Development of real-time PCR (TaqMan®) assays for the detection and quantification of Botrytis cinerea in planta

Plant Physiology and Biochemistry

Volumn 43, Issue 9, 2005, Pages 890-899

  Suarez, M Belen ^a   Walsh, Kathryn ^a   Boonham, Neil ^a   O'Neill, Tim ^b   Pearson, Simon ^c   Barker, Ian ^a  

^a CENTRAL SCIENCE LABORATORY   (United Kingdom)

^b ADAS BOXWORTH   (United Kingdom)

^c Marks & Spencer   (United Kingdom)

Author keywords

Botrytis cinerea; Latency; Quantification; Real time PCR; TaqMan

Indexed keywords

FUNGAL DNA; TUBULIN;

ARTICLE; BOTRYTIS; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLANT LEAF; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE;

BASE SEQUENCE; BOTRYTIS; DNA, FUNGAL; MOLECULAR SEQUENCE DATA; PLANT LEAVES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, NUCLEIC ACID; SPECIES SPECIFICITY; TUBULIN;

BACTERIA (MICROORGANISMS); BOTRYOTINIA FUCKELIANA;

EID: 28444484266     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2005.07.003     Document Type: Article

References (41)

1. S.E. Barnes, and M.W. Shaw Factors affecting symptom production by latent Botrytis cinerea in Primula x polyantha Plant Pathol. 51 2002 746 754
2. R.E. Beever, and P.L. Weeds Taxonomy and genetic variation of Botrytis and Botryiotinia Y. Elad B. Williamson P. Tudzynsky N. Delen Botrytis: Biology, Pathology and Control 2004 Kluwer Academic Pulishers London 29 52
3. N. Boonham, L.G. Perez, M.S. Mendez, E.L. Peralta, A. Blockley, K. Walsh, I. Barker, and R.A. Mumford Development of a real-time RT-PCR assay for the detection of Potato spindle tuber viroid J. Virol. Methods 116 2004 139 146
4. P.G. Braun, and J.C. Sutton Infection cycles and population dynamics of Botrytis cinerea in strawberry leaves Can. J. Plant Pathol. 133 1988 133 141
5. M. Bruwer, B. Lievens, W. Van Hemelrijck, G. Van den Ackerveken, B.P.A. Cammue, and B.P.H.J. Thomma Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR FEMS Microbiol. Lett. 228 2003 241 248
6. J.R. Coley-Smith, K. Verhoeff, and W.R. Jarvis The Biology of Botrytis 1995 Academic Press NewYork
7. M.L. Daughtrey, R.L. Wick, and J.L. Paterson Botrytis blight of flowering potted plants Compendium of Flowering Potted Plant Diseases 1995 American Phytopathological Society Press 11 14
8. M. Filion, S. St-Arnaud, and H. Jabaji-Hare Direct quantification of fungal DNA from soil substrate using real-time PCR J. Microbiol. Methods 53 2003 67 76
9. C. Gachon, and P. Saindrenan Real-time PCR monitoring of fungal development in Arabidopsis thaliana infected by Alternaria brassicola and Botrytis cinerea Plant Physiol. Biochem. 42 2004 367 371
10. X. Gao, T.A. Jackson, K.N. Lambert, S. Li, G.L. Hartman, and T.L. Niblack Detection and quantification of Fusarium solani f. sp glycines in soybean roots with real-time quantitative polymerase chain reaction Plant Dis. 88 2004 1372 1380
11. W.R. Jarvis Latent infections in the pre- and postharvest environment HortScience 29 1994 749 751
12. Z. Kasza, C. Vagvolgyi, M. Fevre, and P. Cotton Molecular characterization and in planta detection of Sclerotinia sclerotiorum endopolygalacturonase genes Curr. Microbiol. 48 2004 208 213
13. R. Kennedy, A.J. Wakeham, K.G. Byrne, U.M. Meyer, and F.M. Dewey A new method to monitor airborne inoculum of the fungal plant pathogens Mycosphaerella brassicicola and Botrytis cinerea Appl. Environ. Microbiol. 66 2000 2996 3003
14. S.L. de Kock, and G. Holz Blossom-end rot of pears: systemic infection of flowers and immature fruit by Botrytis cinerea J. Phytopathol. 135 1992 317 327
15. G. Lavy-Mair, R. Barkai-Golan, and E. Kopeliovitch Initiation at the stage of postharvest Botrytis stem-end rot in normal and non-ripening fruits Ann. Appl. Biol. 112 1988 393 396
16. K.J. Livak, S.P.A. Flood, J. Marmejo, W. Giusti, and K. Deetz Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR products and nucleic acid hybridization PCR Methods Appl. 4 1995 357 362
17. M.M. Lopez, E. Bertolini, A. Olmos, P. Caruso, M.T. Gorris, P. Llop, R. Penyalver, and M. Cambra Innovative tools for detection of plant pathogenic viruses and bacteria Int. Microbiol. 6 2003 233 243
18. S. Mathur, and R. Utkhede Development of a dot blot technique for rapid identification of Botrytis cinerea, the causal organism of grey mould in greenhouse tomatoes J. Hortic. Sci. Biotechnol. 77 2002 604 608
