Preprotein translocase of the outer mitochondrial membrane: Molecular dissection and assembly of the general import pore complex

Molecular and Cellular Biology

Volumn 18, Issue 11, 1998, Pages 6515-6524

  Dekker, Peter J T ^a   Ryan, Michael T ^a   Brix, Jan ^a   Müller, Hanne ^a   Hönlinger, Angelika ^a   Pfanner, Nikolaus ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; PROTEIN PRECURSOR;

ARTICLE; CELL MEMBRANE TRANSPORT; COMPLEX FORMATION; GEL ELECTROPHORESIS; IMMUNOPRECIPITATION; MITOCHONDRIAL MEMBRANE; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN STRUCTURE;

EID: 0031741738     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.18.11.6515     Document Type: Article

References (56)

1. Alconada, A., F. Gärtner, A. Hönlinger, M. Kübrich, and N. Pfanner. 1995. Mitochondrial receptor complex from Neurospora crassa and Saccharomyces cerevisiae. Methods Enzymol. 260:263-286.
2. Alconada, A., M. Kübrich, M. Moczko, A. Hönlinger, and N. Pfanner. 1995. The mitochondnal receptor complex: the small subunit Mom8b/Isp6 supports association of receptors with the general insertion pore and transfer of preproteins. Mol. Cell. Biol. 15:6196-6205.
3. Blom, J., P. J. T. Dekker, and M. Meijer. 1995. Functional and physical interactions of components of the yeast mitochondrial inner-membrane import machinery (MIM). Eur. J. Biochem. 232:309-314.
4. Bolliger, L., T. Junne, G. Schatz, and T. Lithgow. 1995. Acidic receptor domains on both sides of the outer membrane mediate translocation of precursor proteins into yeast mitochondria. EMBO J. 14:6318-6326.
5. Bömer, U., N. Pfanner, and K. Dietmeier. 1996. Identification of a third yeast mitochondrial Tom protein with tetratrico peptide repeats. FEBS Lett. 382: 153-158.
6. Brix, J., K. Dietmeier, and N. Pfanner. 1997. Differential recognition of preproteins by the purified cytosolic domains of the mitochondrial import receptors Tom20, Tom22, and Tom70. J. Biol. Chem. 272:20730-20735.
7. Court, D. A., F. E. Nargang, H. Steiner, R. S. Hodges, W. Neupert, and R. Lill. 1996. Role of the intermembrane space domain of the preprotein receptor Tom22 in protein import into mitochondria. Mol. Cell. Biol. 16: 4035-4042.
8. 2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J. Biol. Chem. 257:13028-13033.
9. Dekker, P. J. T., F. Martin, A. C. Maarse, U. Bömer, H. Müller, B. Guiard, M. Meijer, J. Rassow, and N. Pfanner. 1997. The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44. EMBO J. 16: 5408-5419.
10. Dekker, P. J. T., H. Müller, J. Rassow, and N. Pfanner. 1996. Characterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis. Biol. Chem. 377:535-538.
11. Dietmeier, K., A. Hönlinger, U. Bömer, P. J. T. Dekker, C. Eckerskorn, F. Lottspeich, M. Kübrich, and N. Pfanner. 1997. Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature 388: 195-200.
12. Gratzer, S., T. Lithgow, R. E. Bauer, E. Lamping, F. Paltauf, S. D. Kohlwein, V. Haucke, T. Junne, G. Schatz, and M. Horst. 1995. Mas37p, a novel receptor subunit for protein import into mitochondria. J. Cell Biol. 129:25-34.
13. Harkness, T. A., F. E. Nargang, I. van der Klei, W. Neupert, and R. Lill. 1994. A crucial role of the mitochondrial protein import receptor MOM19 for the biogenesis of mitochondria. J. Cell Biol. 124:637-648.
14. Hartl, F. U., J. Ostermann, B. Guiard, and W. Neupert. 1987. Successive translocation into and out of the mitochondrial matrix: targeting of proteins to the intermembrane space by a bipartite signal peptide. Cell 51:1027-1037.
15. Haucke, V., M. Horst, G. Schatz, and T. Lithgow. 1996. The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor. EMBO J. 15:1231-1237.
