In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides

Gene Therapy

Volumn 8, Issue 1, 2001, Pages 84-87

  Branden L J ^a,b   Christensson, B ^a   Smith, C I Edvard ^a,b  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Gene therapy; In vivo; NLS; PNA; Transfection

Indexed keywords

FLUORESCENT DYE; HYBRID PROTEIN; OLIGONUCLEOTIDE; PEPTIDE NUCLEIC ACID;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; GENE TARGETING; GENE TRANSLOCATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HYBRIDIZATION; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

ANIMALIA;

EID: 0035135576     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301345     Document Type: Article

References (10)

1. Peptide nucleic acids: On the road to new gene therapeutic drugs
2. A long synthetic peptide containing a nuclear localization signal and its flanking sequences of SV40 T-antigen directs the transport of IgM into the nucleus efficiently
3. A peptide nucleic acid-nuclear localization signal fusion that mediates nuclear transport of DNA
4. Cyanine dye labeling reagents: Sulfoindocyanine succinimidyl esters
5. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
6. Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery
7. Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors
8. Cloning of two novel human importin-α subunits and analysis of the expression pattern of the importin-α protein family
9. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA