A platform for high-throughput expression of recombinant human enzymes secreted by insect cells

Journal of Biotechnology

Volumn 120, Issue 1, 2005, Pages 59-71

  Redaelli, Loredana ^a   Zolezzi, Francesca ^a   Nardese, Vanessa ^a   Bellanti, Beatrice ^a   Wanke, Valeria ^a,b   Carettoni, Daniele ^a  

^a Axxam   (Italy)

^b UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

Baculovirus; High throughput; Insect cells; Lipases; Peptidases; Secreted proteins

Indexed keywords

BIOASSAY; CELLS; FLUORESCENCE; GENES; GROWTH KINETICS; PROTEINS;

BACULOVIRUS; HIGH-THROUGHPUT; INSECT CELLS; LIPASES; PEPTIDASES; SECRETED PROTEINS;

ENZYME KINETICS;

PEPTIDASE; POLYHISTIDINE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; ANIMAL CELL; BACULOVIRUS; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CELL CULTURE; CELL GROWTH; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME RELEASE; FLUORESCENCE ANALYSIS; FUNCTIONAL GENOMICS; GENE EXPRESSION; GENE FUNCTION; GENE SEQUENCE; HOST CELL; HUMAN GENOME; INSECT CELL; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PILOT STUDY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEOMICS;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; ENZYMES; GENETIC ENHANCEMENT; HUMANS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SPODOPTERA; TRANSFECTION;

INSECTA;

EID: 25644446195     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.05.026     Document Type: Conference Paper

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