The mechanical hierarchies of fibronectin observed with single-molecule AFM

Journal of Molecular Biology

Volumn 319, Issue 2, 2002, Pages 433-447

  Oberhauser, Andres F ^a   Badilla Fernandez, Carmelu ^a   Carrion Vazquez, Mariano ^a   Fernandez, Julio M ^a  

^a MAYO CLINIC   (United States)

Author keywords

AFM; Fibronectin; Hierarchies; Mechanical unfolding; Single molecule

Indexed keywords

FIBRONECTIN; POLYPROTEIN;

ARTICLE; ATOMIC FORCE MICROSCOPY; CHEMICAL PARAMETERS; EXTRACELLULAR MATRIX; FORCE; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STABILITY; SPECTROSCOPY; THERMODYNAMICS;

EID: 0036305982     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00306-6     Document Type: Article

References (56)

1. Mechanical properties of the extracellular matrix influence fibronectin fibril assembly in vitro
2. Unravelling the extracellular matrix
3. Fibronectin fibrillogenesis: A paradigm for extracellular matrix assembly
4. Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly
5. The dynamic dialogue between cells and matrices: Implications of fibronectin's elasticity
6. Fibronectin-integrin interactions
7. Crystal structure of a heparin- and integrin-binding segment of human fibronectin
8. Cryptic self-association sites in type III modules of fibronectin
9. Fibronectin's III-1 module contains a conformation-dependent binding site for the amino-terminal region of fibronectin
10. A novel fibronectin binding site required for fibronectin fibril growth during matrix assembly
11. Interactions between type III domains in the 110 kDa cell-binding fragment of fibronectin
12. Module-module interactions in the cell-binding region of fibronectin: Stability, flexibility and specificity
13. A comparison of the folding kinetics and thermodynamics of two homologous fibronectin type III modules
14. Folding of beta-sandwich proteins: Three-state transition of a fibronectin type III module
15. Forced unfolding of fibronectin type 3 modules: An analysis by biased molecular dynamics simulations
16. Unfolding proteins by external forces and temperature: The importance of topology and energetics
17. Forced unfolding of the fibronectin type III module reveals a tensile molecular recognition switch
18. Comparison of the early stages of forced unfolding for fibronectin type III modules
19. Reversible unfolding of individual titin immunoglobulin domains by AFM
20. The mechanical stability of immunoglobulin and fibronectin III domains in the muscle protein titin measured by atomic force microscopy
21. Single molecule force spectroscopy of spectrin repeats: Low unfolding forces in helix bundles
22. Unfolding forces of titin and fibronectin domains directly measured by AFM
23. The molecular elasticity of the extracellular matrix protein tenascin
24. Mechanical and chemical unfolding of a single protein: A comparison
25. Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering
26. Conformational analysis of native fibronectin by means of force spectroscopy
27. Solid-state synthesis and mechanical unfolding of polymers of T4 lysozyme
28. Unfolding pathways of individual bacteriorhodopsins
29. Single molecular force spectroscopy of modular proteins in the nervous system
30. Can non-mechanical proteins withstand force? Stretching barnase by atomic force microscopy and molecular dynamics simulation
31. Mechanical unfolding of single filamin A (ABP-280) molecules detected by atomic force microscopy
32. Molecular mechanics of cardiac titin's PEVK and N2B spring elements
33. Atomic force microscopy reveals the mechanical design of a modular protein
34. Strength of a weak bond connecting flexible polymer chains
35. Modeling AFM-induced PEVK extension and the reversible unfolding of Ig/FNIII domains in single and multiple titin molecules
36. 2.0 Å crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region
37. The effect of boundary selection on the stability and folding of the third fibronectin type III domain from human tenascin
38. Solution structure of the N-terminal F1 module pair from human fibronectin
39. Multiple conformations of PEVK proteins detected by single-molecule techniques
40. Stretching DNA
41. Point mutations alter the mechanical stability of immunoglobulin modules
42. The elastic I-band region of titin is assembled in a "modular" fashion by weakly interacting Ig-like domains
43. Models for the specific adhesion of cells to cells
44. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy
45. Fibronectin molecule visualized in electron microscopy: A long, thin, flexible strand
46. Keratocytes pull with similar forces on their dorsal and ventral surfaces
47. Keratocytes generate traction forces in two phases
48. Force and focal adhesion assembly: A close relationship studied using elastic micropatterned substrates
49. Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent
50. Coexisting conformations of fibronectin in cell culture imaged using fluorescence resonance energy transfer
51. Lifetime of the P-selectin-carbohydrate bond and its response to tensile force in hydrodynamic flow
52. The kinetics and shear threshold of transient and rolling interactions of L-selectin with its ligand on leukocytes
53. Structural insights into the mechanical regulation of molecular recognition sites
54. Reversible unfolding of fibronectin type III and immunoglobulin domains provides the structural basis for stretch and elasticity of titin and fibronectin
55. Sensing specific molecular-interactions with the atomic force microscope
56. Calibration of atomic-force microscope tips