An optimized protocol for first strand cDNA synthesis from laser capture microdissected tissue

Laboratory Investigation

Volumn 81, Issue 8, 2001, Pages 1167-1169

  Boylan, Sharon ^a   Honda, Shigeru ^a   Hjelmeland, Leonard M ^a   Handa, James T ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHROMOSOME MICRODISSECTION; DNA SYNTHESIS; LASER; MOLECULAR BIOLOGY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0034894151     PISSN: 00236837     EISSN: None     Source Type: Journal    
DOI: 10.1038/labinvest.3780329     Document Type: Article

References (16)

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3. Identification of differentially expressed genes from limited amounts of RNA
4. Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR
5. Laser capture microdissection
6. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue
7. The advanced glycation endproduct pentosidine induces the expression of PDGF-B in human retinal pigment epithelial cells
8. Carriers for precipitating nucleic acids
9. Analysis of anterior pituitary hormone mRNA expression in immunophenotypically characterized single cells after laser capture microdissection
10. Analysis of segmental renal gene expression by laser capture microdissection
11. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein
12. Reconstruction of bacteriophage T4 DNA replication apparatus from purified components: Rolling circle replication following de novo chain initiation on a single-stranded circular DNA template
13. Temporal expression of platelet-derived growth factor (PDGF)-A and its receptor in human preimplantation embryos
14. Quantitative determination of gene expression by competitive reverse transcription-polymerase chain reaction in degraded RNA samples
15. Enhanced detection of genetically engineered Corynebacterium glutamicum pUN1 in directly extracted DNA from soil, using the T4 gene 32 protein in the polymerase chain reaction