Measuring Ras-family GTP levels in vivo - Running hot and cold

Methods

Volumn 37, Issue 2, 2005, Pages 190-196

  Castro, Ariel F ^a   Rebhun, John F ^a   Quilliam, Lawrence A ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

GTP; GTPase; Guanine nucleotide exchange; M Ras; R Ras; Rap1; Ras; Ras binding domain; Rheb

Indexed keywords

ANTIBODY; EPITOPE; GLUTATHIONE TRANSFERASE; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; GUANOSINE TRIPHOSPHATE; LIGAND; NUCLEOTIDE; RAF PROTEIN; RAP PROTEIN; RAS PROTEIN;

ARTICLE; ENZYME ACTIVITY; IMMUNOBLOTTING; IMMUNOPRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN FUNCTION; THIN LAYER CHROMATOGRAPHY;

ANIMALS; BINDING SITES; CELLS, CULTURED; COLD; GLUTATHIONE TRANSFERASE; GUANOSINE TRIPHOSPHATE; HEAT; HUMANS; IMMUNOPRECIPITATION; PROTEIN STRUCTURE, TERTIARY; RAS PROTEINS; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING; TRANSFECTION;

EID: 27744583596     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2005.05.015     Document Type: Article

References (36)

1. L.A. Quilliam, Ras Family, in: M.D. Lane, W.J. Lennarz (Eds.), Encyclopedia of Biological Chemistry, vol. 3, Elsevier, Oxford, 2004, pp. 640-644.
2. L.A. Quilliam, J.F. Rebhun, and A.F. Castro A growing number of guanine nucleotide exchange factors is responsible for activation of ras family GTPases Prog. Nucleic Acid Res. Mol. Biol. 71 2002 391 444
3. I.R. Vetter, and A. Wittinghofer The guanine nucleotide-binding switch in three dimensions Science 294 2001 1299 1304
4. P.J. Lockyer, S. Kupzig, and P.J. Cullen CAPRI regulates Ca(2+)-dependent inactivation of the Ras-MAPK pathway Curr. Biol. 11 2001 981 986
5. A.F. Castro, J.F. Rebhun, G.J. Clark, and L.A. Quilliam Rheb binds TSC2 and promotes S6 kinase activation in a rapamycin- and farnesylation-dependent manner J. Biol. Chem. 278 2003 32493 32496
6. J.D. Jordan, K.D. Carey, P.J. Stork, and R. Iyengar Modulation of rap activity by direct interaction of Galpha(o) with Rap1 GTPase-activating protein J. Biol. Chem. 274 1999 21507 21510
7. S.L. Campbell, R. Khosravi-Far, K.L. Rossman, G.J. Clark, and C.J. Der Increasing complexity of Ras signaling Oncogene 17 1998 1395 1413
8. J. de Rooij, and J.L.. Bos Minimal Ras-binding domain of Raf1 can be used as an activation-specific probe for Ras Oncogene 14 1997 623 625
9. S.J. Taylor, and D. Shalloway Cell cycle-dependent activation of Ras Curr. Biol. 6 1996 1621 1627
10. 2+-mediated activation of Rap1 in human platelets Embo J. 16 1997 252 259
11. J.F. Rebhun, A.F. Castro, and L.A. Quilliam Identification of guanine nucleotide exchange factors (GEFs) for the rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction J. Biol. Chem. 275 2000 34901 34908
12. R.M. Wolthuis, B. Franke, M. van Triest, B. Bauer, R.H. Cool, J.H. Camonis, J.W. Akkerman, and J.L. Bos Activation of the small GTPase Ral in platelets Mol. Cell. Biol. 18 1998 2486 2491
13. J.F. Rebhun, H. Chen, and L.A. Quilliam Identification and characterization of a new family of guanine nucleotide exchange factors for the Ras-related GTPase Ral J. Biol. Chem. 275 2000 13406 13410
14. L.A. Quilliam, K.R. Graham, A.F. Castro, C.B. Martin, C.J. Der, and C. Bi M-Ras/R-Ras3, a novel transforming Ras protein regulated by Sos1 and p120 GAP, interacts with the putative Ras effector AF6 J. Biol. Chem. 1999 23850 23857
15. L.A. Quilliam, J.F. Rebhun, H. Zong, and A.F. Castro Analysis of M Ras/R-Ras3 signaling and biology Methods Enzymol. 333 2001 187 202
16. A. Ehrhardt, M.D. David, G.R. Ehrhardt, and J.W. Schrader Distinct mechanisms determine the patterns of differential activation of H-Ras, N-Ras, K-Ras 4B, and M-Ras by receptors for growth factors or antigen Mol. Cell. Biol. 24 2004 6311 6323
17. X.D. Ren, and M.A. Schwartz Determination of GTP loading on Rho Methods Enzymol. 325 2000 264 272
