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Volumn 6, Issue 6, 2005, Pages 3243-3251

Reactive thin polymer films as platforms for the immobilization of biomolecules

Author keywords

[No Author keywords available]

Indexed keywords

AMINES; ELLIPSOMETRY; FOURIER TRANSFORM INFRARED SPECTROSCOPY; MONOLAYERS; POLYETHYLENE GLYCOLS; REACTION KINETICS; SELF ASSEMBLY; SPIN COATING; SURFACE PLASMON RESONANCE; SURFACE REACTIONS; THIN FILMS; X RAY PHOTOELECTRON SPECTROSCOPY;

EID: 28844435339     PISSN: 15257797     EISSN: None     Source Type: Journal    
DOI: 10.1021/bm050247u     Document Type: Article
Times cited : (57)

References (60)
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    • and references herein
    • Gong, P.; Grainger, D. W. Surf. Sci. 2004, 570, 67 and references herein.
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    • Dextran gel, a hydrogel system that can provide high affinity binding sites, has been widely investigated as well. It has been shown that the amount of immobilization of proteins on dextran hygrogel is much higher (factor of ∼2) than on typical monolayers due to the higher immobilization capacity of dextran gel
    • Dextran gel, a hydrogel system that can provide high affinity binding sites, has been widely investigated as well. It has been shown that the amount of immobilization of proteins on dextran hygrogel is much higher (factor of ∼2) than on typical monolayers due to the higher immobilization capacity of dextran gel. ((a) Sigal, G. B.; Bamdad, C.; Barberis, A.; Strominger, J.; Whitesides, G. M. Anal. Chem. 1996, 68, 490.
    • (1996) Anal. Chem. , vol.68 , pp. 490
    • Sigal, G.B.1    Bamdad, C.2    Barberis, A.3    Strominger, J.4    Whitesides, G.M.5
  • 21
    • 1342283649 scopus 로고    scopus 로고
    • However, the potential drawback for dextran gels is that it can increase diffusion lengths. For selected biosensor detection schemes the pronounced swelling may be disadvantageous
    • However, the potential drawback for dextran gels is that it can increase diffusion lengths (Toomey, R.; Freidank, D.; Rohe, J. Macromolecules 2004, 37, 882). For selected biosensor detection schemes the pronounced swelling may be disadvantageous.
    • (2004) Macromolecules , vol.37 , pp. 882
    • Toomey, R.1    Freidank, D.2    Rohe, J.3
  • 44
    • 5144219996 scopus 로고    scopus 로고
    • Surface plasmon resonance fluorescence spectroscopy (SPFS) and its applicability in surface and interface analysis have been reviewed recently The evanescent field of surface plasmons, excited at a metal/dielectric interface by a monochromatic light beam (He-Ne laser), is used to excite surface-immobilized fluorophores. The fluorescence emission of the surface-bound dye molecules excited by the amplified electromagnetic field leads to an intensity enhancement. Compared to conventional (label-free) SPR, the sensitivity and specificity of the binding assays are thus increased
    • Surface plasmon resonance fluorescence spectroscopy (SPFS) and its applicability in surface and interface analysis have been reviewed recently (Knoll, W.; Park, H.; Sinner, E. K.; Yao, D. F.; Yu, F. Surf. Sci. 2004, 570, 30).The evanescent field of surface plasmons, excited at a metal/dielectric interface by a monochromatic light beam (He-Ne laser), is used to excite surface-immobilized fluorophores. The fluorescence emission of the surface-bound dye molecules excited by the amplified electromagnetic field leads to an intensity enhancement. Compared to conventional (label-free) SPR, the sensitivity and specificity of the binding assays are thus increased.
    • (2004) Surf. Sci. , vol.570 , pp. 30
    • Knoll, W.1    Park, H.2    Sinner, E.K.3    Yao, D.F.4    Yu, F.5
  • 48
    • 0037465384 scopus 로고    scopus 로고
    • 2 wagging band), which can be exclusively attributed to the crystallized fraction of PEG, is negligible for the film after PEG coupling
    • 2 wagging band), which can be exclusively attributed to the crystallized fraction of PEG, is negligible for the film after PEG coupling (Schönherr, H.; Frank, C.W. Macromolecules 2003, 36, 1188).
    • (2003) Macromolecules , vol.36 , pp. 1188
    • Schönherr, H.1    Frank, C.W.2
  • 49
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    • note
    • For this analysis we excluded data with PEG coverage >90% as these introduce considerable error in the analysis owing to the large uncertainties of the corresponding values.
  • 54
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    • note
    • The theoretical C/N ratio of PNHSMA is 8, while for a 100% converted PNHSMA one can calculate a C/N ratio of 22.
  • 55
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    • note
    • -1) is Avogadro's number, and M is the molecular mass (183 g/mol) of monomeric PNHSMA.
  • 56
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    • note
    • 25
  • 57
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    • Ph.D. Thesis, Johannes-Gutenberg-Universität Mainz, Germany
    • Zizlsperger, M. Ph.D. Thesis, Johannes-Gutenberg-Universität Mainz, Germany, 1998.
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    • Zizlsperger, M.1
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    • note
    • The very slow decrease of the signal with time can be attributed to the dehybridization process of this short 25-mer DNA duplex.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.