Designing biosensors for Rho family proteins - Deciphering the dynamics of Rho family GTPase activation in living cells

Journal of Cell Science

Volumn 117, Issue 8, 2004, Pages 1313-1318

  Pertz, Olivier ^a   Hahn, Klaus M ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Cell motility; Dye; Fluorescence resonance energy transfer; Microscopy; Ratio imaging; Rho family GTPase

Indexed keywords

FLUORESCENT DYE; PROTEIN CDC42; RAC PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; CELL MOTILITY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME REGULATION; ENZYMIC BIOSENSOR; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; SENSITIVITY AND SPECIFICITY; SIGNAL TRANSDUCTION;

ANIMALS; BIOSENSING TECHNIQUES; CDC42 GTP-BINDING PROTEIN; CELL MOVEMENT; ENZYME ACTIVATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HUMANS; KINETICS; RAC GTP-BINDING PROTEINS; RHO GTP-BINDING PROTEINS; RHOA GTP-BINDING PROTEIN; SENSITIVITY AND SPECIFICITY;

EID: 2342436482     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: 10.1242/jcs.01117     Document Type: Article

References (33)

1. Abe, T., Kato, M., Miki, H., Takenawa, T. and Endo, T. (2003). Small GTPase Tc10 and its homologue RhoT induce N-WASP-mediated long process formation and neurite outgrowth. J. Cell Sci. 116, 155-168.
2. Cannon, J. L., Labno, C. M., Bosco, G., Seth, A., McGavin, M. H., Siminovitch, K. A., Rosen, M. K. and Burkhardt, J. K. (2001). Wasp recruitment to the T cell: APC contact site occurs independently of Cdc42 activation. Immunity 15, 249-259.
3. Chamberlain, C. E., Kraynov, V. S. and Hahn, K. M. (2000). Imaging spatiotemporal dynamics of Rac activation in vivo with FLAIR. Methods Enzynol. 325, 389-400.
4. Dawson, P. E. and Kent, S. B. (2000). Synthesis of native proteins by chemical ligation. Annu. Rev. Biochem. 69, 923-960.
5. Del Pozo, M. A., Kiosses, W. B., Alderson, N. B., Meller, N., Hahn, K. M. and Schwartz, M. A. (2002). lntegrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI. Nat. Cell Biol. 4, 232-239.
6. Etienne-Manneville, S. and Hall, A. (2002). Rho GTPases in cell biology. Nature 420, 629-635.
7. Gardiner, E. M., Pestonjamasp, K. N., Bohl, B. P., Chamberlain, C., Hahn, K. M. and Bokoch, G. M. (2002). Spatial and temporal analysis of Rac activation during live neutrophil chemotaxis. Curr. Biol. 12, 2029-2034.
8. Hoffman, G. R. and Cerione, R. A. (2000). Flipping the switch: the structural basis for signaling through the CRIB motif. Cell 102, 403-406.
9. Hoffman, G. R., Nassar, N. and Cerione, R. A. (2000). Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDI. Cell 100, 345-356.
10. Itoh, R. E., Kurokawa, K., Ohba, Y., Yoshizaki, H., Mochizuki, N. and Matsuda, M. (2002). Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane of living cells. Mol. Cell. Biol. 22, 6582-6591.
11. Kim, S. H., Li, Z. and Sacks, D. B. (2000). E-eadherin-mediated cell-cell attachment activates Cdc42. J. Biol. Chem. 275, 36999-37005.
12. Kranenburg, O., Poland, M., Gebbink, M., Oomen, L. and Moolenaar, W. H. (1997). Dissociation of LPA-induced cytoskeletal contraction from stress fiber formation by differential localization of RhoA. J. Cell Sci. 110, 2417-2427.
13. Kraynov, V. S., Chamberlain, C., Bokoch, G. M., Schwartz, M. A., Slabaugh, S. and Hahn, K. M. (2000). Localized Rac activation dynamics visualized in living cells. Science 290, 333-337.
14. Merlot, S. and Firtel, R. A. (2003). Leading the way: directional sensing through phosphatidylinositol 3-kinase and other signaling pathways. J. Cell Sci. 116, 3471-3478.
