Chemical synthesis of the RGD-protein decorsin: Pro→Ala replacement reduces protein thermostability

Protein Engineering, Design and Selection

Volumn 18, Issue 10, 2005, Pages 487-495

  Frare, Erica ^a   De Laureto, Patrizia Polverino ^a   Scaramella, Elena ^a   Tonello, Fiorella ^a   Marin, Oriano ^a   Deana, Renzo ^a   Fontana, Angelo ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

Circular dichroism; Decorsin; Peptide synthesis; RGD protein; Thermal stability

Indexed keywords

ASSAYS; ENZYMES; OXIDATION; PATTERN RECOGNITION; SYNTHESIS (CHEMICAL); THERMODYNAMIC STABILITY;

CIRCULAR DIOCHROISM; DECORSIN; PEPTIDE SYNTHESIS; RGD-PROTEIN;

PROTEINS;

ALANINE; ARGINYLGLYCYLASPARTIC ACID; DECORSIN; DISULFIDE; POLYPEPTIDE; PROLINE; PROTEINASE K; THERMOLYSIN; UNCLASSIFIED DRUG;

ARTICLE; CIRCULAR DICHROISM; CROSS LINKING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEIN SYNTHESIS; STATISTICAL SIGNIFICANCE; THERMOSTABILITY; THROMBOCYTE AGGREGATION;

ALANINE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BLOOD PLATELETS; CIRCULAR DICHROISM; DRUG STABILITY; HEAT; HUMANS; INHIBITORY CONCENTRATION 50; PEPTIDE MAPPING; PLATELET AGGREGATION INHIBITORS; PROLINE; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 26444553312     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzi054     Document Type: Article

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