19. H.A. McCartney, S.J. Foster, B.A. Fraaije, and E. Ward Molecular diagnostics for fungal plant pathogens Pest Manag. Sci. 59 2003 129 142
20. R. McNicol, and B. Williamson Systemic infection of black currant flowers by Botritis cinerea and its possible involvement in premature abscission of fruits Ann. Appl. Biol. 114 1989 243 254
21. R.A. Mumford, K. Walsh, I. Barker, and N. Boonham Detection of Potato mop top virus and Tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay Virology 90 2000 448 453
22. N.G. Nair, and R.N. Allen Infection of grape flowers and berries by Botrytis cinerea as a function of time and temperature Mycol. Res. 97 1993 1012 1014
23. M.P. Pantou, A. Mavridou, and M.A. Typas IGS sequence variation, group-I introns and te complete nuclear ribosomal DNA of the entomopathogenic fungus Metarhizium: excellent tools for isolate detection and phylogenetic analysis Fungal Genet. Biol. 38 2003 159 174
24. P. Pramateftaki, P. Antoniou, and M.A. Typas The complete rDNA sequence of the nuclear ribosomal RNA gene complex of Verticillium dahliae: intraspecific heterogeneity within the intergenic spacer region Fungal Genet. Biol. 29 2000 134 143
25. C. Ratti, G. Budge, L. Ward, G. Clover, C. Rubies-Autonell, and C. Henry Detection and relative quantification of Soil-borne cereal mosaic virus (SBCMV) and Polymyxa graminis in winter wheat using real-time PCR (TaqMan (R)) J. Virol. Methods 122 2004 95 103
26. S. Rigotti, K. Gindro, H. Richter, and O. Viret Characterization for molecular markers for specific and sensitive detection of Botrytis cinerea Pers.: Fr. In strawberry (Fragaria x ananassa) using PCR FEMS Microbiol. 209 2002 169 174
27. N.W. Schaad, and R.D. Frederick Real-time PCR and its application for rapid plant disease diagnostics Can. J. Plant Pathol. 24 2002 250 258
28. M. Sosa-Alvarez, L.V. Madden, and M.A. Ellis Effects of temperature and wetness duration on sporulation of Botrytis cinerea on strawberry leaf residues Plant Dis. 79 1995 609 615
29. M. Staats, P. van Baarlen, and J.A.L. van Kan Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity Mol. Biol. Evol. 22 2005 333 346
30. J. Stenman, and A. Orpana Accuracy in amplification Nat. Biotechnol. 19 2001 1011 1012
31. J.C. Sutton Epidemiology and management of Botrytis leaf-blight of onion and gray mold of strawberry - a comparative-analysis Can. J. Plant Pathol. 12 1990 282 285
32. J.R. Thompson, and B.A. Latorre Characterization of Botrytis cinerea from table grapes in Chile using RAPD-PCR Plant Dis. 83 1999 1090 1094
33. M. Walter, K.S.H. Boyd-Wilson, J.H. Perry, P.A.G. Elmer, and C.M. Frampton Survival of Botrytis cinerea conidia on kiwifruit Plant Pathol. 48 1999 823 829
34. L.I. Ward, P.A. Beales, A.V. Barnes, and C.R. Lane A real-time PCR assay based method for routine diagnosis of Spongospora subterranea on potato tubers J. Phytopathol. 152 2004 633 638
35. L.I. Ward, M.G.E. Fenn, and C.M. Henry A rapid method for direct detection of Polymyxa DNA in soil Plant Pathol. 53 2004 485 490
36. E. Ward, S.J. Foster, B.A. Fraaije, and H.A. McCartney Plant pathogen diagnostics: immunological and nucleic acid-based approaches Ann. Appli. Biol. 145 2004 1 16
37. S.A. Weller, J.G. Elphinstone, N.C. Smith, N. Boonham, and D.E. Stead Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay Appl. Environ. Microbiol. 66 2000 2853 2858
38. S.A. Weller, S.A. Simpkins, D.E. Stead, A. Kurdziel, H. Hird, and R.J. Weekes Identification of Agrobacterium spp. present within Brassica napus seed by TaqMan PCR - Implications for GM screening procedures Arch. Microbiol. 178 2002 338 343
39. S.A. Weller, and D.E. Stead Detection of root mat associated Agrobacterium strains from plant material and other sample types by post-enrichment TaqMan PCR J. Appl. Microbiol. 92 2002 118 126
40. T.J. White, T. Bruns, S. Lee, and J. Tailor Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics M.A. Innis D.H. Gelfand J.J. Sninsky T.J. White PCR Protocols. A Guide to Methods and Applications 1990 Academic Press, Inc New York 315 322
41. C.T. Wittwer, M.G. Herrmann, A.A. Moss, and A.P. Rasmussen Continuous fluorescence monitoring of rapid cycle DNA amplification Biotechniques 22 1997 130 138