The 3.4-kDa Ost4 protein is required for the assembly of two distinct oligosaccharyltransferase complexes in yeast

Glycobiology

Volumn 15, Issue 12, 2005, Pages 1396-1406

  Spirig, Urs ^a   Bodmer, Daniel ^a   Wacker, Michael ^a   Burda, Patricie ^a   Aebi, Markus ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Blue native gel electrophoresis; N linked protein glycosylation; Oligosaccharyltransferase; Saccharomyces cerevisiae

Indexed keywords

ASPARAGINE; CELL PROTEIN; LIPID; OLIGOSACCHARIDE; OLIGOSACCHARYLTRANSFERASE; POLYPEPTIDE; PROTEIN ANTIBODY; PROTEIN OST1P; PROTEIN OST3P; PROTEIN OST4; PROTEIN OST4P; PROTEIN OST6P; PROTEIN STT3P; PROTEIN SWP1P; PROTEIN WBP1P; SACCHAROMYCES CEREVISIAE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME MECHANISM; GEL ELECTROPHORESIS; GLYCOSYLATION; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; SACCHAROMYCES CEREVISIAE; WILD TYPE;

CATALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPLASMIC RETICULUM; FUNGAL PROTEINS; GENE DELETION; GLYCOSYLATION; HEXOSYLTRANSFERASES; KINETICS; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; OLIGOSACCHARIDES; PEPTIDES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPERATURE; TIME FACTORS;

SACCHAROMYCES CEREVISIAE;

EID: 28444437028     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/cwj025     Document Type: Article

References (52)

