Rapid cloning and expression of glutaryl-7-aminocephalosporanic acid acylase genes from soil samples

Tsinghua Science and Technology

Volumn 10, Issue 5, 2005, Pages 529-534

  Luo, Hui ^a   Yu, Huimin ^a   Li, Qiang ^a   Shen, Zhongyao ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

Glutaryl 7 aminocephalosporanic acid acylase; Polymerase chain reaction; Pseudomonas; Soil

Indexed keywords

CLONING; DNA SEQUENCES; ESCHERICHIA COLI; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MICROORGANISMS; SOILS;

BIOINFORMATICS; DNA EXTRACTION; GENE ENCODING; GLUTARYL-7-AMINOCEPHALOSPORANIC ACID ACYLASE; POLYMERASE CHAIN REACTION (PCR); PSEUDOMONAS; SOIL SAMPLES;

ENZYMES;

EID: 26944433055     PISSN: 10070214     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1007-0214(05)70113-9     Document Type: Article

References (14)

1. Luo H, Li Q, Tong Y Z, Si Y T, Shen Z Y. Progress in enzymatic conversion of cephalosporin C to 7-aminoce-phalosporanic acid. Modern Chem. Industry, 2002, 22(12): 18-22. (in Chinese)
2. Kim D W, Yoon K H. Cloning and high expression of glutaryl 7-aminocephalosporanic acid acylase gene from Pseudomonas diminuta. Biotechnol. Lett., 2001, 23(13): 1067-1071.
3. Li Y, Chen J F, Jiang W H, Mao X, Zhao G P, Wang E D. In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130. Eur. Biochem., 1999, 262(3): 713-719.
4. Precigou S, Goulas P, Duran R. Rapid and specific identification of nitrile hydratase (NHase)-encoding genes in soil samples by polymerase chain reaction. FEMS Microbiol. Lett., 2001, 204(1): 155-161.
5. Cullen D W, Hirsch P R. Simple and rapid method for direct extraction of microbial DNA from soil for PCR. Soil Biol. Biochem., 1998, 30(8-9): 983-993.
6. Zhou J, Bruns A M, Tiedje J M. DNA recovery from soils of diverse composition. Appl. Environ. Microbiol., 1996, 62(2): 316-322.
7. Shibuya Y, Matsumoto K, Fujii T. Isolation and properties of 7β-(4-carboxybutanamido) cephalosporanic acid acylaseproducing bacteria. Agric. Biol. Chem., 1981, 45(7): 1561-1567.
8. Padmanabhan P, Shanker R, Khanna P. Short communication: A method for extraction of DNA and PCR-based detection of polycyclic aromatic hydrocarbon-degrading bacteria in soil contaminated with oil and grease. World J. Microbiol. Biotechnol., 1998, 14(6): 925-926.
9. Kim S, Kim Y. Active site residues of cephalosporin acylase are critical not only for enzymatic catalysis but also for post-translational modification. J. Biol. Chem., 2001, 276(51): 48376-48381.
10. Ishii Y, Saito Y, Fujimura Y, Isoga T, Kojo H, Yamashita M, Niwa M, Kohsak M. A novel 7-β-(4-carboxy butanamido)cephalosporanic acid acylase isolated from Pseudomonas strain C427 and its high-level production in Escherichia coli. J. Ferment. Bioeng., 1994, 77: 591-597.
11. Li Y, Jiang W, Yang Y, Zhao G, Wang E. Overproduction and purification of glutaryl 7-aminocephalosporanic acid acylase. Protein Expr. Purif., 1998, 12(2): 233-238.
12. Zheng Y G, Li Y, Chen J F, Jiang W H, Zhao G P, Wang E D. Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase. Biotechnol Lett., 2001, 23(21): 1781-1787.
13. Aramori I, Fukagawa M, Tsumura M, Iwami M, Yokota Y, Kojo H, Kohsaka M, Ueda Y, Imanaka H. Isolation of soil strains producing new cephalosporin acylases. J. Ferm. Bioeng., 1991, 72(4): 227-231.
14. Khang Y H, Yoo B H. Isolation and characterization of a novel soil strain. Pseudomonas cepacia BY21 with glutaryl-7-aminocephalosporanic acid acylase activity. Biotechnol. Lett., 2000, 22(4): 317-320.