Increased stability and specificity through combined hybridization of peptide nucleic acid (PNA) and locked nucleic acid (LNA) to supercoiled plasmids for PNA-anchored "Bioplex" formation

Biomolecular Engineering

Volumn 22, Issue 5-6, 2005, Pages 185-192

  Lundin, Karin E ^a   Hasan, Maroof ^a   Moreno, Pedro M ^a   Törnquist, Elisabeth ^a   Oprea, Iulian ^a   Svahn, Mathias G ^a   Simonson, E Oscar ^a   Smith, C I Edvard ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Cooperative binding; Locked nucleic acid; Peptide nucleic acid; PNA DNA hybridization; Supercoiled DNA

Indexed keywords

GENES; OLIGOMERS; POLYPEPTIDES; PURIFICATION; STABILIZATION;

COOPERATIVE BINDING; LOCKED NUCLEIC ACID; PEPTIDE NUCLEIC ACID; PNA-DNA HYBRIDIZATION; SUPERCOILED DNA;

NUCLEIC ACIDS;

LOCKED NUCLEIC ACID; OLIGOMER; PEPTIDE NUCLEIC ACID; PLASMID DNA; SODIUM CHLORIDE;

ARTICLE; BINDING KINETICS; COMPLEX FORMATION; DNA BINDING; DNA HYBRIDIZATION; DNA STRAND; DNA SUPERCOILING; INCUBATION TIME; MOLECULAR INTERACTION; MOLECULAR STABILITY; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL;

ANIMALS; DNA, SUPERHELICAL; GENE THERAPY; HUMANS; KINETICS; NUCLEIC ACID HYBRIDIZATION; PEPTIDE NUCLEIC ACIDS; PLASMIDS;

EID: 27944467877     PISSN: 13890344     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bioeng.2005.07.003     Document Type: Article

References (32)

