Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR

Journal of Medical Virology

Volumn 77, Issue 4, 2005, Pages 602-608

  Keightley, Maria Cristina ^a,b   Sillekens, Peter ^c   Schippers, Wim ^c   Rinaldo, Charles ^a,b,e   St George, Kirsten ^a,b,d  

^a UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

^b UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^c Department of BioMérieux ^*  (Netherlands)

^d WADSWORTH CENTER   (United States)

^e PRESBYTERIAN UNIVERSITY HOSPITAL   (United States)

Author keywords

Coronavirus; NASBA; RT PCR; SARS

Indexed keywords

VIRUS RNA;

ARTICLE; CORONAVIRUS; INTERMETHOD COMPARISON; NONHUMAN; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SARS CORONAVIRUS; VIRUS CULTURE; VIRUS DETECTION; VIRUS INFECTION;

HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SARS VIRUS; SEVERE ACUTE RESPIRATORY SYNDROME;

CORONAVIRUS; RNA VIRUSES; SARS ASSOCIATED CORONAVIRUS; SARS CORONAVIRUS; SARS COV;

EID: 27744568291     PISSN: 01466615     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmv.20498     Document Type: Article

References (25)

1. Bressler AM, Nolte FS. 2004. Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus. J Clin Microbiol 42:987-991.
2. Brown EG, Tetro JA. 2003. Comparative analysis of the SARS coronavirus genome: A good start to a long journey. Lancet 361: 1756-1757.
3. Chan PK. 2003. Human metapneumovirus detection in patients with severe acute respiratory syndrome. Emerg Infect Dis 9:1058-1063.
4. Chen X, Zhou B, Li M, Liang X, Wang H, Yang G, Wang H, Le X. 2004. Serology of severe acute respiratory syndrome: Implications for surveillance and outcome. J Infect Dis 189:1158-1163.
5. Drosten C, Gunther S, Preiser W, van der WS, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, Berger A, Burguiere AM, Cinatl J, Eickmann M, Escriou N, Grywna K, Kramme S, Manuguerra JC, Muller S, Rickerts V, Sturmer M, Vieth S, Klenk HD, Osterhaus AD, Schmitz H, Doerr HW. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med 348:1967-1976.
6. Drosten C, Chiu L-L, Panning M, Leong HN, Preiser W, Tam JS, Gunther S, Kramme S, Emmerich P, Ng WL, Schmitz H, Koay ESC. 2004. Evaluation of advanced reverse transcription-PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome-associated coronavirus. J Clin Microbiol 42:2043-2047.
7. Emery SL, Erdman DD, Bowen MD, Newton BR, Winchell JM, Meyer RF, Tong S, Cook BT, Holloway BP, McCaustland KA, Rota PA, Benkamp B, Lowe LE, Ksiazek TG, Bellini WJ, Anderson LJ. 2004. Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus. Emerg Infect Dis 10:311-316.
8. Fouchier RA, Kuiken T, Schutten M, van Amerongen G, van Doornum GJ, van den Hoogen BG, Peiris M, Lim W, Stohr K, Osterhaus AD. 2003. Aetiology: Koch's postulates fulfilled for SARS virus. Nature 423:240.
9. Ginocchio CC, Kemper M, Stellrecht KA, Witt DJ. 2003. Multicenter evaluation of the performance characteristics of the NucliSens HIV-1 QT assay used for quantitation of human immunodeficiency virus type 1 RNA. J Clin Microbiol 41:164-173.
10. Jiang SS, Chen TC, Yang JY, Hsiung CA, Su IJ, Liu YL, Chen PC, Juang JL. 2004. Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction. Clin Infect Dis 38:293-296.
11. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF, Ling AE, Humphrey CD, Shieh WJ, Guarner J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY, Cox N, Hughes JM, LeDuc JW, Bellini WJ, Anderson LJ. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 348:1953-1966.
12. Lanciotti RS, Kerst AJ. 2001. Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J Clin Microbiol 39:4506-4513.
