Development of in vitro transposon assisted signal sequence trapping and its use in screening Bacillus halodurans C125 and Sulfolobus solfataricus P2 gene libraries

Journal of Microbiological Methods

Volumn 57, Issue 1, 2004, Pages 123-133

  Becker, Fiona ^a   Schnorr, Kirk ^a   Wilting, Reinhard ^a   Tolstrup, Niels ^b   Bendtsen, Jannick Dyrløv ^c   Olsen, Peter Bjarke ^a  

^a NOVOZYMES A S   (Denmark)

^b Exiqon AS   (Denmark)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Lac; Aa; Amino acids(s); Ampicillin; Ap; B.; Bacillus; Base pair; Beta lactamase; Bp; CAT; Chloramphenicol; Chloramphenicol acetyl transferase; Cm; E.; Escherichia; Industrial enzymes; Kanamycin; Km; Library screening; LSP; MuA transposon; Secreted; Signal trapping

Indexed keywords

BACTERIAL PROTEIN; BETA LACTAMASE; INDUSTRIAL ENZYME; MEMBRANE PROTEIN; SIGNAL PEPTIDE;

ARTICLE; BACILLUS; BACILLUS HALODURANS; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIOPHAGE MU; CONTROLLED STUDY; ESCHERICHIA COLI; GENE IDENTIFICATION; GENE LIBRARY; GENE SEQUENCE; GENE TARGETING; GENETIC SCREENING; GENOMICS; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROKARYOTE; PROTEIN DETERMINATION; PROTEIN DOMAIN; REPORTER GENE; SEQUENCE ANALYSIS; SEQUENCE TAGGED SITE; SULFOLOBUS SOLFATARICUS; TRANSPOSON; UNINDEXED SEQUENCE;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; BACTERIOPHAGE MU; BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; GENE LIBRARY; GENES, ARCHAEAL; GENES, BACTERIAL; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; SULFOLOBUS;

ARCHAEA; BACILLUS HALODURANS; BACTERIA (MICROORGANISMS); ENTEROBACTERIA PHAGE MU; ESCHERICHIA; ESCHERICHIA COLI; PROKARYOTA; SULFOLOBUS SOLFATARICUS;

EID: 1542375199     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2003.12.002     Document Type: Article

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