Exposure of nuclear antigens in formalin-fixed, paraffin-embedded necropsy human spinal cord tissue: Detection of NeuN

Journal of Neuroscience Methods

Volumn 148, Issue 1, 2005, Pages 26-35

  Gill, Sandeep K ^a,b   Ishak, Margaret ^b   Rylett, R Jane ^a,b  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

^b ROBARTS RESEARCH INSTITUTE   (Canada)

Author keywords

Antigen retrieval; Formalin fixed; immunofluorescence; Immunohistochemistry; Necropsy human neural tissue; NeuN; Nuclear proteins; Paraffin embedded

Indexed keywords

BUFFER; CELL NUCLEUS ANTIGEN; CHLORIDE; EPITOPE; FORMALDEHYDE; LIPOFUSCIN; NUCLEAR PROTEIN; PARAFFIN; PHENYLENEDIAMINE;

ANIMAL TISSUE; ANTIGEN RETRIEVAL; ARTICLE; AUTOPSY; CELL COMPARTMENTALIZATION; CELL NUCLEUS; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; METHODOLOGY; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN EXPRESSION; RAT; SPINAL CORD; TISSUE FIXATION;

ANIMALS; ANTIGENS, NUCLEAR; AZO COMPOUNDS; BORATES; COLORING AGENTS; FIXATIVES; FORMALDEHYDE; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOHISTOCHEMISTRY; INDOLES; MICE; NECROSIS; NERVE TISSUE PROTEINS; PARAFFIN EMBEDDING; PHOSPHOPYRUVATE HYDRATASE; RATS; SPINAL CORD; TISSUE FIXATION;

EID: 26444559465     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2005.03.008     Document Type: Article

References (31)

1. W. Baschong, R. Suetterlin, and R.H. Laeng Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM) J Histochem Cytochem 49 2001 1565 1572
2. J.H. Beckstead A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues: addendum J Histochem Cytochem 43 1995 345
3. P. Evers, and H.B. Uylings Microwave-stimulated antigen retrieval is pH and temperature dependent J Histochem Cytochem 42 1994 1555 1563
4. P. Evers, H.B. Uylings, and A.J. Suurmeijer Antigen retrieval in formaldehyde-fixed human brain tissue Methods 15 1998 133 140
5. T. Ezaki Antigen retrieval on formaldehyde-fixed paraffin sections: its potential drawbacks and optimization for double immunostaining Micron 31 2000 639 649
6. C.M. Ferrier, W.L. van Geloof, H.H. de Witte, M.D. Kramer, D.J. Ruiter, and G.N. van Muijen Epitopes of components of the plasminogen activation system are re-exposed in formalin-fixed paraffin sections by different retrieval techniques J Histochem Cytochem 46 1998 469 476
7. D. Gorlich Transport into and out of the cell nucleus EMBO J 17 1998 2721 2727
8. E. Kiseleva, M.W. Goldberg, J. Cronshaw, and T.D. Allen The nuclear pore complex: structure, function, and dynamics Crit Rev Eukaryot Gene Expr 10 2000 101 112
9. N.R. Krenz, and L.C. Weaver Sprouting of primary afferent fibers after spinal cord transection in the rat Neuroscience 85 1998 443 458
10. A.S. Leong, J. Milios, and C.G. Duncis Antigen preservation in microwave-irradiated tissues: a comparison with formaldehyde fixation J Pathol 156 1988 275 282
11. A.S. Leong, E.S. Lee, H. Yin, M. Kear, Z. Haffajee, and D. Pepperall Superheating antigen retrieval Appl Immunohistochem Mol Morphol 10 2002 263 268
12. A.S. Leong, H. Yin, and Z. Hafajee Patterns of immunoglobulin staining in paraffin-embedded malignant lymphomas Appl Immunohistochem Mol Morphol 10 2002 110 114
13. D. Lind, S. Franken, J. Kappler, J. Jankowski, and K. Schilling Characterization of the neuronal marker NeuN as a multiply phosphorylated antigen with discrete subcellular localization J Neurosci Res 79 2005 295 302
14. R.T. Miller, P.E. Swanson, and M.R. Wick Fixation and epitope retrieval in diagnostic immunohistochemistry: a concise review with practical considerations Appl Immunohistochem Mol Morphol 8 2000 228 235
15. J.D. Moore The Ran-GTPase and cell-cycle control Bioessays 23 2001 77 85
16. R.J. Mullen, C.R. Buck, and A.M. Smith NeuN a neuronal specific nuclear protein in vertebrates Development 116 1992 201 211
17. M. Neumann, and D. Gabel Simple method for reduction of autofluorescence in fluorescence microscopy J Histochem Cytochem 50 2002 437 439
18. Y. Onodera, H. Shimizu, S. Yamashita, and T. Nishikawa Cryofixed, freeze-dried and paraffin-embedded skin enables successful immunohistochemical staining of skin basement membrane antigens Histochemistry 98 1992 87 91
19. H.J. Romijn, J.F. van Uum, J. Emmering, V. Goncharuk, and R.M. Buijs Colocalization of VIP with AVP in neurons of the human paraventricular, supraoptic and suprachiasmatic nucleus. Brain Res 19 832 1999 47 53
20. FW.D. Rost Fluorescence microscopy Pearse AGE Histochemistry, theoretical and applied 1980 Churchill Livingstone New York 346 378
21. S.R. Shi, B. Chaiwun, L. Young, R.J. Cote, and C.R. Taylor Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections J Histochem Cytochem 41 1993 1599 1604
22. S.R. Shi, R.J. Cote, and C.R. Taylor Antigen retrieval immunohistochemistry: past, present, and future J Histochem Cytochem 45 1997 327 343
23. S.R. Shi, R.J. Cote, and C.R. Taylor Antigen retrieval immunohistochemistry and molecular morphology in the year 2001 Appl Immunohistochem Mol Morphol 9 2001 107 116
24. S.R. Shi, R.J. Cote, and C.R. Taylor Antigen retrieval techniques: current perspectives J Histochem Cytochem 49 2001 931 937
25. S.R. Shi, S.A. Imam, L. Young, R.J. Cote, and C.R. Taylor Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies J Histochem Cytochem 43 1995 193 201
26. S.R. Shi, M.E. Key, and K.L. Kalra Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections J Histochem Cytochem 39 1991 741 748
27. F. Soylemezoglu, S. Onder, G.G. Tezel, and M. Berker Neuronal nuclear antigen (NeuN): a new tool in the diagnosis of central neurocytoma Pathol Res Pract 199 2003 463 468
28. E.J. Speel, A.H. Hopman, and P. Komminoth Amplification methods to increase the sensitivity of in situ hybridization: play card(s) J Histochem Cytochem 47 1999 281 288
29. H.K. Wolf, R. Buslei, R. Schmidt-Kastner, P.K. Schmidt-Kastner, T. Pietsch, O.D. Wiestler, and I. Bluhmke NeuN: a useful neuronal marker for diagnostic histopathology J Histochem Cytochem 44 1996 1167 1171
30. P.J. Yao, T.M. O'Herron, and P.D. Coleman Immunohistochemical characterization of clathrin assembly protein AP180 and synaptophysin in human brain Neurobiol Aging 24 2003 173 178
31. D. Yin Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores Free Radic Biol Med 21 1996 871 888