The expression of native and cultured human retinal pigment epithelial cells grown in different culture conditions

British Journal of Ophthalmology

Volumn 89, Issue 11, 2005, Pages 1510-1517

  Tian, J ^b   Ishibashi, K ^b   Honda, S ^c   Boylan, S A ^c   Hjelmeland, L M ^c   Handa, James T ^a,b  

^a JOHNS HOPKINS HOSPITAL   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; AGED; ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL STRAIN ARPE 19; CONTROLLED STUDY; CULTURE MEDIUM; GENE EXPRESSION; GENE FUNCTION; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOTOPE LABELING; NUCLEOTIDE SEQUENCE; PHENOTYPE; PIGMENT EPITHELIUM; PRIORITY JOURNAL; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA HYBRIDIZATION; RNA TRANSCRIPTION; UNINDEXED SEQUENCE;

AGED; AGED, 80 AND OVER; CELL CULTURE TECHNIQUES; CELL LINE; CLUSTER ANALYSIS; CULTURE MEDIA; CULTURE MEDIA, SERUM-FREE; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; MICRODISSECTION; MIDDLE AGED; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PIGMENT EPITHELIUM OF EYE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 27344455035     PISSN: 00071161     EISSN: None     Source Type: Journal    
DOI: 10.1136/bjo.2005.072108     Document Type: Article

References (13)

1. Dunn KC, Aotaki-Keen AE, Putkey FR, et al. ARPE-19, a human retinal pigment epithelial cell line with differentiated properties. Exp Eye Res 1996;62:155-69.
2. Tian J, Ishibashi K, Handa JT. The expression of native and cultured RPE grown on different matrices. Physiol Genomics 2004;17:170-82.
3. Johnston NL, Cervenak J, Shore AD, et al. Multivariate analysis of RNA levels from postmortem human brains as measured by three different methods of RT-PCR. Stanley Neuropathology Consortium. J Neurosci Methods 1997;77:83-92.
4. Barthel LK, Raymond PA. Improved method for obtaining 3-microns cryosections for immunocytochemistry. J Histochem Cytochem 1990;38:1383-8.
5. Boylan S, Honda S, Hjelmeland LM, et al. An optimized protocol for first strand cDNA synthesis from laser capture microdissected tissue. Lab Invest 2001;81:1167-9.
6. Sgroi DC, Teng S, Robinson G, et al. In vivo gene expression profile analysis of human breast cancer progression. Cancer Res 1999;59:5656-61.
7. Lee ML, Whitmore GA. Power and sample size for DNA microarray studies. Stat Med 2002;21:3543-70.
8. Alizadeh AA, Eisen MB, Davis RE, et al. Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 2000;403:503-11.
9. Weigel AL, Handa JT, Hjelmeland LM. Microarray analysis of H2O2-, HNE-, or tBH-treated ARPE-19 cells. Free Radic Biol Med 2002;33:1419-32.
10. Eisen MB, Spellman PT, Brown PO, et al. Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA 1998;95:14863-8.
11. Tusher VG, Tibshirani R, Chu G. Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA 2001;98:5116-21.
12. Simpson DA, Feeney S, Boyle C, et al. Retinal VEGF mRNA measured by SYBR green I fluorescence: a versatile approach to quantitative PCR. Mol Vis 2000;6:178-83.
13. Hwang D, Schmitt WA, Stephanopoulos G. Determination of minimum sample size and discriminatory expression patterns in microarray data. Bioinformatics 2002;18:1184-93.