1. B. Nasto Questions about systemic adenovirus delivery Mol Ther 5 2002 652 653
2. M.P. McCormack, and T.H. Rabbitts Activation of the T-cell oncogene LMO2 after gene therapy for X-linked severe combined immunodeficiency N Engl J Med 350 2004 913 922
3. P. Blomberg, and C.I. Smith Gene therapy of monogenic and cardiovascular disorders Expert Opin Biol Ther 3 2003 941 949
4. P. Collas, H. Husebye, and P. Alestrom The nuclear localization sequence of the SV40 T antigen promotes transgene uptake and expression in zebrafish embryo nuclei Transgenic Res 5 1996 451 458
5. L.J. Branden, A.J. Mohamed, and C.I. Smith A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA Nat Biotechnol 17 1999 784 787
6. M.A. Zanta, P. Belguise-Valladier, and J.P. Behr Gene delivery: a single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus Proc Natl Acad Sci USA 96 1999 91 96
7. C.K. Chan, and D.A. Jans Using nuclear targeting signals to enhance non-viral gene transfer Immunol Cell Biol 80 2002 119 130
8. F.M. Munkonge, D.A. Dean, E. Hillery, U. Griesenbach, and E.W. Alton Emerging significance of plasmid DNA nuclear import in gene therapy Adv Drug Deliv Rev 55 2003 749 760
9. C. Uherek, and W. Wels DNA-carrier proteins for targeted gene delivery Adv Drug Deliv Rev 44 2000 153 166
10. P.E. Nielsen, M. Egholm, R.H. Berg, and O. Buchardt Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide Science 254 1991 1497 1500
11. P.E. Nielsen PNA technology Methods Mol Biol 208 2002 3 26
12. R. Kumar, S.K. Singh, A.A. Koshkin, V.K. Rajwanshi, M. Meldgaard, and J. Wengel The first analogues of LNA (locked nucleic acids): phosphorothioate-LNA and 2′-thio-LNA Bioorg Med Chem Lett 8 1998 2219 2222
13. S. Obika, D. Nanbu, Y. Had, J.-I. Andoh, K.-I. Morio, and T. Doi Stability and structural features of the duplexes containing nucleoside analogues with a fixed N-type conformation, 2′-O,4′-C- methyleneribonucleosides Tetrahed Lett 39 1998 5401 5404
14. M. Petersen, and J. Wengel LNA: a versatile tool for therapeutics and genomics Trends Biotechnol 21 2003 74 81
15. A.G. Rebuff at, A.R. Nawrocki, P.E. Nielsen, A.G. Bernasconi, E. Bernal-Mendez, and B.M. Frey Gene delivery by a steroid-peptide nucleic acid conjugate FASEB J 16 2002 1426 1428
16. K.M. Hertoghs, J.H. Ellis, and I.R. Catchpole Use of locked nucleic acid oligonucleotides to add functionality to plasmid DNA Nucleic Acids Res 31 2003 5817 5830
17. L.J. Branden, and C.I. Smith Bioplex technology: novel synthetic gene delivery system based on peptides anchored to nucleic acids Methods Enzymol 346 2002 106 124
18. M. Egholm, L. Christensen, K.L. Dueholm, O. Buchardt, J. Coull, and P.E. Nielsen Efficient pH-independent sequence-specific DNA binding by pseudoisocytosine-containing bis-PNA Nucleic Acids Res 23 1995 217 222
19. H. Kuhn, V.V. Demidov, P.E. Nielsen, and M.D. Frank-Kamenetskii An experimental study of mechanism and specificity of peptide nucleic acid (PNA) binding to duplex DNA J Mol Biol 286 1999 1337 1345
20. J. Lohse, O. Dahl, and P.E. Nielsen Double duplex invasion by peptide nucleic acid: a general principle for sequence-specific targeting of double-stranded DNA Proc Natl Acad Sci USA 96 1999 11804 11808
21. A.A. Koshkin, S.K. Singh, P. Nielsen, V.K. Rajwanshi, R. Kumar, and M. Meldgaard LNA (locked nucleic acids): synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition Tetrahedron 54 1998 3607 3630
22. J. Wengel Synthesis of 3′-C- and 4′-C-branched oligodeoxynucleotides and the development of locked nucleic acid (LNA) Acc Chem Res 32 1999 301 310
23. C. Wahlestedt, P. Salmi, V. Good, J. Kela, T. Johnsson, and T. Hökfelt Potent and nontoxic antisense oligonucleotides containing locked nucleic acids Proc Natl Acad Sci USA 97 2000 5633 5638
24. J. Jepsen, and J. Wengel LNA-antisense rivals siRNA for gene silencing Curr Opin Drug Disc Dev 7 2004 188 194
25. R. Crinelli, M. Bianchi, L. Gentilini, L. Palma, M.D. Sorensen, and T. Bryld Transcription factor decoy oligonucleotides modified with locked nucleic acids: an in vitro study to reconcile biostability with binding affinity Nucleic Acids Res 32 2004 1874 1885
26. V.V. Demidov, V.N. Potaman, M.D. Frank-Kamenetskii, M. Egholm, O. Buchard, and S.H. Sonnichsen Stability of peptide nucleic acids in human serum and cellular extracts Biochem Pharmacol 48 1994 1310 1313
27. K.E. Lundin, R. Ge, M.G. Svahn, E. Tornquist, M. Leijon, and L.J. Branden Cooperative strand invasion of supercoiled plasmid DNA by mixed linear PNA and PNA-peptide chimeras Biomol Eng 21 2004 51 59
28. M.G. Svahn, K.E. Lundin, R. Ge, E. Tornquist, E.O. Simonson, and S. Oscarsson Adding functional entities to plasmids J Gene Med 6 Suppl 1 2004 36 S44
29. H. Kuhn, V.V. Demidov, M.D. Frank-Kamenetskii, and P.E. Nielsen Kinetic sequence discrimination of cationic bis-PNAs upon targeting of double-stranded DNA Nucleic Acids Res 26 1998 582 587
30. L.J. Branden, B. Christensson, and C.I. Smith In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides Gene Ther 8 2001 84 87
31. T. Bentin, and P.E. Nielsen PNA-mediated immobilization of supercoiled DNA Methods Mol Biol 208 2002 111 118
32. K. Kaihatsu, D.A. Braasch, A. Cansizoglu, and D.R. Corey Enhanced strand invasion by peptide nucleic acid-peptide conjugates Biochemistry 41 2002 11118 11125