15. Michaelson, D., Silletti, J., Murphy, G., D'Eustachio, P., Rush, M. and Philips, M. R. (2001). Differential localization of Rho GTPases in live cells: regulation by hypervariable regions and RhoGDI binding. J. Cell Biol. 152, 111-126.
16. Miyawaki, A. (2003). Visualization of the spatial and temporal dynamics of intracellular signaling. Dev. Cell 4, 295-305.
17. Mochizuki, N., Yamashita, S., Kurokawa, K., Ohba, Y., Nagai, T., Miyawaki, A. and Matsuda, M. (2001). Spatio-temporal images of growth-factor-induced activation of Ras and Rap1. Nature 411, 1065-1068.
18. Mott, H. R., Owen, D., Nietlispach, D., Lowe, P. N., Manser, E., Lim, L. and Laue, E. D. (1999). Structure of the small G protein Cdc42 bound to the GTPase-binding domain of ACK. Nature 399, 384-388.
19. Nassar, N., Hoffman, G. R., Manor, D., Clardy, J. C. and Cerione, R. A. (1998). Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP. Nat. Struct. Biol. 5, 1047-1052.
20. Nimnual, A. S., Taylor, L. J. and Bar-Sagi, D. (2003). Redox-dependent downregulation of Rho by Rac. Nat. Cell Biol. 5, 236-241.
21. Nobes, C. D. and Hall, A. (1995). Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia. and filopodia. Cell 81, 53-62.
22. Randazzo, P. A., Nie, Z., Miura, K. and Hsu, V. W. (2000). Molecular aspects of the cellular activities of ADP-ribosylation factors. Sci STKE 2000, RE1.
23. Reid, T., Furuyashiki, T., Ishizaki, T., Watanabe, G., Watanabe, N., Fujisawa, K., Morii, N., Madaule, P. and Narumiya, S. (1996). Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the rho-binding domain. J. Biol. Chem. 271, 13556-13560.
24. Sasaki, T., Kato, M. and Takai, Y. (1993). Consequences of weak interaction of rho GDI with the GTP-bound forms of rho p21 and rac p21. J. Biol. Chem. 268, 23959-23963.
25. Seth, A., Otomo, T., Yin, H. L. and Rosen, M. K. (2003). Rational design of genetically encoded fluorescence resonance energy transfer-based sensors of cellular Cdc42 signaling. Biochemistry 42, 3997-4008.
26. Srinivasan, S., Wang, F., Glavas, S., Ott, A., Hofmann, F., Aktories, K., Kalman, D. and Bourne, H. R. (2003). Rac and Cdc42 play distinct roles in regulating PI(3,4,5)P3 and polarity during neutrophil chemotaxis. J. Cell Biol. 160, 375-385.
27. Thompson, G., Owen, D., Chalk, P. A. and Lowe, P. N. (1998). Delineation of the Cdc42/Rac-binding domain of p21-activated kinase. Biochemistry 37, 7885-7891.
28. Toutchkine, A., Kraynov, V. S. and Hahn, K. M. (2003). Solvent-sensitive dyes to report protein conformational changes in living cells. J. Am. Chem. Soc. 125, 4132-4145.
29. Tsuji, T., Ishizaki, T., Okamoto, M., Higashida, C., Kimura, K., Furuyashiki, T., Arakawa, Y., Birge, R. B., Nakamoto, T., Hirai, H. et al. (2002). ROCK and mDia1 antagonize in Rho-dependent Rac activation in Swiss 3T3 fibroblasts. J. Cell Biol. 157, 819-830.
30. Vignal, E., de Toledo, M., Comunale, F., Ladopoulou, A., Gauthier-Rouviere, C., Blangy, A. and Fort, P. (2000). Characterization of TCL, a new GTPase of the rho family related to TC10 andCcdc42. J. Biol. Chem. 275, 36457-36464.
31. Wang, L., Brock, A,, Herberich, B. and Schultz, P. G. (2001). Expanding the genetic code of Escherichia coli. Science 292, 498-500.
32. Worthylake, R. A., Lemoine, S., Watson, J. M. and Burridge, K. (2001). RhoA is required for monocyte tail retraction during transendothelial migration. J. Cell Biol. 154, 147-160.
33. Yoshizaki, H., Ohba, Y., Kurokawa, K., Itoh, R. E., Nakamura, T., Mochizuki, N., Nagashima, K. and Matsuda, M. (2003). Activity of Rho-family GTPases during cell division as visualized with FRET-based probes. J. Cell Biol. 162, 223-232.