Biochemistry, molecular biology, and genetics of the oligosaccharyltransferase

FASEB Journal

Volumn 10, Issue 8, 1996, Pages 849-858

  Silberstein, Susana ^a   Gilmore, Reid ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

endoplasmic reticulum; hypoglycosylation; N linked glycosylation; yeast OST subunits

Indexed keywords

ASPARAGINE LINKED OLIGOSACCHARIDE; OLIGOSACCHARIDE; OLIGOSACCHARYLTRANSFERASE; TRANSFERASE; UNCLASSIFIED DRUG;

CARBOHYDRATE SYNTHESIS; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBUNIT; MOLECULAR GENETICS; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; PROTEIN MODIFICATION; REVIEW; SEQUENCE HOMOLOGY; YEAST;

EUKARYOTA; INVERTEBRATA; VERTEBRATA;

EID: 0029991763     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fasebj.10.8.8666161     Document Type: Review

References (69)

1. Hirschberg, C. B., and Snider, M. D. (1987) Topography of glycosylation in the rough endoplasmic reticulum and Golgi apparatus. Annu. Rev. Biochem. 56, 63-87
2. Herscovics, A., and Orlean, P. (1993) Glycoprotein biosynthesis in yeast. FASEB J. 7, 540-550
3. Rapoport, T. A. (1992) Transport of proteins across the endoplasmic reticulum membrane. Science 258, 931-936
4. Gilmore, R. (1993) Protein translocation across the endoplasmic reticulum: A tunnel with toll booths at entry and exit. Cell 75, 589-592
5. Walter, P., and Johnson, A. E. (1994) Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu. Rev. Cell Biol. 10, 87-119.
6. Helenius, A. (1994) How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum. Mol. Biol. Cell 5, 253-265
7. Fiedler, K., and Simons, K. (1995) The role of N-glycans in the secretory pathway. Cell 81, 309-312
8. Marshall, R. D. (1972) Glycoproteins. Annu. Rev. Biochem. 41, 673-702
9. Bause, E., and Legler, G. (1981) The role of the hydroxy amino acid in the triplet sequence asn-xaa-thr(ser) for the N-glycosylation step during glycoprotein biosynthesis. Biochem. J. 195, 639-644
10. Bause, E. (1983) Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes. Biochem. J. 209, 331-336
11. Miletich, J. P., and Broze, G. J. J. (1990) β Protein C is not glycosylated at asparagine 329. J. Biol. Chem. 265, 11397-11404
12. Grinnell, B. W., Walls, J. D., and Gerlitz, B. (1991) Glycosylation of human protein C affects its secretion, processing, functional activities, and its activation by thrombin. J. Biol. Chem. 266, 9778-9785
13. Titani, K., Kumar, S., Takio, K., Ericsson, L. H., Wade, R. D., Ashida, K., Walsh, K. A., Chopek, M. W., Sadler, J. E., and Fujikawa, K. (1986) Amino acid sequence of human von Willebrand factor. Biochemistry 25, 3171-3184
14. Gavel, Y., and Von Heijne, G. (1990) Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. Protein Eng. 3, 433-442
15. Imperiali, B., and Shannon, K. L. (1991) Differences between Asn-Xaa-Thr containing peptides: A comparison of solution conformation and substrate behavior with oligosaccharyltransferase. Biochemistry 30, 4374-4380
16. Picard, V., Ersdal-Badju, E., and Bock, S. C. (1995) Partial glycosylation of antithrombin III asparagine-135 is caused by the serine in the third position of its N-glycosylation sequence and is responsible for production of the β-antithrombin III isoform with enhanced heparin affinity. Biochemistry 34, 8433-8440
17. Reddy, V. A., Johnson, R. S., Biemann, K., Williams, R. S., Ziegler, F. D., Trimble, R. B., and Maley, F. (1988) Characterization of the glycosylation sites in yeast external invertase. 1. N-linked oligosaccharide content of the individual sequons. J. Biol. Chem. 263, 6978-6985
