A ligand-dependent conformational change of the Na+/galactose cotransporter of Vibrio parahaemolyticus, monitored by tryptophan fluorescence

Journal of Membrane Biology

Volumn 185, Issue 3, 2002, Pages 249-255

  Veenstra, M ^a   Turk, E ^a   Wright, E M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Conformational change; Na+ galactose transporter; Proteoliposomes; Quench reagents; Tryptophan fluorescence

Indexed keywords

GALACTOSE; LIGAND; SODIUM ION; TRYPTOPHAN;

ARTICLE; ESCHERICHIA COLI; FLUORESCENCE; GENE EXPRESSION; ION TRANSPORT; MEMBRANE TRANSPORT; NONHUMAN; PROTEOLIPOSOME; SITE DIRECTED MUTAGENESIS; VIBRIO PARAHAEMOLYTICUS;

CALCIUM-BINDING PROTEINS; FLUORESCENCE; GALACTOSE; ION TRANSPORT; MONOSACCHARIDE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; PERIPLASMIC BINDING PROTEINS; PROTEIN CONFORMATION; PROTEOLIPIDS; SODIUM; SUBSTRATE SPECIFICITY; TRYPTOPHAN; VIBRIO PARAHAEMOLYTICUS;

ESCHERICHIA COLI; VIBRIO; VIBRIO PARAHAEMOLYTICUS;

EID: 0036469113     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-001-0127-9     Document Type: Article

References (21)

1. Exposure of tryptophanyl residues in proteins, quantitative determination by fluorescence quenching studies
2. 2
3. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
4. Increased sensitivity for Coomassie staining of sodium dodecyl sulfate-polyacrylamide gels using PhastSystem Development Unit
5. +/glucose cotransporter
6. + and glucose transport
7. Intrinsic fluorescence of the P-glycoprotein multidrug transporter: Sensitivity of tryptophan residues to binding of drugs and nucleotides
8. Melibiose permease of Escherichia coli: Structural organization of cosubstrate binding sites as deduced from tryptophan fluorescence analyses
9. Melibiose Permease of Escherichia coli: Substrate-induced conformational changes monitored by tryptophan fluorescence spectroscopy
10. +/glucose transporter
11. +/glucose cotransporter (SGLT1) expressed in E. coli
12. +/glucose cotransporters is determined by the C-terminal half of the protein
13. Ligand-Dependent quenching of tryptophan fluorescence in human erythrocyte hexose transport protein
14. +-induced conformational change of the intestinal brush border Na/glucose symporter using fluorescent probes
15. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
16. Molecular characterization of Vibrio parahaemolyticus vSGLT: A model for sodium-coupled sugar cotransporters
17. Membrane Topological Motifs in the SGLT Cotransporter Family
18. +/glucose transport
19. +/glucose Cotransporter
20. +/glucose Cotransporter: A bacterial member of the SGLT Family