1. Burda, P. and Aebi, M. (1999) The dolichol pathway of N-linked glycosylation. Biochim. Biophys. Acta, 1426, 239-257.
2. Burda, P., te Heesen, S., Brachat, A., Wach, A., Dusterhoft, A., and Aebi, M. (1996) Stepwise assembly of the lipid-linked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: Identification of the ALG9 gene encoding a putative mannosyl transferase. Proc. Natl. Acad. Sci. U. S. A., 93, 7160-7165.
3. Chi, J.H., Roos, J., and Dean, N. (1996) The OST4 gene of saccharomyces cerevisiae encodes an unusually small protein required for normal levels of oligosaccharyltransferase activity. J. Biol. Chem., 271, 3132-3140.
4. Cummings, R.D. (1992) Synthesis of asparagine-linked oligosaccharides: pathways, genetics, and metabolic regulation. In Allen, H.J. and Kisalius, E.C. (eds), Glycoconjugates, Composition, Structure, and Function. Marcel Dekker, New York, pp. 333-360.
5. Das, R.C. and Heath, E.C. (1980) Dolichyldiphosphoryloligosaccharide - protein oligosaccharyltransferase; solubilization, purification, and properties. Proc. Natl. Acad. Sci. U. S. A., 77, 3811-3815.
6. Dekker, P.J., Muller, H., Rassow, J., and Pfanner, N. (1996) Characterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis. Biol. Chem., 377, 535-538.
7. Dekker, P.J., Ryan, M.T., Brix, J., Muller, H., Honlinger, A., and Pfanner, N. (1998) Preprotein translocase of the outer mitochondrial membrane. Molecular dissection and assembly of the general import pore complex. Mol. Cell Biol., 18, 6515-6524.
8. Dempski, R.E. and Imperiali, B. (2002) Oligosaccharyl transferase: gatekeeper to the secretory pathway. Curr. Opin. Chem. Biol., 6, 844-850.
9. Fleischmann, M., Clark, M.W., Forrester, W., Wickens, M., Nishimoto, T., and Aebi, M. (1991) Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation. Mol. Gen. Genet., 227, 417-423.
10. Guthrie, C. and Fink, G.R. (1991) Guide to Yeast Genetics and Molecular Biology. Academic Press, San Diego, CA.
11. Helenius, A. and Aebi, M. (2004) Roles of N-linked glycans in the endoplasmic reticulum. Annu. Rev. Biochem., 73, 1019-1049.
12. Herscovics, A. and Orlean, P. (1993) Glycoprotein biosynthesis in yeast. FASEB J., 7, 540-550.
13. Hiller, M.M., Finger, A., Schweiger, M., and Wolf, D.H. (1996) ER degradation of a misfolded luminal protein by the cytosolic ubiquitin-proteasome pathway. Science, 273, 1725-1728.
14. Jakob, C.A., Burda, P., Roth, J., and Aebi, M. (1998) Degradation of misfolded ER glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure. J. Cell Biol., 142, 1223-1233.
15. Karaoglu, D., Kelleher, D.J., and Gilmore, R. (1995) Functional characterisation of Ost3p. Loss of the 34-kD subunit of the Saccharomyces cerevisiae oligosaccharyltransferase results in biased underglycosylation of acceptor substrates. J. Cell Biol., 130, 567-578.
16. Karaoglu, D., Kelleher, D.J., and Gilmore, R. (1997) The highly conserved Stt3 protein is a subunit of the yeast oligosaccharyltransferase and forms a subcomplex with Ost3p and Ost4p. J. Biol. Chem., 272, 32513-32520.
17. Kelleher, D.J. and Gilmore, R. (1994) The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p, and four additional polypeptides. J. Biol. Chem., 269, 12908-12917.
18. Kelleher, D.J. and Gilmore, R. (1997) DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. U. S. A., 94, 4994-4999.
19. Kelleher, D.J., Kreibich, G., and Gilmore, R. (1992) Oligosaccharyltransferase activity is associated with a protein complex composed of ribophorin I and II and a 48 kd protein. Cell, 69, 55-65.
20. Kelleher, D.J., Karaoglu, D., Mandon, E.C., and Gilmore, R. (2003) Oligosaccharyltransferase isoforms that contain different catalytic STT3 subunits have distinct enzymatic properties. Mol. Cell, 12, 101-111.
21. Kim, H., Yan, Q., Von Heijne, G., Caputo, G.A., and Lennarz, W.J. (2003) Determination of the membrane topology of Ost4p and its subunit interactions in the oligosaccharyltransferase complex in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U. S. A., 100, 7460-7464.
22. Knauer, R. and Lehle, L. (1994) The N-oligosaccharyltransferase complex from yeast. FEBS Lett., 344, 83-86.
23. Knauer, R. and Lehle, L. (1999a) The oligosaccharyltransferase complex from Saccharomyces cerevisiae - isolation of the OST6 gene, its synthetic interaction with OST3, and analysis of the native complex. J. Biol. Chem., 274, 17249-17256.
24. Knauer, R. and Lehle, L. (1999b) The oligosaccharyltransferase complex from yeast. Biochim. Biophys. Acta, 1426, 259-273.
25. Kornfeld, R., Bao, M., Brewer, K., Noll, C., and Canfield, W. (1999) Molecular cloning and functional expression of two splice forms of human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase. J. Biol. Chem., 274, 32778-32785.