18. Nilsson, I., and von Heijne, G. (1993) Determination of the distance between the oligosaccharyltransferase active site and the endoplasmic reticulum membrane. J. Biol. Chem. 268, 5798-5801
19. Shakin-Eshleman, S. H., Wunner, W. H., and Spitalnik, S. L. (1993) Efficiency of N-linked core glycosylation at asparagine-319 of rabies virus glycoprotein is altered by deletions C-terminal to the glycosylation sequon. Biochemistry 32, 9465-9472
20. Pless, D. D., and Lennarz, W. J. (1977) Enzymatic conversion of proteins to glycoproteins. Proc. Natl. Acad. Sci. USA 74, 134-138
21. Jethmalani, S. M., Henle, K. J., and Kaushal, G. P. (1994) Heat shock-induced prompt glycosylation. Identification of P-SC67 as calreticulin. J. Biol. Chem. 269, 23603-23609
22. Allen, S., Naim, H. Y., and Bulleid, N. J. (1995) Intracellular folding of tissue-type plasminogen activator. Effects of disulfide bond formation on N-linked glycosylation and secretion. J. Biol. Chem. 276, 4797-4804
23. McGinnes, L. W., and Morrison, T. G. (1994) The role of individual cysteine residues in the formation of the mature, antigenic HN protein of Newcastle disease virus. Virology 200, 470-483
24. Parodi, A. J. (1993) N-glycosylation in trypanosomatid protozoa. Glycobiology 3, 193-199
25. Sharma, C. B., Lehle, L., and Tanner, W. (1981) N-glycosylation of yeast proteins. Characterization of the solubilized oligosaccharyltransferase. Eur. J. Biochem. 116, 101-108
26. Trimble, R. B., Byrd, J. C., and Maley, F. (1980) Effect of glucosylation of lipid intermediates on oligosaccharide transfer in solubilized microsomes from Saccharomyces cerevisiae. J. Biol. Chem. 255, 11892-11895
27. Huffaker, T. C., and Robbins, P. W. (1983) Yeast mutants deficient in protein glycosylation. Proc. Natl. Acad. Sci. USA 80, 7466-7470
28. Breuer, W., and Bause, E. (1995) Oligosaccharyl transferase is a constitutive component of an oligomeric protein complex from pig liver endoplasmic reticulum. Eur. J. Biochem. 228, 689-696
29. Lee, J., and Coward, J. K. (1993) Oligosaccharyltransferase: synthesis and use of deuterium-labeled peptide substrates as mechanistic probes. Biochemistry 32, 6794-6801
30. Hendrickson, T., and Imperiali, B. (1995) Metal ion dependence of oligosaccharyl transferase: implications for catalysis. Biochemistry 34, 9444-9450
31. Pathak, R., Hendrickson, T. L., and Imperiali, B. (1995) Sulfhydryl modification of the yeast Wbp1p inhibits oligosaccharyl transferase activity. Biochemistry 34, 4179-4185
32. Bosch, M., Trombetta, S., Engstrom, U., and Parodi, A. J. (1988) Characterization of dolichol diphosphate oligosaccharide: protein oligosaccharyltransferase and glycoprotein-processing glucosidases occurring in trypanosomatid protozoa. J. Biol. Chem. 263, 17360-17365
33. Stagljar, I., te Heesen, S., and Aebi, M. (1994) New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: Cloning of the ALG8 locus. Proc. Natl. Acad. Sci. USA 91, 5977-5981
34. Zufferey, R., Knauer, R., Burda, P., Stagljar, I., te Heesen, S., Lehle, L., and Aebi, M. (1995) STT3, a highly conserved protein required for yeast oligosaccharyl transferase activity in vivo. EMBO J., 14, 4949-4960
35. Imperiali, B., and Hendrickson, T. L. (1995) Asparagine linked glycosylation: specificity and function of the oligosaccharyl transferase. Bioorg. Med. Chem. 3, 1565-1578.
