Biochemistry: Rev1 employs a novel mechanism of DNA synthesis using a protein template

Science

Volumn 309, Issue 5744, 2005, Pages 2219-2222

  Nair, Deepak T ^a   Johnson, Robert E ^b   Prakash, Louise ^b   Prakash, Satya ^b   Aggarwal, Aneel K ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS MEDICAL BRANCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL STRUCTURE; ENZYMES; HYDROGEN BONDS; SYNTHESIS (CHEMICAL); YEAST;

ERROR FREE SYNTHESIS; POLYMERASE;

DNA;

ARGININE; CYTOSINE; DEOXYCYTIDINE TRIPHOSPHATE; DNA; DNA BASE; DNA POLYMERASE; DNA POLYMERASE REV1; GUANINE; NUCLEOTIDE; UNCLASSIFIED DRUG; 2'-DEOXYCYTIDINE 5'-TRIPHOSPHATE; DEOXYCYTIDINE PHOSPHATE; FUNGAL DNA; HYBRID PROTEIN; NUCLEOTIDYLTRANSFERASE; REV1 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN;

BIOCHEMISTRY;

ARTICLE; BASE PAIRING; CRYSTAL STRUCTURE; DNA ADDUCT; DNA HELIX; DNA REPLICATION; DNA SYNTHESIS; ENZYME SPECIFICITY; ENZYME STRUCTURE; HYDROGEN BOND; PRECURSOR; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN TEMPLATE; BINDING SITE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; DNA TEMPLATE; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; HYDROPHOBICITY; METABOLISM; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SACCHAROMYCES CEREVISIAE; X RAY CRYSTALLOGRAPHY;

ARGININE; BASE PAIRING; BINDING SITES; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DEOXYCYTOSINE NUCLEOTIDES; DNA REPLICATION; DNA, FUNGAL; GUANINE; HYDROGEN BONDING; HYDROPHOBICITY; MODELS, MOLECULAR; NUCLEOTIDYLTRANSFERASES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TEMPLATES, GENETIC;

EID: 25844440534     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1116336     Document Type: Article

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27. Single-letter abbreviations for the amino acid residues here are as follows D, Asp; E, Glu; F, Phe; G, Gly; K, Lys; L, Leu; M, Met; R, Arg; S, Ser; W, Trp; and Y, Tyr.
28. We thank the staff at Brookhaven National Laboratory (beamline X6A) and Advanced Photon Source (beamline 17ID) for facilitating X-ray data collection. We thank C. Escalante, V. Fedorov, S. Lone, and L. Shen for general assistance. This work was supported by grants from the NIH (A.K.A, S.P, and L.P.). The structure has been deposited in the Protein Data Bank with the accession number 2AQ4.