Visualization of the trimeric P2X2 receptor with a crown-capped extracellular domain

Biochemical and Biophysical Research Communications

Volumn 337, Issue 3, 2005, Pages 998-1005

  Mio, Kazuhiro ^a   Kubo, Yoshihiro ^b,c,d   Ogura, Toshihiko ^a   Yamamoto, Tomomi ^b   Sato, Chikara ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES   (Japan)

^c TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^d JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

ATP; Electron microscopy; Ion channel; Negative staining; P2X receptor; Purinergic; Single particle analysis

Indexed keywords

PURINE P2X2 RECEPTOR; MULTIPROTEIN COMPLEX; PROTEIN SUBUNIT; PURINE P2 RECEPTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; CROSS LINKING; ELECTRON MICROSCOPY; GEL FILTRATION CHROMATOGRAPHY; IMAGING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SIGNAL NOISE RATIO; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; DIMERIZATION; EXTRACELLULAR SPACE; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; RAT; STRUCTURE ACTIVITY RELATION; ULTRASTRUCTURE;

ANIMALIA;

ANIMALS; DIMERIZATION; EXTRACELLULAR SPACE; MODELS, MOLECULAR; MULTIPROTEIN COMPLEXES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; RATS; RECEPTORS, PURINERGIC P2; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 26844438833     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.09.141     Document Type: Article

References (42)