36. Clark, R. S., Bannerjee, S., and Coward, J. K. (1990) Yeast oligosaccharyltransferase: glycosylation of peptide substrates and chemical characterization of the glycopeptide product. J. Org. Chem. 55, 6275-6285
37. Imperiali, B., Shannon, K. L., and Rickert, K. W. (1992) Role of peptide conformation in asparagine linked glycosylation. J. Am. Chem. Soc. 114, 7942-7944
38. Imperiali, B., Shannon, K. L., Unno, M., and Rickert, K. W. (1992) A mechanistic proposal for asparagine-linked glycosylation. J. Am. Chem. Soc. 114, 7944-7945
39. Chalifour, R. J., and Spiro, R. G. (1988) Effect of phospholipids on thyroid oligosaccharyltransferase activity and orientation. J. Biol. Chem. 263, 15673-15680
40. Kelleher, D. J., Kreibich, G., and Gilmore, R. (1992) Oligosaccharyltransferase activity is associated with a protein complex composed of ribophorins I and II and a 48 kD protein. Cell 69, 55-65
41. Kumar, V., Heinemann, S., and Ozols, J. (1994) Purification and characterization of avian oligosaccharyltransferase. J. Biol. Chem. 269, 13451-13457
42. Kumar, V., Korza, G., Heinemann, F. S., and Ozols, J. (1995) Human oligosaccharyltransferase: isolation, characterization, and the complete amino acid sequence of the 50-kDa subunit. Arch. Biochem. Biophys. 320, 217-223
43. Kelleher, D. J., and Gilmore, R. (1994) The Saccharomyces cerevisiae oligosaccharyltransferase is a protein complex composed of Wbp1p, Swp1p and four additional polypeptides. J. Biol. Chem. 269, 12908-12917
44. Knauer, R., and Lehle, L. (1994) The N-oligosaccharyltransferase complex from yeast. FEBS Lett. 344, 83-86
45. Kreibich, G., Ulrich, B. L., and Sabatini, D. D. (1978) Proteins of rough microsomal membranes related to ribosome binding I. Identification of ribophorins I and II, membrane proteins characteristic of rough microsomes. J. Cell Biol. 77, 464-487
46. Marcantonio, E. E., Amar-Costesec, A., and Kreibich, G. (1984) Segregation of the polypeptide translocation apparatus to regions of the endoplasmic reticulum containing ribophorins and ribosomes. II. Rat liver microsomal subfractions contain equimolar amounts of ribophorins and ribosomes. J. Cell Biol. 99, 2254-2259
47. Yu, Y., Sabatini, D. D., and Kreibich, G. (1990) Antiribophorin antibodies inhibit the targeting to the ER membrane of ribosomes containing secretory polypeptides. J. Cell Biol. 111, 1335-1342
48. Glabe, C. G., Hanover, J. A., and Lennarz, W. J. (1980) Glycosylation of ovalbumin nascent chains. J. Biol. Chem. 255, 9236-9242
49. Görlich, D., Prehn, S., Hartmann, E., Kalies, K.-U., and Rapoport, T. A. (1992) A mammalian homologue of Sec61p and SecYp is associated with ribosomes and nascent polypeptides during translocation. Cell 71, 489-503
50. Silberstein, S., Kelleher, D. J., and Gilmore, R. (1992) The 48 kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. J. Biol. Chem. 267, 23658-23663
51. Crimaudo, C., Hortsch, M., Gausepohl, H., and Meyer, D. I. (1987) Human ribophorins I and II: the primary structure and membrane topology of two highly conserved endoplasmic reticulum-specific glycoproteins. EMBO J. 6, 75-82
52. Harnik-Ort, V., Prakash, K., Marcantonio, E., Colman, D. R., Rosenfeld, M. G., Adesnik, M., Sabatini, D. D., and Kreibich, G. (1987) Isolation and characterization of cDNA clones for rat ribophorin I: complete coding sequence and in vitro synthesis and insertion of the encoded product into endoplasmic reticulum membranes. J. Cell Biol. 104, 855-863
