EPR and ENDOR of catalytic intermediates in cryoreduced native and mutant oxy-cytochromes P450cam: Mutation-induced changes in the proton delivery system [15]

Journal of the American Chemical Society

Volumn 121, Issue 45, 1999, Pages 10654-10655

  Davydov, Roman ^a   Macdonald, Iain D G ^b   Makris, Thomas M ^b   Sugar, Stephen G ^b   Hoffman, Brian M ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME P450;

CATALYSIS; CHEMICAL REACTION; LETTER; MUTATION; PROTON TRANSPORT;

EID: 0033579110     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9918829     Document Type: Letter

References (23)

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18. 35 GHz CW EPR and ENDOR spectra were recorded with a spectrometer of local design that employs 100 kHz field modulation and, for ENDOR, no frequency modulation. (Werst, M. W.; Davoust, C. E.; Hoffman, B. M. J. Am. Chem. Soc. 1991, 113, 1533-1538.)
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20. 60Co source with a dose of 3Mr as described (ref 13)(21) The spectrum for each protein shows a preponderant majority species but also one or more minority conformers. These are ignored here, but will be discussed in future work.
21. 60Co source with a dose of 3Mr as described (ref 13)(21) The spectrum for each protein shows a preponderant majority species but also one or more minority conformers. These are ignored here, but will be discussed in future work.
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