In vivo visualization of gene expression using magnetic resonance imaging

Nature Biotechnology

Volumn 18, Issue 3, 2000, Pages 321-325

  Louie, Angelique Y ^a   Hüber, Martina M ^a,b   Ahrens, Eric T ^a   Rothbächer, Ute ^a,c   Moats, Rex ^a,d   Jacobs, Russell E ^a   Fraser, Scott E ^a   Meade, Thomas J ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b Chemetall GmbH   (Germany)

^c LGPD   (France)

^d CHILDREN S HOSPITAL LOS ANGELES   (United States)

Author keywords

Contrast agent; Gadolinium; Gene expression; LacZ; MRI; galactosidase

Indexed keywords

BETA GALACTOSIDASE;

ARTICLE; CONTRAST ENHANCEMENT; EMBRYO; GENE EXPRESSION; GENE MAPPING; IMAGE PROCESSING; IN VIVO STUDY; KINETICS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE IMAGING; PRIORITY JOURNAL; REPORTER GENE; XENOPUS;

ANIMALIA; EUKARYOTA;

EID: 0342445413     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/73780     Document Type: Article

References (36)

1. Davidson, E.H. Gene activity in early development, Edn. 3 (Academic Press, New York, NY; 1986).
2. Gerhart, J. & Kirschner, M. Cells embryos and evolution. (Blackwell, New York, NY; 1997).
3. Wilson, E.B. The cell in development and inheritance (Macmillan, New York, NY; 1986).
4. Ziokarnik, G. et al. Quantitäten of transcription and clonal selection of single living cells with beta-lactamase as reporter. Science 279, 84-88 (1998).
5. Nunez, L, Faught, W.J. & Frawley, L.S. Episodic gonadotropin-releasing hormone gene expression revealed by dynamic monitoring of luciferase reporter activity in single, living neurons. Proc. Natl. Acad. Sci. USA 95, 9648-9653 (1998).
6. Arnone, M. I. et al. Green fluorescent protein in the sea urchin: new experimental approaches to transcriptional regulatory analysis in embryos and larvae. Development 124, 4649-4659 (1997).
7. Chiu, W. et al. Engineered GFP as a vital reporter in plants. Curr. Biol. 6, 325-330 (1996).
8. Amsterdam, A.. Lin, S. & Hopkins, N. The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos. Dev. Biol. 171, 123-129 (1995).
9. Prasher, D. Using GFP to see the light. TIG 11, 320-323 (1995).
10. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. & Prasher, D. Green fluorescent protein as a marker for gene expression. Science 263, 802-805 (1994).
11. Tjuvajev, J. G. et al. Imaging the expression of transfected genes in vivo. Cancer Res. 55, 6126-6132 (1995).
12. Tjuvajev, J. G. et al. Noninvasive imaging of herpes virus thymidine kinase gene transfer and expressions potential method for monitoring clinical gene therapy. Cancer Res. 56, 4087-4095 (1996).
13. Gambhir, S. S. et al. Imaging adenoviral-directed reporter gene expression in living animals with positron emission tomography. Proc. Natl. Acad. Sci. USA 96, 2333-2338 (1999).
14. Li, W.H., Fraser, S.E. & Meade, T.J. A calcium-sensitive magnetic resonance imaging contrast agent. J. Am. Chem. Soc. 121, 1413-1414 (1999).
15. Bowtell, R. W. et al. NMR microscopy of single neurons using spin-echo and line-narrowed 2DFT imaging. Magn. Reson. Med. 33, 790-794 (1995).
16. Rote, C.T., Vannoort, J., Back, P.J. & Callaghan, P.T. NMR microscopy using large, pulsed magnetic-field gradients. J. Magn. Reson. B. 108, 125-136 (1995).
17. Mellin, A.F. et al. 3-Dimensional magnetic-resonance microangiography of rat neurovasculature. Mag. Reson. Med. 32, 199-205 (1994).
18. Liang, Z.P. & Lauterbur, P.C. An efficient method for dynamic magnetic-resonance imaging. IEEE Trans. Med. Imaging 13, 677-686 (1994).
19. Jacobs, R.E. & Fraser, S.E. Magnetic resonance microscopy of embryonic-cell lineages and movements. Science 263, 681-684 (1994).
20. Hueber, M.M. et al. Fluorescently detectable magnetic resonance imaging agents. Bioconjug. Chem. 9, 242-249 (1998).
21. Su, M.Y., Muhler, A., Lao, X.Y. & Nalcioglu, O. Tumor characterization with dynamic contrast-enhanced MRI using MR contrast agents of various molecular weights. Mag. Res. Med. 39, 259-269 (1998).
22. Aime, S., Botta, M., Fasano, M. & Terreno, E. Lanthanide(III) chelates for NMR biomedical applications. Chem. Soc. Rev. 27, 19-29 (1998).
23. Shukla, R. et al. Design of conformationally rigid dimeric MRI agents. Mag. Res. Med. 35, 928-931 (1996).
24. Bertini, I. & Luchinat, C. NMR of paramagnetic molecules in biological systems (eds Gray, H.B. & Lever, A.B.R) (Benjamin/Cummings, Menlo Park, CA; 1986).
25. Moore, A., Basilion, J.P., Chiocca, E.A. & Weissleder, R. Measuring transferrin receptor gene expression by NMR imaging. BBA 1402, 239-249 (1998).
26. Weissleder, R. et al. MR imaging and scintigraphy of gene expression through melanin induction. Radiology 204, 425-429 (1997).
27. Moats, R.A., Fraser, S.E. & Meade, T.J. A "smart" magnetic resonance imaging agent that reports on specific enzyme activity. Angew. Chem. Intl. Edn. Engl. 726-728 (1997).
28. Ahrens, E.T., Rothbacher, U., Jacobs, R.E. & Fraser, S.E. A model for MRI contrast enhancement using T1 agents. Proc. Natl. Acad. Sci. USA 95, 8443-8448 (1998).
29. Zhang, X. et al. pH dependence of relaxivities and hydration numbers of gadolinium(III) complexes of macrocyclic amino carboxylates. Inorg. Chem 31, 5597-5600 (1992).
30. Horrocks, W.D. & Sudnick, D.R. Lanthanide ion probes of structure in biology. Laser-induced luminescence decay constants provide a direct measure of the number of metal-coordinated water molecules. J. Am. Chem. Soc. 101, 334-340 (1979).
31. Wetts, R. & Fraser, S.E. Slow intermixing of cells during Xenopus embryogenesis contributes to the consistency of the blastomere fate map. Development 105, 9-15 (1989).
32. Kroll, K.L. & Amaya, E. Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation. Development 122, 3173-3183 (1996).
33. Kayyem, J.F., Kumar, R.M., Fraser, S.E. & Meade, T.J. Receptor-targeted cotransport of DNA and magnetic resonance contrast agents. Chem. Biol. 2, 615-620 (1995).
34. Bogdanov, A. & Weissleder, R. The development of in vivo imaging systems to study gene expression. Trends Biotech. 16, 5-10 (1998).
35. Josephson, L., Tung, C., Moore, A. & Weissleder, R. High-efficiency intracellular magnetic labeling with novel superparamagnetic-tat peptide conjugates. Bioconjug. Chem. 10, 186-191 (1999).
36. Kay, B.K., Peng, H.B., Methods in Cell Biology Vol.6 (Academic Press, NY, 1991).