A multiplex polymerase chain reaction assay for rapid detection of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium and Campylobacter jejuni in artificially contaminated food samples

Journal of Rapid Methods and Automation in Microbiology

Volumn 13, Issue 3, 2005, Pages 213-223

  Wang, H ^a   Slavik, M F ^a  

^a UNIVERSITY OF ARKANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); BASIDIOMYCOTA; BRASSICA OLERACEA VAR. BOTRYTIS; CAMPYLOBACTER JEJUNI; CITRULLUS LANATUS VAR. LANATUS; ESCHERICHIA COLI; ESCHERICHIA COLI O157-H7; LACTUCA; LISTERIA MONOCYTOGENES; SALMONELLA TYPHIMURIUM; VITACEAE; VITIS SP.;

EID: 28844489459     PISSN: 10603999     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1745-4581.2005.00015.x     Document Type: Conference Paper

References (27)

1. ACKERS, M.L., MAHON, B.E., LEAHY, E., GOODE, B., DAMROW, T., HAYES, P.S., BIBB, W.F., RICE, D.H., BARRETT, T.J., HUTWAGNER, L. ET AL. 1998. An outbreak of Escherichia coli O157:H7 infection associated with leaf lettuce consumption. J. Infect. Dis. 177, 1588-1593.
2. ALTEKRUSE, S.F., COHEN, M.L. and SWERDLOW, D.L. 1997. Emerging food borne disease. Emerg. Infect. Dis. 3, 285-293.
3. BESNARD, V., FEDERIGHI, M. and CAPPELIER, J.M. 2000. Development of direct viable count procedure for the investigation of VBNC state in Listeria monocytogenes. Lett. Appl. Microbiol. 31, 77-81.
4. BHADURI, S. and COTTRELL, B. 2001. Sample preparation methods for PCR detection of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on beef chuck shoulder using a single enrichment medium. Mol. Cell. Probes 15, 267-274.
5. GILBERT, C., O'LEARY, A., WINTERS, D. and SLAVIK, M. 2003. Development of a multiplex PCR assay for the specific detection of Salmonella, Campylobacter jejuni, Escherichia coli O157:H7, and Listeria monocytogenes. J. Rapid Methods Automat. Microbiol. 11, 61-74.
6. GOLSTEYN, T., KING, R.K., BURCHAK, J. and GANNON, V.P.J. 1991. Sensitive and specific detection of Listeria monocytogenes in milk and ground beef with the polymerase chain reaction. Appl. Environ. Microbiol. 57, 2576-2580.
7. GRANT, M.A. 2003. Evaluation of methods to improve detection of Escherichia coli O157:H7 in fresh produce by multiplex polymerase chain reaction. J. Food Prot. 66(91), 18-24.
8. GUO, X., CHEN, J., BEUCHAT, L.R. and BRACKETT, R.E. 2000. PCR detection of Salmonella enterica serotype Montevideo in and on raw tomatoes using primers derived from hilA. Appl. Environ. Microbiol. 66, 5248-5252.
9. HOLKO, I., URBANOVA, J., KANTIKOVA, M., PASTOROVA, K. and KMEI, V. 2002. PCR detection of Listeria monocytogenes in milk and milk products and differentiation of suspect isolates. ACTA Vet. Brno 71, 125-131.
10. KAWASAKI, S., HORIKOSHI, N., OKADA, Y., TAKESHITA, K., SAMESHIMA, T. and KAWAMOTO, S. 2005. Multiplex PCR for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in meat samples. J. Food Prot. 68, 551-556.
11. LANG, A.L., TSAI, Y.L., MAYER, C.L., PATTON, K.C. and PALMER, C.J. 1994. Multiplex PCR for detection of the heat-labile toxin gene and shiga-like toxin I and II genes in Escherichia coli isolated from natural waters. Appl. Environ. Microbiol. 60, 3145-3149.