26. Li, G., Yan, Q., Oen, H.O., and Lennarz, W.J. (2003) A specific segment of the transmembrane domain of Wbp1p is essential for its incorporation into the oligosaccharyl transferase complex. Biochemistry, 42, 11032-11039.
27. Nilsson, I., Kelleher, D.J., Miao, Y., Shao, Y., Kreibich, G., Gilmore, R., von Heijne, G., and Johnson, A.E. (2003) Photocross-linking of nascent chains to the STT3 subunit of the oligosaccharyltransferase complex. J. Cell Biol., 161, 715-725.
28. Pathak, R. and Imperiali, B. (1997) A dual affinity tag on the 64-kDa Nlt1p subunit allows the rapid characterization of mutant yeast oligosaccharyl transferase complexes. Arch. Biochem. Biophys., 338, 1-6.
29. Reiss, G., te Heesen, S., Gilmore, R., Zufferey, R., and Aebi, M. (1997) A specific screen for oligosaccharyltransferase mutations identifies the 9 kDa OST5 protein required for optimal activity in vivo and in vitro. EMBO J., 16, 1164-1172.
30. Sailer, A. and Weissmann, C. (1991) A sensitive and rapid protein assay impervious to detergents. Technique, 3, 37-38.
31. Schägger, H. (1995) Native electrophoresis for isolation of mitochondrial oxidative phosphorylation protein complexes. Methods Enzymol., 260, 190-202.
32. Schägger, H. and von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem., 199, 223-231.
33. Schägger, H., Cramer, W.A., and von Jagow, G. (1994) Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem., 217, 220-230.
34. Shibatani, T., David, L.L., McCormack, A.L., Frueh, K., and Skach, W.R. (2005) Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits. Biochemistry, 44, 5982-5992.
35. Silberstein, S. and Gilmore, R. (1996) Biochemistry, molecular biology, and genetics of the oligosaccharyltransferase. FASEB J., 10, 849-858.
36. Silberstein, S., Kelleher, D.J., and Gilmore, R. (1992) The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. J. Biol. Chem., 267, 23658-23663.
37. Silberstein, S., Collins, P.G., Kelleher, D.J., and Gilmore, R. (1995a) The essential OST2 gene encodes the 16-kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms. J. Cell Biol., 131, 371-383.
38. Silberstein, S., Collins, P.G., Kelleher, D.J., Rapiejko, P.J., and Gilmore, R. (1995b) The alpha subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex I essential for vegetative growth of yeast and is homologous to mammalian ribophorin I. J. Cell Biol., 128, 525-536.
39. Spirig, U., Glavas, M., Bodmer, D., Reiss, G., Burda, P., Lippuner, V., te Heesen, S., and Aebi, M. (1997) The STT3 protein is a component of the yeast oligosaccharyltransferase complex. Mol. Gen. Genet., 256, 628-637.
40. Spiro, R.G., Spiro, M.J., and Bhoyroo, V.D. (1976) Lipid-saccharide intermediates in glycoprotein biosynthesis. II. Studies on the structure of an oligosaccharide-lipid from thyroid. J. Biol. Chem., 251, 6409-6419.
41. Stagljar, I., te Heesen, S., and Aebi, M. (1994) New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: Cloning of the ALG8 locus. Proc. Natl. Acad. Sci. U. S. A., 91, 5977-5981.
42. Tanner, W. and Lehle, L. (1987) Protein glycosylation in yeast. Biochim. Biophys. Acta, 906, 81-99.
43. te Heesen, S., Janetzky, B., Lehle, L., and Aebi, M. (1992) The yeast WBP1 is essential for oligosaccharyltransferase activity in vivo and in vitro. EMBO J., 11, 2071-2075.
44. te Heesen, S., Knauer, R., Lehle, L., and Aebi, M. (1993) Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyl transferase activity. EMBO J., 12, 279-284.
45. Vijayraghavan, U., Company, M., and Abelson, J. (1989) Isolation and characterisation of pre-mRNA splicing mutants in Saccharomyces cerevisiae. Genes Dev., 3, 1206-1216.
46. Wacker, M., Linton, D., Hitchen, P.G., Nita-Lazar, M., Haslam, S.M., North, S.J., Panico, M., Morris, H.R., Dell, A., Wren, B.W., and Aebi, M. (2002) N-linked glycosylation in Campylobacter jejuni and its functional transfer into E-coli. Science, 298, 1790-1793.
47. Wieland, F.T., Gleason, M.L., Serafini, T.A., and Rothman, J.E. (1987) The rate of bulk flow from the endoplasmic reticulum to the cell surface. Cell, 50, 289-300.
48. Wilson, C.M., Kraft, C., Duggan, C., Ismail, N., Crawshaw, S.G., and High, S. (2005) Ribophorin I associates with a subset of membrane proteins after their integration at the Sec61 translocon. J. Biol. Chem., 280, 4195-4206.
49. Yan, G. and Lennarz, W.J. (2002) Studies on the function of oligosaccharyl transferase subunits - Stt3p is directly involved in the glycosylation process. J. Biol. Chem., 277, 47692-47700.
50. Yan, A.X. and Lennarz, W.J. (2005a) Unraveling the mechanism of protein N-glycosylation. J. Biol. Chem., 280, 3121-3124.
51. Yan, A.X. and Lennarz, W.J. (2005b) Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons. Glycobiology, 15.
52. Zufferey, R., Knauer, R., Burda, P., Stagljar, I., te Heesen, S., Lehle, L., and Aebi, M. (1995) STT3, a highly conserved protein required for yeast oligosaccharyl transferase activity in vivo. EMBO J., 14, 4949-4960.