Gene expression in mammalian cells after exposure to 95 MeV/amu argon ions

Advances in Space Research

Volumn 36, Issue 9, 2005, Pages 1680-1688

  Arenz, Andrea ^a   Hellweg, Christine E ^a   Meier, Matthias M ^a   Baumstark Khan, Christa ^a  

^a GERMAN AEROSPACE CENTER DLR   (Germany)

Author keywords

Gene activation; Human radiation responsive genes; Quantitative RT PCR; Space

Indexed keywords

CELL CULTURE; CELLS; GENES; GENETIC ENGINEERING; X RAYS;

BIOLOGICAL RISKS; GENE ACTIVATION; GENE EXPRESSION; GENE MUTATION; HUMAN RADIATION-RESPONSIVE GENES; MAMMALIAN CELLS; QUANTITATIVE RT-PCR;

SPACE RESEARCH;

EID: 27844464545     PISSN: 02731177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.asr.2005.02.062     Document Type: Article

References (28)

1. G.S. Akerman, B.A. Rosenzweig, and O.E. Domon Alterations in gene expression profiles and the DNA-damage response in ionizing radiation exposed TK6 cell Environ. Mol. Mutagen. 45 2005 188 205
2. S.A. Amundson, M. Bittner, and P. Meltzer Induction of gene expression as a monitor of exposure to ionizing radiation Radiat. Res. 156 2001 657 661
3. F. Bunz, and A. Dutriaux Requirement for p53 and p21 to sustain G2 arrest after DNA damage Science 282 1998 1497 1501
4. J. Chen, D.P. Silver, and D. Walpita Stable interaction between the products of the BRCA1 and BRCA2 tumor suppressor genes in mitotic and meiotic cells Molec. Cell. 2 1998 317 328
5. E. De Smaele, F. Zazzeroni, and S. Papa Induction of gadd45 beta by NF-kappa-B downregulates pro-apoptotic JNK signaling Nature 414 2001 308 313
6. W.S. El-Diery, T. Tokino, and V.E. Velculisci WAF1, a potential mediator of 53 tumour suppression Cell 75 1993 817 825
7. P. Fei, and W.S. El-Diery p53 and radiation responses Oncogene 22 2003 5774 5783
8. G. Horneck, R. Facius, and M. Reichert HUMEX, a study on the survivability and adaptation of humans to long-duration exploratory missions, part I: lunar missions Adv. Space Res. 31 11 2003 2389 2401
9. Horneck, G., Facius, R., Reichert, M., et al. HUMEX, a study on the survivability and adaptation of humans to long-duration exploratory missions ESA SP-1264, 2003b.
10. G. Iliakis, Y. Wang, and J. Guan DNA damage checkpoint control in cells exposed to ionizing radiation Oncogene 22 2003 5834 5847
11. S.P. Jackson Sensing and repairing DNA double-strand breaks Carcinogenesis 23 2002 687 696
12. O. Jakel Radiation hazard during a manned mission to Mars Z. Med. Phys. 14 4 2004 267 272
13. S.M. Morris A role for p53 in the frequency and mechanism of mutation Mutat. Res. 511 2002 45 62
14. M.W. Pfaffl, G.W. Horgan, and L. Dempfle Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR Nucleic Acids Res. 30 2002 e36
15. M.W. Pfaffl, A. Tichopad, and C. Prgomet Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations Biotechnol. Lett. 26 2004 509 515
16. M.W. Pfaffl A new mathematical model for relative quantification in real-time RT-PCR Nucleic Acids Res. 29 2001 e45
17. R. Rasmussen Quantification on the LightCycler S. Meuer C. Wittwer K. Nakagawara Rapid cycle real time PCR: methods and applications 2001 Springer Heidelberg 21 34
18. J.L. Redpath Radiation-induced neoplastic transformation in vitro: evidence for a protective effect at low doses of low LET radiation Cancer Metast. Rev. 23 2004 333 339
19. V. Serazin-Leroy, D. Denis-Henriot, and M. Morot Semi-quantitative RT-PCR for comparison of mRNAs in cells with different amounts of housekeeping gene transcripts Mol. Cell. Probes 12 1998 283 291
20. S.K. Sharan, M. Morimatsu, and U. Albrecht Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2 Nature 386 1997 804 810
21. T. Suzuki, P.J. Higgins, and D.R. Crawford Control selection for RNA quantitation Biotechniques 29 2000 332 337
22. H. Tanaka, H. Arakawa, and T. Yamaguchi A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage Nature 404 2000 42 49
23. O. Thellin, W. Zorzi, and B. Lakaye Housekeeping genes as internal standards: use and limits J. Biotechnol. 75 1999 291 295
24. M. Vairapandi, A.G. Balliet, and B. Hoffman GADD45b and GADD45g are cdc2/cyclinB1 kinase inhibitors with a role in S and G2/M cell cycle checkpoints induced by genotoxic stress J. Cell. Physiol. 192 2002 327 338
25. Vandesompele, J., De Preter, K., Pattyn, F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3(7): research 0034.1-research 0034.11, 2002.
26. G.E. Woloschak, and C.M. Chang-Liu Differential modulation of specific gene expression following high- and low-LET radiations Radiat. Res. 124 1990 183 187
27. K. Wu, S.W. Jiang, and F.J. Couch p53 mediates repression of the BRCA2 promoter and down-regulation of BRCA2 mRNA and protein levels in response to DNA damage J. Biol. Chem. 278 2003 15652 15660
28. H.W. Wulf, H.G. Kraft-Weyrather, and E.A. Miltenburger Heavy ions effects on mammalian cells inactivation measurements with different cell lines Radiat. Res. Suppl. 8 1985 S122 S134