12. LI, Y. and MUSTAPHA, A. 2004. Simultaneous detection of Escherichia coli O157:H7, Salmonella, and Shigella in apple cider and produce by a multiplex PCR. J. Food Prot. 67(1), 27-33.
13. MAHON, J., MURPHY, C.K., JONES, P.W. and BARROW, P.A. 1994. Comparison of multiplex PCR and standard bacteriological methods of detecting Salmonella on chicken skin. Lett. Appl. Microbiol. 10, 255-262.
14. NAM, H.M., MURINDA, S.E., NGUYEM, L.T. and OLIVER, S.P. 2004. Evaluation of universal pre-enrichment broth for isolation of Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes from dairy farm environmental samples. Foodborne Pathog. Dis. 1(1), 37-44.
15. NG, L.K., KINGOMBE, C.I.B., YAN, W., TAYLOR, D.E., HIRATSUKA, K., MALIK, N. and GARCIA, M.M. 1997. Specific detection and confirmation of Campylobacter jejuni by DNA hybridization and PCR. Appl. Environ. Microbiol. 11, 4558-4563.
16. RAVEL, J., KNIGHT, I.T., MONOHAN, C.E., HILL, R.T. and COLWELL, R.R. 1995. Temperature-induced recovery of vibrion cholerae from the viable but nonculturable state: Growth or resuscitation? Microbiology 141, 377-383.
17. ROLLINS, D.M. and COLWELL, R.R. 1986. Viable but nonculturable state of Campylobacter jejuni and its role in survival in the natural aquatic environment. Appl. Environ. Microbiol. 9, 531-538.
18. SHEARER, A.E.H., STRAPP, C.M. and JOERGER, R.D. 2001. Evaluation of a polymerase chain reaction-based system for detection of Salmonella enteritidis, Escherichia coli O157:H7, Listeria spp., and Listeria monocytogenes on fresh fruits and vegetables. J. Food Prot. 64, 788-795.
19. TADA, J., OHASHI, T., NISHIMURA, N., OZAKI, H., FUKUSHIMA, S., TAKANO, J., NISHIBUCHI, M. and TAKEDA, Y. 1992. Non-isotopic microtitre plate-based assay for detecting products of polymerase chain reaction amplification: Application to detection of the tdh gene of Vibrio parahaemolyticus. Mol. Cell. Probes 6, 489-494.
20. TODD, E.C. 1997. Epidemiology of foodborne diseases: A worldwide review. World Health Stat. Q 50, 30-50.
21. WALLER, D.F. and OGATA, S.A. 2000. Quantitative immunocapture PCR assay for detection of Campylobacter jejuni in food. Appl. Environ. Microbiol. 9, 4115-4118.
22. WANG, R.F., SLAVIK, M.F. and CAO, W.W. 1992. A rapid PCR method for detection of low numbers of Campylobacter jejuni. J. Rapid Methods Automat. Microbiol. 1, 101-108.
23. WANG, H., FARBER, J.M., MALIK, N. and SANDERS, G. 1999. Improved PCR detection of Campylobacter jejuni from chicken rinses by a simple sample preparation procedure. Int. J. Food Microbiol. 52, 39-45.
24. WEGMULLER, B., LUTHY, J. and CANDRIAN, U. 1993. Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products. Appl. Environ. Microbiol. 7, 2161-2165.
25. WINTERS, D.K., O'LEARY, A.E. and SLAVIK, M.F. 1997. Rapid PCR with nested primers for direct detection of Campylobacter jejuni in chicken washes. Mol. Cell. Probes 11, 267-171.
26. WINTERS, D.K., MALONEY, T.P. and JOHNSON, M.G. 1999. Rapid detection of Listeria monocytogenes by a PCR assay specific for an aminopeptidase. Mol. Cell. Probes 13, 127-131.
27. ZHAO, C., GE, B., VILLENA, J.D., SUDLER, R., YEH, E., ZHAO, S., WHITE, D.G., WAGNER, D. and MENG, J. 2001. Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the greater Washington, DC area. J. Appl. Environ. Microbiol. 12, 5431-5436.