18. J.A. Glaven, I. Whitehead, S. Bagrodia, R. Kay, and R.A. Cerione The Dbl-related protein, Lfc, localizes to microtubules and mediates the activation of Rac signaling pathways in cells J. Biol. Chem. 274 1999 2279 2285
19. V. Benard, and G.M. Bokoch Assay of Cdc42, Rac, and Rho GTPase activation by affinity methods Methods Enzymol. 345 2002 349 359
20. J. Downward, J.D. Graves, P.H. Warne, S. Rayter, and D.A. Cantrell Stimulation of p21ras upon T-cell activation Nature 346 1990 719 723
21. J.B. Gibbs, M.S. Marshall, E.M. Scolnick, R.A. Dixon, and U.S. Vogel Modulation of guanine nucleotides bound to Ras in NIH3T3 cells by oncogenes, growth factors, and the GTPase activating protein (GAP) J. Biol. Chem. 265 1990 20437 20442
22. Y.W. Fridell, Y. Jin, L.A. Quilliam, A. Burchert, P. McCloskey, G. Spizz, B. Varnum, C. Der, and E.T. Liu Differential activation of the Ras/extracellular-signal-regulated protein kinase pathway is responsible for the biological consequences induced by the Axl receptor tyrosine kinase Mol. Cell. Biol. 16 1996 135 145
23. J.S. Scheele, J.M. Rhee, and G.R. Boss Determination of absolute amounts of GDP and GTP bound to Ras in mammalian cells: comparison of parental and Ras-overproducing NIH 3T3 fibroblasts Proc. Natl. Acad. Sci. USA 92 1995 1097 1100
24. F.C. von Lintig, A.D. Dreilinger, N.M. Varki, A.M. Wallace, D.E. Casteel, and G.R. Boss Ras activation in human breast cancer Breast Cancer Res. Treat. 62 2000 51 62
25. N. Lau, E.J. Uhlmann, F.C. Von Lintig, A. Nagy, G.R. Boss, D.H. Gutmann, and A. Guha Rap1 activity is elevated in malignant astrocytomas independent of tuberous sclerosis complex-2 gene expression Int. J. Oncol. 22 2003 195 200
26. E. Im, F.C. von Lintig, J. Chen, S. Zhuang, W. Qui, S. Chowdhury, P.F. Worley, G.R. Boss, and R.B. Pilz Rheb is in a high activation state and inhibits B-Raf kinase in mammalian cells Oncogene 21 2002 6356 6365
27. N. Mochizuki, S. Yamashita, K. Kurokawa, Y. Ohba, T. Nagai, A. Miyawaki, and M. Matsuda Spatio-temporal images of growth-factor-induced activation of Ras and Rap1 Nature 411 2001 1065 1068
28. O. Pertz, and K.M. Hahn Designing biosensors for Rho family proteins-deciphering the dynamics of Rho family GTPase activation in living cells J. Cell Sci. 117 2004 1313 1318
29. T.G. Bivona, H.H. Wiener, I.M. Ahearn, J. Silletti, V.K. Chiu, and M.R. Philips Rap1 up-regulation and activation on plasma membrane regulates T cell adhesion J. Cell Biol. 164 2004 461 470
30. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular Cloning: A Laboratory Manual 1989 Cold Spring Harbor Laboratory Press Cold Spring harbor, NY
31. T.R. Brtva, J.K. Drugan, S. Ghosh, R.S. Terrell, S. Campbell-Burk, RM. Bell, and CJ. Der Two distinct Raf domains mediate interaction with Ras J. Biol. Chem. 270 1995 9809 9812
32. F.L. Graham, and A.J. van der Eb A new technique for the assay of infectivity of human adenovirus 5 DNA Virology 52 1973 456 467
33. P.G. Chesa, W.J. Rettig, M.R. Melamed, L.J. Old, and H.L. Niman Expression of p21ras in normal and malignant human tissues: lack of association with proliferation and malignancy Proc. Natl. Acad. Sci. USA 84 1987 3234 3238
34. M.E. Furth, L.J. Davis, B. Fleurdelys, and E.M. Scolnick Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family J. Virol. 43 1982 294 304
35. A. Garami, F.J. Zwartkruis, T. Nobukuni, M. Joaquin, M. Roccio, H. Stocker, S.C. Kozma, E. Hafen, J.L. Bos, and G. Thomas Insulin activation of Rheb, a mediator of mTOR/S6K/4E-BP signaling, is inhibited by TSC1 and 2 Mol. Cell 11 2003 1457 1466
36. M. Izquierdo, J. Downward, H. Otani, W.J. Leonard, and D.A. Cantrell Interleukin (IL)-2 activation of p21ras in murine myeloid cells transfected with human IL-2 receptor beta chain Eur. J. Immunol. 22 1992 817 821