Interaction, lipid exchange, and effect of vesicle size in systems of oppositely charged vesicles

Journal of Physical Chemistry

Volumn 100, Issue 32, 1996, Pages 13844-13856

  Marchi Artzner, Valérie ^a   Jullien, Ludovic ^a,b   Belloni, Luc ^c   Raison, Danièle ^d   Lacombe, Liliane ^a   Lehn, Jean Marie ^a  

^a l'alimentation et l'Environnement   (France)

^b ECOLE NORMALE SUPÉRIEURE   (France)

^c CEA SACLAY   (France)

^d SORBONNE UNIVERSITÉ   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000460872     PISSN: 00223654     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp960327f     Document Type: Article

References (64)

1. Abbreviations: CC, charged component; Chol, cholesterol; DOD-AB, dioctadecylammonium bromide; DPA, dipicolinic acid; EPC, egg yolk lecithin; LUV, large unilamellar vesicles; NBD-PE, N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) phosphatidylethanolamine; RET, resonance energy transfer; REV, vesicles prepared by the reverse phase method; Rh-PE, N-(Lissamine rhodamine B sulfonyl)phosphatidylethanolamine; TNS, 2-(p-toluidino)-naphthalene-6-sulfonic acid.
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52. By taking into account average vesicular diameters and the respective internal cesium concentrations of initially negatively and positively charged vesicles, one should expect by fusion a new NMR peak at about 1.4 ppm. For the purpose of clarity, the interpretation of the peak at 1.18 ppm will be given in the following paragraphs.
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56. Some remaining detergent should have played a role in the exchange process. In fact, we did not detect any significant amount of octylglucopyranoside, if any, during analysis of membrane composition. Furthermore, we reproduced Rh-PE and TNS titrations with REV prepared in the absence of detergent and DD LUV-like observations were made.
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58. The following discussion assumes that fluorescent probes do not induce any change of the mechanism of vesicle interaction. All experiments are thus expected to provide a coherent picture.
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61. Indeed, phosphate lipids are expected to transport cations through bilayers by flip-flop of the neutral ion pair. The difference in behavior between positively and negatively charged vesicles in the leakage rate is explained since it is known that cation exit constitutes the rate-limiting step of passive leakage of salts contained in vesicles.
62. The ratio of compartmented volumes is proportional to the ratio of vesicle diameters since lipid concentrations and vesicle structures are identical for both populations of vesicles.
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64. The decreasing branch of the titration curve is explained by considering that already one-time neutralized vesicles become neutral after subsequent collisions with the positively charged titrating vesicles. Figure 4 suggests the Rh-PE fluorescence emission in EPC or negatively charged vesicles to be similar. Nevertheless, one cannot exclude some marginal EPC hydrolysis to yield a negatively charged surface. In a true neutral environment, Rh-PE should give an intermediate signal between positively and negatively charged vesicles.