Antigen retrieval on formaldehyde-fixed paraffin sections: Its potential drawbacks and optimization for double immunostaining

Micron

Volumn 31, Issue 6, 2000, Pages 639-649

  Ezaki, T ^a  

^a KUMAMOTO UNIVERSITY   (Japan)

Author keywords

Antigen retrieval; Double immunostaining; Enzyme digestion; Fixation; Heat induced epitope retrieval; Immunohistochemistry; Paraffin section; Proliferating cell nuclear antigen

Indexed keywords

ACTIN; ANTIGEN; BROXURIDINE; COLLAGEN; CYCLINE; FORMALDEHYDE; KERATIN; PARAFFIN; PEPSIN A; VIMENTIN;

ANIMAL; ARTICLE; GERMFREE ANIMAL; HUMAN; IMAGE ENHANCEMENT; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; METABOLISM; METHODOLOGY; PEYER PATCH; RAT; THYMUS; VISCERA;

ACTINS; ANIMALS; ANTIGENS; BROMODEOXYURIDINE; COLLAGEN; FORMALDEHYDE; HUMANS; IMAGE ENHANCEMENT; IMMUNOHISTOCHEMISTRY; KERATINS; PARAFFIN EMBEDDING; PEPSIN A; PEYER'S PATCHES; PROLIFERATING CELL NUCLEAR ANTIGEN; RATS; SPECIFIC PATHOGEN-FREE ORGANISMS; THYMUS GLAND; VIMENTIN; VISCERA;

ANIMALIA;

EID: 0034116758     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(99)00064-5     Document Type: Article

References (43)