13. Lau SK, Woo PC, Wong BH, Tsoi HW, Woo GK, Poon RW, Chan KH, Wei WI, Peiris JS, Yuen KY. 2004. Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay. J Clin Microbiol 42:2884-2889.
14. Mahony JB, Petrich A, Louie L, Song X, Chong S, Smieja M, Chernesky M, Loeb M, Richardson S. 2004. Performance and Cost evaluation of one commercial and six in-house conventional and real-time reverse transcription-PCR assays for detection of severe acute respiratory syndrome coronavirus. J Clin Microbiol 42:1471-1476.
15. Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, Cloutier A, Coughlin SM, Freeman D, Girn N, Griffith OL, Leach SR, Mayo M, McDonald H, Montgomery SB, Pandoh PK, Petrescu AS, Robertson AG, Schein JE, Siddiqui A, Smailus DE, Stott JM, Yang GS, Plummer F, Andonov A, Artsob H, Bastien N, Bernard K, Booth TF, Bowness D, Czub M, Drebot M, Fernando L, Flick R, Garbutt M, Gray M, Grolla A, Jones S, Feldmann H, Meyers A, Kabani A, Li Y, Normand S, Stroher U, Tipples GA, Tyler S, Vogrig R, Ward D, Watson B, Brunham RC, Krajden M, Petric M, Skowronski DM, Upton C, Roper RL. 2003. The Genome sequence of the SARS-associated coronavirus. Science 300:1399-1404.
16. Peiris JS, Chu CM, Cheng VC, Chan KS, Hung IF, Poon LL, Law KI, Tang BS, Hon TY, Chan CS, Chan KH, Ng JS, Zheng BJ, Ng WL, Lai RW, Guan Y, Yuen KY. 2003. Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: A prospective study. Lancet 361:1767-1772.
17. Poon LL, Chan KH, Wong OK, Cheung TK, Ng I, Zheng B, Seto WH, Yuen KY, Guan Y, Peiris JS. 2004. Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays. Clin Chem 50:67-72.
18. Poutanen SM, Low DE, Henry B, Finkelstein S, Rose D, Green K, Tellier R, Draker R, Adachi D, Ayers M, Chan AK, Skowronski DM, Salit I, Simor AE, Slutsky AS, Doyle PW, Krajden M, Petric M, Brunham RC, McGeer AJ. 2003. Identification of severe acute respiratory syndrome in Canada. N Engl J Med 348:1995-2005.
19. Rest JS, Mindell DP. 2003. SARS associated coronavirus has a recombinant polymerase and coronaviruses have a history of host-shifting. Infect Genet Evol 3:219-225.
20. Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R, Icenogle JP, Penaranda S, Bankamp B, Maher K, Chen MH, Tong S, Tamin A, Lowe L, Frace M, DeRisi JL, Chen Q, Wang D, Erdman DD, Peret TC, Burns C, Ksiazek TG, Rollin PE, Sanchez A, Liffick S, Holloway B, Limor J, McCaustland K, Olsen-Rasmussen M, Fouchier R, Gunther S, Osterhaus AD, Drosten C, Pallansch MA, Anderson LJ, Bellini WJ. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300:1394-1399.
21. Ruan YJ, Wei CL, Ee AL, Vega VB, Thoreau H, Su ST, Chia JM, Ng P, Chiu KP, Lim L, Zhang T, Peng CK, Lin EO, Lee NM, Yee SL, Ng LF, Ghee RE, Stanton LW, Long PM, Liu ET. 2003. Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection. Lancet 361:1779-1785.
22. World Health Organization Multicentre Collaborative Network for Severe Acute Respiratory Syndrome Diagnosis. 2003. A multicentre collaboration to investigate the cause of severe acute respiratory syndrome. Lancet 361:1730-1733.
23. Wu HS, Chiu SC, Tseng TC, Lin SF, Lin JH, Hsu YH, Wang MC, Lin TL, Yang WZ, Ferng TL, Huang KH, Hsu LC, Lee LL, Yang JY, Chen HY, Su SP, Yang SY, Lin SY, Lin TH, Su IS. 2004. Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan. Emerg Infect Dis 10:304-310.
24. Yam WC, Chan KH, Poon LL, Guan Y, Yuen KY, Seto WH, Peiris JS. 2003. Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus. J Clin Microbiol 41:4521-4524.
25. Zhang F, van der Laar S, Zimmerman D, Sillekens P, Ginocchio CC. 2004. Real time detection of SARS related coronavirus using nucleic acid sequence based amplification (NASBA) and molecular beacons. Abstract M10 20th Annual Clinical Virology Symposium, PASCV.