53. te Heesen, S., Rauhut, R., Aebersold, R., Abelson, J., Aebi, M., and Clark, M. W. (1991) An essential 45 kDa yeast transmembrane protein reacts with anti-nuclear pore antibodies: purification of the protein, immunolocalization and cloning of the gene. Eur. J. Cell Biol. 56, 8-18
54. te Heesen, S., Janetzky, B., Lehle, L., and Aebi, M. (1992) The yeast WBP1 is essential for oligosaccharyltransferase activity in vivo and in vitro. EMBO J. 11, 2071-2075
55. te Heesen, S., Knauer, R., Lehle, L., and Aebi, M. (1993) Yeast Wbp1p and Swp1p form a protein complex essential for oligosaccharyltransferase activity. EMBO J. 12, 279-284
56. Silberstein, S., Collins, P. G., Kelleher, D. J., and Gilmore, R. (1995) The essential OST2 gene encodes the 16 kD subunit of the yeast oligosaccharyltransferase, a highly conserved protein expressed in diverse eukaryotic organisms. J. Cell Biol. 131, 371-383
57. Silberstein, S., Collins, P. G., Kelleher, D. J., Rapiejko, P. J., and Gilmore, R. (1995) The α subunit of the Saccharomyces cerevisiae oligosaccharyltransferase complex is essential for vegetative growth of yeast and is homologous to mammalian ribophorin I. J. Cell Biol. 128, 525-536
58. Karaoglu, D., Kelleher, D. J., and Gilmore, R. (1995) Functional characterization of the Ost3p subunit of the Saccharomyces cerevisiae oligosaccharyltransferase: disruption of the OST3 gene causes biased underglycosylation of acceptor substrates. J. Cell Biol. 130, 567-577
59. Pathak, R., Parker, C. S., and Imperiali, B. (1995) The essential NLT1 gene encodes the 64 kDa glycoprotein subunit of the oligosaccharyl transferase. FEBS Lett. 362, 229-234
60. Roos, J., Sternglanz, R., and Lennarz, W. J. (1994) A screen for yeast mutants with defects in the dolichol-mediated pathway of N-glycosylation. Proc. Natl. Acad. Sci. USA 91, 1485-1489
61. Dean, N. (1995) Yeast glycosylation mutants are sensitive to aminoglycosides. Proc. Natl. Acad. Sci. USA 92, 1287-1291
62. Chi, J. H., Roos, J., and Dean, N. (1996) The OST4 gene of Saccharomyces cerevisiae encodes an unusually small protein required for normal levels of oligosaccharyltransferase activity. J. Biol. Chem. 271, 3132-3140
63. Rose, M. D., Misra, L. M., and Vogel, J. P. (1989) KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene. Cell 57, 1211-1221
64. Klis, F. M. (1994) Review: Cell wall assembly in yeast. Yeast 10, 851-869
65. Ballou, L., Hilzeman, R. A., Lewis, M. S., and Ballou, C. E. (1991) Vanadate-resistant yeast mutants are defective in protein glycosylation. Proc. Natl. Acad. Sci. USA 88, 3209-3212
66. Karaoglu, D., Silberstein, S., Kelleher, D. J., and Gilmore, R. The Saccharomyces cerevisiae oligosaccharyltransferase: a large hetero-oligomeric complex in the endoplasmic reticulum. Cold Spring Harbor Symp. Quant. Biol. In press
67. Schutzbach, J. S., Zimmerman, J. W., and Forsee, W. T. (1993) The purification and characterization of recombinant yeast dolichyl-phosphate-mannose synthase: Site directed mutagenesis of the putative dolichol recognition sequence. J. Biol. Chem. 268, 24190-24196
68. Stagljar, I., Aebi, M., and te Heesen, S. (1995) PCR-mediated cloning and sequencing of the DmOST50 gene, a WBP1/AvOST50/OST48 homologue, from Drosophila melanogaster. Gene 158, 209-212
69. Nakashima, T., Sekiguchi, T., Kuraoka, A., Fukushima, K., Shibata, Y., Komiyama, S., and Nishimoto, T. (1993) Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell. Biol. 13, 6367-6374