pages 1-6

  Wang, Ming De ^a   Rahman, Mozibur ^a   Zhu, Di ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

GABA; GABAA receptor; Pentobarbital; Proton; Xenopus oocyte

Indexed keywords

4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID A RECEPTOR; BICUCULLINE; CHLORIDE ION; PENTOBARBITAL; RECOMBINANT PROTEIN;

ALLOSTERISM; ANIMAL CELL; ARTICLE; BINDING SITE; CHANNEL GATING; CHLORIDE CONDUCTANCE; CONCENTRATION RESPONSE; CONTROLLED STUDY; FEMALE; NONHUMAN; OOCYTE; PH MEASUREMENT; PRIORITY JOURNAL; PROTON TRANSPORT; XENOPUS;

ALLOSTERIC REGULATION; ANIMALS; BICUCULLINE; CHLORIDES; DOSE-RESPONSE RELATIONSHIP, DRUG; GABA ANTAGONISTS; GABA MODULATORS; GAMMA-AMINOBUTYRIC ACID; HYDROGEN-ION CONCENTRATION; ION CHANNEL GATING; MEMBRANE POTENTIALS; OOCYTES; PHENOBARBITAL; PROTONS; RATS; RECEPTORS, GABA-A; RECOMBINANT PROTEINS; TRANSFECTION; XENOPUS LAEVIS;

EID: 28844503861     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejphar.2005.11.001     Document Type: Article

References (20)

1. G.E. Abraham, D. Tulchinsky, and S.G. Korenman Chromatographic purification of estradiol-17 for use in radio-ligand assay Biochem. Med. 3 1970 365 368
2. J. Amin, and D.S. Weiss GABAA receptor needs two homologous domains of the beta-subunit for activation by GABA but not by pentobarbital Nature 366 1993 565 569
3. R.H. Evans Potentiation of the effects of GABA by pentobarbitone Brain Res. 171 1979 113 120
4. H.J. Feng, and R.L. Macdonald Proton modulation of alpha 1 beta 3 delta GABAA receptor channel gating and desensitization J. Neurophysiol. 92 2004 1577 1585
5. N.P. Franks, and W.R. Lieb Molecular and cellular mechanisms of general anaesthesia Nature 367 1994 607 614
6. R.Q. Huang, and G.H. Dillon Effect of extracellular pH on GABA-activated current in rat recombinant receptors and thin hypothalamic slices J. Neurophysiol. 82 1999 1233 1243
7. R.Q. Huang, Z. Chen, and G.H. Dillon Molecular basis for modulation of recombinant alpha1beta2gamma2 GABAA receptors by protons J. Neurophysiol. 92 2004 883 894
8. K. Kaila Ionic basis of GABAA receptor channel function in the nervous system Prog. Neurobiol. 42 1994 489 537
9. E.F. Kirkness, and A.J. Turner The stimulatory effects of secobarbital and pregnanolone on the GABAA receptor can be blocked selectively Eur. J. Pharmacol. 150 1988 385 388
10. B.J. Krishek, and T.G. Smart Proton sensitivity of rat cerebellar granule cell GABAA receptors: dependence on neuronal development J. Physiol. 530 2001 219 233
11. B.J. Krishek, A. Amato, C.N. Connolly, S.J. Moss, and T.G. Smart Proton sensitivity of the GABA(A) receptor is associated with the receptor subunit composition J. Physiol. 492 Pt 2 1996 431 443
12. J.W. Mozrzymas, A. Barberis, K. Mercik, and E.D. Zarnowska Binding sites, singly bound states, and conformation coupling shape GABA-evoked currents J. Neurophysiol. 89 2003 871 883
13. M. Pasternack, S. Smirnov, and K. Kaila Proton modulation of functionally distinct GABAA receptors in acutely isolated pyramidal neurons of rat hippocampus Neuropharmacology 35 1996 1279 1288
14. J.A. Peters, E.F. Kirkness, H. Callachan, J.J. Lambert, and A.J. Turner Modulation of the GABAA receptor by depressant barbiturates and pregnane steroids Br. J. Pharmacol. 94 1988 1257 1269
15. B. Robertson Actions of anaesthetics and avermectin on GABAA chloride channels in mammalian dorsal root ganglion neurones Br. J. Pharmacol. 98 1989 167 176
16. E. Roberts, and M.A. Sherman GABA-the quintessential neurotransmitter: electroneutrality, fidelity, specificity, and a model for the ligand binding site of GABAA receptors Neurochem. Res. 18 1993 365 376
17. T.G. Smart A novel modulatory binding site for zinc on the GABAA receptor complex in cultured rat neurones J. Physiol. 447 1992 587 625
18. R.E. Study, and J.L. Barker Diazepam and (-)-pentobarbital: fluctuation analysis reveals different mechanisms for potentiation of gamma-aminobutyric acid responses in cultured central neurons Proc. Natl. Acad. Sci. U. S. A. 78 1981 7180 7184
19. M.E. Wilkins, A.M. Hosie, and T.G. Smart Identification of a beta subunit TM2 residue mediating proton modulation of GABA type A receptors J. Neurosci. 22 2002 5328 5333
20. J. Zhai, R.W. Peoples, and C. Li Proton inhibition of GABA-activated current in rat primary sensory neurons Pflugers Arch. 435 1998 539 545