1. Abbondanzo S.L., Allred D.C., Lampkin S., Banks P.M. Enhancement of immunoreactivity among lymphoid malignant neoplasms in paraffin-embedded tissues by refixation in zinc sulfate-formalin. Arch. Pathol. Lab. Med. 115:1991;31-33.
2. Allison R.T., Best T. p53, PCNA and Ki-67 expression in oral squamous cell carcinomas: the vagaries of fixation and microwave enhancement of immunocytochemistry. J. Oral Pathol. Med. 27:1998;434-440.
3. Baas I.O., van den Berg F.M., Mulder J.W., Clement M.J., Slebos R.J., Hamilton S.R., Offerhaus G.J. Potential false-positive results with antigen enhancement for immunohistochemistry of the p53 gene product in colorectal neoplasms. J. Pathol. 178:1996;264-267.
4. Battifora H., Kopinski M. The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation. J. Histochem. Cytochem. 34:1986;1095-1100.
5. Brandtzaeg P. Studies on J chain and binding site for secretory component in circulating human B cells. Clin. Exp. Immunol. 25:1976;50-58.
6. Brandtzaeg P. Tissue preparation methods for immunohistochemistry. Bullock G.R., Petrusz P. Techniques in Immunocytochemistry. 1982;1-75 Academic Press, London.
7. Carayon P., Bord A. Identification of DNA-replicating lymphocyte subsets using a new method to label the bromo-deoxyuridine incorporated into the DNA. J. Immunol. Methods. 147:1992;225-230.
8. Curran R.C., Gregory J. The unmasking of antigen in paraffin sections of tissue by trypsin. Experientia. 33:1977;1400-1401.
9. Cattoretti G., Pileri S., Parravicini C., Becker M.H.G., Poggi S., Bifulco C., Key G., D'Amato L., Sabattini E., Feudale E., Reynolds F., Gerdes J., Rilke F. Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J. Pathol. 171:1993;83-98.
10. Ezaki T. Antigen retrieval: its significance and drawbacks in immunohistochemistry. Kaibogaku Zasshi (Acta Anatomica Nipponica). 71:1996;615-628. in Japanese with English abstract and legends.
11. Ezaki T., Matsuno K., Fujii H., Hayashi N., Miyakawa K., Ohmori J., Kotani M. A new approach for identification of rat lymphatic capillaries using a monoclonal antibody. Arch Histol. Cytol. 53:1990;77-86.
12. Ezaki T., Yao L., Matsuno K. The identification of proliferating cell nuclear antigen (PCNA) on rat tissue cryosections and its application to double immunostaining with other markers. Arch Histol. Cytol. 58:1995;103-115.
13. Fox C.H., Johnson F.B., Whiting J., Roller P.P. Formaldehyde fixation. J. Histochem. Cytochem. 33:1985;845-853.
14. Hopwood Å.D. Cell and tissue fixation 1972-1982. Histochem. J. 17:1985;389-442.
15. Hosokawa Y., Tsuchihashi Y., Mitsufuji S., Tokita K., Isetani K., Tani T., Ashihara T. Correlation of fixation HCl hydrolysis and proteolytic digestion in bromodeoxyuridine immunohistochemistry of human stomach labeled by extracorporial perfusion system. Acta Histochem. Cytochem. 23:1990;261-270.
16. Hsu S.M., Soban E. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J. Histochem. Cytochem. 30:1982;1079-1082.
17. Huang S.N. Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections. Lab Invest. 33:1975;88-95.
18. Kawai K., Umemura S., Tsutsumi Y. Antigen retrieval by heating treatment. Saibo (Cell). 26:1994;152-157. in Japanese.
19. Kawai K., Serizawa A., Hamana T., Tsutsumi Y. Heat-induced antigen retrieval of proliferating cell nuclear antigen and p53 protein in formalin-fixed, paraffin-embedded sections. Pathol. Int. 44:1994;759-764.
20. Kitamoto T., Ogomori K., Tateishi J., Prusiner S.B. Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab. Invest. 57:1987;230-236.
21. Kodama T., Kawamoto K., Kono T., Shibuya Y., Setogawa T. Comparison of immunostainings for proliferating cell nuclear antigen and Ki-67 in human extraocular lesions. Graefes Arch Clin. Exp. Ophthalmol. 235:1997;767-772.
22. Kosaka T., Nagatsu I., Wu J.-Y., Hama K. Use of high concentration of glutaraldehyde for immunocytochemistry of transmitter-synthesizing enzymes in the central nervous system. Neuroscience. 18:1986;975-990.
23. Leong A.S.-Y., Gilham P.N. The effects of progressive formaldehyde fixation on the preservation of tissue antigens. Pathology. 21:1989;266-268.
24. Magi-Galluzzi C., Montironi R., Prete E., Kwan P.W., Delellis R.A. Effect of microwave oven heating times on androgen receptor antigen retrieval from paraffin-embedded prostatic adenocarcinoma. Anticancer Res. 16:1996;2931-2936.
25. McNicol A.M., Richmond J.A. Optimizing immunohistochemistry: antigen retrieval and signal amplification. Histopathology. 32:1998;97-103.
26. Mukai M., Torikata C., Iri H., Hata J., Naito M., Shimoda T. Immunohistochemical identification of aggregated actin filaments in formalin-fixed, paraffin-embedded sections. I. A study of infantile digital ibromatosis by a new pretreatment. Am. J. Surg. Pathol. 16:1992;110-115.
27. Nyhlin N., el-Salhy M., Sandstrom O., Suhr O. Evaluation of immunohistochemical staining of human duodenal endocrine cells after microwave antigen retrieval. Histochem. J. 29:1997;177-181.
28. Pearse A.G.E. The chemistry and practice of fixation. 1980;Churchill Livingstone, Edinburgh.
29. Reading M. A digestion technique for the reduction of background staining in the immunoperoxidase method. J. Clin. Pathol. 30:1977;88-90.
30. Reynolds G.M., Young F.I., Young J.A., Williams A., Rowlands D.C. Microwave oven antigen retrieval applied to the immunostaining of cytopathology specimens. Cytopathology. 5:1994;345-358.
31. Sebenik M., Wieczorek R. Nonspecific nuclear staining (NNS) after antigen retrieval (AR). Lab. Investigation. 72:1995;168A.
32. Shi S.-R., Key M.E., Kalra K.L. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39:1991;741-748.
33. Shi S.-R., Cote C., Kalra K.L., Taylor C.R., Tandon A.K. A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J. Histochem. Cytochem. 40:1992;787-792.
34. Shi S.-R., Gu J., Kalra K.L., Chen T., Cote R.J., Taylor C.R. Antigen retrieval technique: A novel approach to immunohistochemistry on routinely processed tissue sections. Cell Vision. 2:1995;6-23.
35. Shi S.-R., Cote R.J., Taylor C.R. Antigen retrieval immunohistochemistry: past, present, and future. J. Histochem. Cytochem. 45:1997;327-343.
36. Shin R.-W., Iwaki T., Kitamoto T., Tateishi J. Hydrated autoclave pretreatment enhances TAU immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissues. Lab Invest. 64:1991;693-702.
37. Shin H.J., Shin D.M., Shah T., Ro J.Y. Methods in pathology. Optimization of proliferating cell nuclear antigen immunohistochemical staining by microwave heating in zinc sulfate solution. Mod. Pathol. 7:1994;242-248.
38. Shintaku I.P., Said J.W. Detection of estrogen receptors with monoclonal antibodies in routinely processed formalin-fixed paraffin sections of breast carcinoma. Use of DNase pretreatment to enhance sensitivity of the reaction. Am. J. Clin. Pathol. 87:1987;161-167.
39. Stein H., Gatter K., Asbahr H., Mason D.Y. Use of freeze-dried paraffin-embedded sections for immunohistologic staining with monoclonal antibodies. Lab. Invest. 52:1985;676-683.
40. Suurmeijer A.J.H., Boon M.E. Notes on the application of microwaves for antigen retrieval in paraffin and plastic tissue sections. Eur. J. Morphol. 31:1993;144-150.
41. Suzuki S., Iwai M., Tome Y., Furuya S., Mukai K. Antigen retrieval of formalin-fixed, paraffin-embedded sections by microwave treatment. Byori To Rinsho (Pathology and Clinic). 10:1992;1191-1197. in Japanese.
42. Volkin D.B., Klibanov A.M. Minimizing protein inactivation. Creighton T.E. Protein function. A practical approach. 1989;1-24 IRL Press, Oxford.
43. Werner M., Von Wasielewski R., Komminoth P. Antigen retrieval, signal amplification and intensification in immunohistochemistry. Histochem. Cell Biol. 105:1996;253-260.