1. R.A. North Molecular physiology of P2X receptors Physiol. Rev. 82 2002 1013 1067
2. F.A. Edwards, A.J. Gibb, and D. Colquhoun ATP receptor-mediated synaptic currents in the central nervous system Nature 359 1992 144 147
3. R.J. Evans, V. Derkach, and A. Surprenant ATP mediates fast synaptic transmission in mammalian neurons Nature 357 1992 503 505
4. J.G. Gu, and A.B. MacDermott Activation of ATP P2X receptors elicits glutamate release from sensory neuron synapses Nature 389 1997 749 753
5. B.S. Khakh, and G. Henderson ATP receptor-mediated enhancement of fast excitatory neurotransmitter release in the brain Mol. Pharmacol. 54 1998 372 378
6. F. Kato, and E. Shigetomi Distinct modulation of evoked and spontaneous EPSCs by purinoceptors in the nucleus tractus solitarii of the rat J. Physiol. 530 2001 469 486
7. A.J. Brake, M.J. Wagenbach, and D. Julius New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor Nature 371 1994 519 523
8. S. Valera, N. Hussy, R.J. Evans, N. Adami, R.A. North, A. Surprenant, and G. Buell A new class of ligand-gated ion channel defined by P2x receptor for extracellular ATP Nature 371 1994 516 519
9. B.S. Khakh, G. Burnstock, C. Kennedy, B.F. King, R.A. North, P. Seguela, M. Voigt, and P.P. Humphrey International union of pharmacology. XXIV. Current status of the nomenclature and properties of P2X receptors and their subunits Pharmacol. Rev. 53 2001 107 118
10. B.S. Khakh Molecular physiology of P2X receptors and ATP signalling at synapses Nat. Rev. Neurosci. 2 2001 165 174
11. L.H. Jiang, F. Rassendren, A. Surprenant, and R.A. North Identification of amino acid residues contributing to the ATP-binding site of a purinergic P2X receptor J. Biol. Chem. 275 2000 34190 34196
12. 2 receptor: estimates based on methanethiosulfonate block at T336C Mol. Pharmacol. 56 1999 973 981
13. 3 receptors form stable trimers: a novel structural motif of ligand-gated ion channels EMBO J. 17 1998 3016 3028
14. 1+2 receptor subtypes J. Mol. Biol. 342 2004 333 343
15. 6 receptor subunits do not oligomerize J. Biol. Chem. 280 2005 10759 10765
16. 2 receptor Eur. J. Pharmacol. 518 2005 107 110
17. A. Miyazawa, Y. Fujiyoshi, and N. Unwin Structure and gating mechanism of the acetylcholine receptor pore Nature 423 2003 949 955
18. N. Unwin Refined structure of the nicotinic acetylcholine receptor at 4 Å resolution J. Mol. Biol. 346 2005 967 989
19. W. Tichelaar, M. Safferling, K. Keinanen, H. Stark, and D.R. Madden The three-dimensional structure of an ionotropic glutamate receptor reveals a dimer-of-dimers assembly J. Mol. Biol. 344 2004 435 442
20. T. Nakagawa, Y. Cheng, E. Ramm, M. Sheng, and T. Walz Structure and different conformational states of native AMPA receptor complexes Nature 433 2005 545 549
21. E. Gouaux Structure and function of AMPA receptors J. Physiol. 554 2004 249 253
22. J. Frank Three-Dimensional Electron Microscopy of Macromolecular Assemblies 1996 Academic press San Diego
23. 2 receptor channel J. Physiol. 558 2004 31 43
24. P.K. Smith, R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson, and D.C. Klenk Measurement of protein using bicinchoninic acid Anal. Biochem. 150 1985 76 85
25. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
26. T. Ogura, and C. Sato Auto-accumulation method using simulated annealing enables fully automatic particle pickup completely free from a matching template or learning data J. Struct. Biol. 146 2004 344 358
27. T. Ogura, and C. Sato An automatic particle pickup method using a neural network applicable to low-contrast electron micrographs J. Struct. Biol. 136 2001 227 238
28. T. Ogura, and C. Sato Automatic particle pickup method using a neural network has high accuracy by applying an initial weight derived from eigenimages: a new reference free method for single-particle analysis J. Struct. Biol. 145 2004 63 75
29. T. Ogura, K. Iwasaki, and C. Sato Topology representing network enables highly accurate classification of protein images taken by cryo electron-microscope without masking J. Struct. Biol. 143 2003 185 200
30. M. van Heel, G. Harauz, E.V. Orlova, R. Schmidt, and M. Schatz A new generation of the IMAGIC image processing system J. Struct. Biol. 116 1996 17 24
31. W.O. Saxton, and W. Baumeister The correlation averaging of a regularly arranged bacterial cell envelope protein J. Microsc. 127 1982 127 138
32. 2 receptor subunit FEBS Lett. 425 1998 19 23
33. 2 receptor Biochemistry 37 1998 14845 14851
34. C. Sato, M. Sato, A. Iwasaki, T. Doi, and A. Engel The sodium channel has four domains surrounding a central pore J. Struct. Biol. 121 1998 314 325
35. K. Mio, T. Ogura, Y. Hara, Y. Mori, and C. Sato The non-selective cation-permeable channel TRPC3 is a tetrahedron with a cap on the large cytoplasmic end Biochem. Biophys. Res. Commun. 333 2005 768 777
36. C. Sato, Y. Ueno, K. Asai, K. Takahashi, M. Sato, A. Engel, and Y. Fujiyoshi The voltage-sensitive sodium channel is a bell-shaped molecule with several cavities Nature 409 2001 1047 1051
37. K. Keinanen, W. Wisden, B. Sommer, P. Werner, A. Herb, T.A. Verdoorn, B. Sakmann, and P.H. Seeburg A family of AMPA-selective glutamate receptors Science 249 1990 556 560
38. 3 receptor subunits can account for ATP-gated currents in sensory neurons Nature 377 1995 432 435
39. E.C. Burgard, W. Niforatos, T. van Biesen, K.J. Lynch, E. Touma, R.E. Metzger, E.A. Kowaluk, and M.F. Jarvis P2X receptor-mediated ionic currents in dorsal root ganglion neurons J. Neurophysiol. 82 1999 1590 1598
40. 2/3 receptor signaling in capsaicin-insensitive primary afferent neurons J. Neurosci. 20 RC90 2000 1 5
41. C. Virginio, A. MacKenzie, F.A. Rassendren, R.A. North, and A. Surprenant Pore dilation of neuronal P2X receptor channels Nat. Neurosci. 2 1999 315 321
42. 2 channel permeability by the cytosolic domain J. Gen. Physiol. 120 2002 119 131