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Volumn 108, Issue 20, 2004, Pages 6485-6497

Micelles of lipid-oligonucleotide conjugates: Implications for membrane anchoring and base pairing

Author keywords

[No Author keywords available]

Indexed keywords

ABSORPTION SPECTROSCOPY; CELL MEMBRANES; DNA; ELECTRON MICROSCOPY; EMISSION SPECTROSCOPY; FLUORESCENCE; LIPIDS; MELTING; OLIGOMERS; SYNTHESIS (CHEMICAL); ULTRAVIOLET SPECTROSCOPY;

EID: 2642510833     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp031188m     Document Type: Article
Times cited : (51)

References (88)
  • 21
    • 0542418643 scopus 로고
    • (b) Fendler, J. Science 1984, 223, 888-894.
    • (1984) Science , vol.223 , pp. 888-894
    • Fendler, J.1
  • 66
    • 0001579306 scopus 로고
    • Fluorescence correlation spectroscopy
    • Lakowicz, J. R., Ed.; Plenum Press: New York
    • (c) For a comprehensive review: Thompson, N. L. Fluorescence Correlation Spectroscopy in Topics in Fluorescence Spectroscopy, Volume 1; Lakowicz, J. R., Ed.; Plenum Press: New York, 1991; pp 337-378.
    • (1991) Topics in Fluorescence Spectroscopy , vol.1 , pp. 337-378
    • Thompson, N.L.1
  • 70
    • 2642511243 scopus 로고    scopus 로고
    • note
    • In the present paper, the association constants are adimensional referring to the standard state: ideal solute at 1 M for the infinitely diluted solution.
  • 71
    • 0002413436 scopus 로고
    • Sinanoglu, O., Ed.; Academic Press: New York
    • Förster, T. Modern Quantum Chemistry; Sinanoglu, O., Ed.; Academic Press: New York, 1965; Part III, pp 93-137.
    • (1965) Modern Quantum Chemistry , Issue.PART III , pp. 93-137
    • Förster, T.1
  • 73
    • 2642525881 scopus 로고    scopus 로고
    • note
    • Their morphology was not further investigated; unfortunately, satisfactory EM pictures of these objects could not be obtained.
  • 77
    • 2642532548 scopus 로고    scopus 로고
    • note
    • 8(λ = 260 nm) = 0.25. Upon assuming the latter ratio to lie in the same range for the chalcone conjugated to the oligonucleotide, one anticipates the fluorophore contribution to the absorption of IGeB and IGeB′ at 260 nm to be less than 5% so as to justify to base the concentration determination on the DNA contribution only.
  • 84
    • 0029953291 scopus 로고    scopus 로고
    • See for instance: Jullien, L.; Canceill, J.; Valeur, B.; Bardez, E.; Lefèvre, J.-P.; Lehn, J.-M.; Marchi-Artzner, V.; Pansu, R. J. Am. Chem. Soc. 1996, 118, 5432-5442. In fact, eq 9 requires that the total concentration in lDNA remains constant during the titration to be valid. Under the present experimental conditions, the dilution is too large to consider this assumption to be fulfilled. Consequently, fluorescence intensities were corrected from dilution before analysis. Nevertheless, this correction is not sufficient to account for the change of association extent upon changing the lDNA concentration. Therefore, eq 9 was first used to derive orders of magnitude for the association constant and for the alteration of the lDNA emission properties. Then the latter estimates were further refined from numerical simulations.
    • (1996) J. Am. Chem. Soc. , vol.118 , pp. 5432-5442
    • Jullien, L.1    Canceill, J.2    Valeur, B.3    Bardez, E.4    Lefèvre, J.-P.5    Lehn, J.-M.6    Marchi-Artzner, V.7    Pansu, R.8
  • 85
    • 2642556921 scopus 로고    scopus 로고
    • note
    • In a first step, it was taken equal to the starting concentration during the titration. See ref 54.
  • 88
    • 2642565016 scopus 로고    scopus 로고
    • note
    • 2-HCl pH 7 buffer. We supposed that BOBO3 may be hydrolyzed under such conditions. We measured the rate constants for BOBO3 degradation at different temperatures in the absence or in the presence of C/G. Results are given in Supporting Information, Figure 7S and Table 2S. They essentially reveal that (i) DNA protects BOBO3 against decomposition; (ii) BOBO3 degradation remains slow at the time scale of the present assay so as to neglect it for interpreting experimental data.


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