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note
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Two different isomers (syn/anti) of 3-oxime compound were synthesized when RU-486 was refluxed with hydroxylamine hydrochloride in pyridine. The isomers were separated by column chromatography on silica using the eluent of hexane:ethyl acetate = 1:1.
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note
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Eliminating isomerism (syn/anti at C3) may enhance the binding affinity. We attempted to separate the isomeric mixture to determine (if any) the effect that a purified isomer may have; however, we were unsuccessful.
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