16. Hönlinger, A., U. Bömer, A. Alconada, C. Eckerskorn, F. Lottspeich, K. Dietmeier, and N. Pfanner. 1996. Tom7 modulates the dynamics of the mitochondrial outer membrane translocase and plays a pathway-related role in protein import. EMBO J. 15:2125-2137.
17. Hönlinger, A., P. Keil, R. J. Nelson, E. A. Craig, and N. Pfanner. 1995 Posttranslational mitochondrial protein import in a homologous yeast in vitro system. Biol. Chem. 376:515-519.
18. Hönlinger, A., M. Kübrich, M. Moczko, F. Gärtner, L. Mallet, F. Bussereau, C. Eckerskorn, F. Lottspeich, K. Dietmeier, M. Jacquet, and N. Pfanner. 1995. The mitochondrial receptor complex: Mom22 is essential for cell viability and directly interacts with preproteins. Mol. Cell. Biol. 15:3382-3389.
19. Horst, M., S. Hilfiker-Rothenfluh, W. Oppliger, and G. Schatz. 1995. Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria. EMBO J. 14:2293-2297.
20. Hwang, S., T. Jascur, D. Vestweber, L. Pon, and G. Schatz. 1989. Disrupted yeast mitochondria can import precursor proteins directly through their inner membrane. J. Cell Biol. 109:487-493.
21. Kassenbrock, C. K., W. Cao, and M. G. Douglas. 1993. Genetic and biochemical characterization of ISP6, a small mitochondrial outer membrane protein associated with the protein translocation complex. EMBO J. 8:3023-3034.
22. Keil, P., and N. Pfanner. 1993. Insertion of MOM22 into the mitochondrial outer membrane strictly depends on surface receptors. FEBS Lett. 321:197-200.
23. Keil, P., A. Weinzierl, M. Kiebler, K. Dietmeier, T. Söllner, and N. Pfanner. 1993. Biogenesis of the mitochondrial receptor complex: two receptors are required for binding of MOM38 to the outer membrane surface. J. Biol. Chem. 268:19177-19180.
24. Kiebler, M., P. Keil, H. Schneider, I. J. van der Klei, N. Pfanner, and W. Neupert. 1993. The mitochondrial receptor complex: a central role of MOM22 in mediating preprotein transfer from receptors to the general insertion pore. Cell 74:483-492.
25. Kiebler, M., R. Pfaller, T. Söllner, G. Griffiths, H. Horstman, N. Pfanner, and W. Neupert. 1990. Identification of a mitochondrial receptor complex required for recognition and membrane insertion of precursor proteins. Nature 348:610-616.
26. Komiya, T., S. Rospert, G. Schatz, and K. Mihara. 1997. Binding of mitochondrial precursor proteins to the cytoplasmic domains of the import receptors Tom70 and Tom20 is determined by cytoplasmic chaperones. EMBO J. 16:4267-4275.
27. Komiya, T., M. Sakaguchi, and K. Mihara. 1996. Cytoplasmic chaperones determine the targeting pathway of precursor proteins to mitochondria. EMBO J. 15:399-407.
28. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
29. Lithgow, T., B. S. Glick, and G. Schatz. 1995. The protein import receptor of mitochondria. Trends Biochem. Sci. 20:98-101.
30. Lithgow, T., T. Junne, K. Suda, S. Gratzer, and G. Schatz. 1994. The mitochondrial outer membrane protein Mas22p is essential for protein import and viability of yeast. Proc. Natl. Acad. Sci. USA 91:11973-11977.
31. Lithgow, T., T. Junne, C. Wachter, and G. Schatz. 1994. Yeast mitochondria lacking the two import receptors Mas20p and Mas70p can efficiently and specifically import precursor proteins. J. Biol. Chem. 269:15325-15330.
32. Mayer, A., F. E. Nargang, W. Neupert, and R. Lill. 1995. MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19. EMBO J. 14:4204-4211.
33. Moczko, M., U. Bömer, M. Kübrich, N. Zufall, A. Hönlinger, and N. Pfanner. 1997. The intermembrane space domain of mitochondrial Tom22 functions as a trans binding site for preproteins with N-terminal targeting sequences. Mol. Cell. Biol. 17:6574-6584.
