Subzero-temperature stabilization and spectroscopic characterization of homogeneous oxyferrous complexes of the cytochrome P450 BM3 (CYP102) oxygenase domain and holoenzyme

Biochemical and Biophysical Research Communications

Volumn 338, Issue 1, 2005, Pages 365-371

  Perera, Roshan ^a   Sono, Masanori ^a   Raner, Gregory M ^c   Dawson, John H ^a,b  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

^b UNIVERSITY OF SOUTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF NORTH CAROLINA AT GREENSBORO   (United States)

Author keywords

Cryoenzymology; Cysteinate ligated heme iron; Cytochrome P450 BM3; Dioxygen activation; Magnetic circular dichroism; Mono oxygenase; Oxyferrous heme iron

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 BM3; HEMOPROTEIN; HOLOENZYME; OXYGENASE; QUERCETIN; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; COMPLEX FORMATION; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME STABILITY; FREEZING; PRIORITY JOURNAL; PROTEIN DOMAIN; TEMPERATURE SENSITIVITY;

BACTERIAL PROTEINS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME STABILITY; FREEZING; IRON; MIXED FUNCTION OXYGENASES; OXYGEN; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY; THERMODYNAMICS;

EID: 27544513115     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.08.078     Document Type: Article

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