34. Moczko, M., K. Dietmeier, T. Söllner, B. Segui, H. F. Steger, W. Neupert, and N. Pfanner. 1992. Identification of the mitochondrial receptor complex in Saccharomyces cerevisiae. FEBS Lett. 10:265-268.
35. Nakai, M., K. Kinoshita, and T. Endo. 1995. Mitochondrial receptor complex protein. The intermembrane space domain of yeast MAS17 is not essential for its targeting or function. J. Biol. Chem. 270:30571-30575.
36. Neupert, W. 1997. Protein import into mitochondria. Annu. Rev. Biochem. 66:863-917.
37. Pfanner, N., E. A. Craig, and A. Hönlinger. 1997. Mitochondrial preprotein translocase. Annu. Rev, Cell Dev. Biol. 13:25-51.
38. Pfanner, N., M. G. Douglas, T. Endo, N. J. Hoogenraad, R. E. Jensen, M. Meijer, W. Neupert, G. Schatz, U. K. Schmitz, and G. C. Shore. 1996. Uniform nomenclature for the protein transport machinery of the mitochondrial membranes. Trends Biochem. Sci. 21:51-52.
39. Ramage, L., T. Junne, K. Hahne, T. Lithgow, and G. Schatz. 1993. Functional cooperation of mitochondrial protein import receptors in yeast. EMBO J. 12:4115-4123.
40. Rapaport, D., W. Neupert, and R. Lill. 1997. Mitochondrial protein import: Tom40 plays a major role in targeting and translocation of preproteins by forming a specific binding site for the presequence. J. Biol. Chem. 272: 18725-18731.
41. Rassow, J., and N. Pfanner. 1991. Mitochondrial preproteins en route from the outer membrane to the inner membrane are exposed to the intermembrane space. FEBS Lett. 293:85-88.
42. Ryan, K. R., and R. E. Jensen. 1995. Protein translocation across mitochondrial membranes: what a long, strange trip it is. Cell 83:517-519.
43. Ryan, M. T., and N. Pfanner. Unpublished data.
44. Schägger, H., W. A. Cramer, and G. von Jagow. 1994. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem. 217:220-230.
45. Schägger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
46. Schägger, H., and G. von Jagow. 1991. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199:223-231.
47. Schatz, G. 1997. Just follow the acid chain. Nature 388:121-122.
48. Schatz, G., and B. Dobberstein. 1996. Common principles of protein translocation across membranes. Science 271:1519-1526.
49. Schleif, E., G. C. Shore, and I. S. Goping. 1997. Interactions of the human mitochondrial protein import receptor, hTom20, with precursor proteins in vitro reveal pleiotropic specificities and different receptor domain requirements. J. Biol. Chem. 272:17784-17789.
50. Schleyer, M., and W. Neupert. 1985. Transport of proteins into mitochondria: translocational intermediates spanning contact sites between outer and inner membranes. Cell 43:339-350.
51. Schlossmann, J., R. Lill, W. Neupert, and D. A. Court. 1996. Tom71, a novel homologue of the mitochondrial preprotein receptor Tom70. J. Biol. Chem. 271:17890-17895.
52. Segui-Real, B., G. Kispal, R. Lill, and W. Neupert. 1993. Functional independence of the protein translocation machineries in mitochondrial outer and inner membranes: passage of preproteins through the intermembrane space. EMBO J. 12:2211-2218.
53. Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.
54. Söllner, T., J. Rassow, and N. Pfanner. 1991. Analysis of mitochondrial protein import using translocation intermediates and specific antibodies. Methods Cell Biol. 3:345-358.
55. Söllner, T., J. Rassow, M. Wiedmann, J. Schlossmann, P. Keil, W. Neupert, and N. Pfanner. 1992. Mapping of the protein import machinery in the mitochondrial outer membrane by crosslinking of translocation intermediates. Nature 355:84-87.
56. Vestweber, D., J. Brunner, A. Baker, and G. Schatz. 1989. A 42K outer-membrane protein is a component of the yeast mitochondrial protein import site. Nature 341:205-209.