Nanomechanical sensing of DNA sequences using piezoresistive cantilevers

Langmuir

Volumn 21, Issue 18, 2005, Pages 8400-8408

  Mukhopadhyay, R ^a   Lorentzen, M ^a   Kjems, J ^a   Besenbacher, F ^a  

^a AARHUS UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DETECTION ASSAYS; NANOMECHANICAL SENSING; PIEZORESISTIVE CANTILEVERS; SURFACE STRESS;

BIOASSAY; NANOSTRUCTURED MATERIALS; PIEZOELECTRIC DEVICES; REACTION KINETICS; SENSORS; SURFACE STRUCTURE;

DNA SEQUENCES;

DNA; NANOMATERIAL; OLIGONUCLEOTIDE; THIOL DERIVATIVE;

ARTICLE; CHEMISTRY; GENETIC PROCEDURES; INSTRUMENTATION; METHODOLOGY; NUCLEIC ACID PROBE; NUCLEOTIDE SEQUENCE; SCANNING ELECTRON MICROSCOPY;

BASE SEQUENCE; BIOSENSING TECHNIQUES; DNA; MICROSCOPY, ELECTRON, SCANNING; NANOSTRUCTURES; NUCLEIC ACID PROBES; OLIGONUCLEOTIDES; SULFHYDRYL COMPOUNDS;

EID: 24944439851     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la0511687     Document Type: Article

References (23)

1. Fritz, J.; Baller, M. K.; Lang, H. P.; Rothuizen, H.; Vettiger, P.; Meyer, E.; Güntherodt, H.-J.; Gerber, Ch.; Gimzewski, J. K. Science 2000, 288, 316-318.
2. Wu, G.; Ji, H.; Hansen, K.; Thundat, T.; Datar, R.; Cote, R.; Hagan, M. F.; Chakraborty, A. K.; Majumdar, A. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 1560-1564.
3. Hansen, K.; Ji, H.; Wu, G.; Datar, R.; Cote, R.; Majumdar, A.; Thundat, T. Anal. Chem. 2001, 73, 1567-1571.
4. McKendry, R. A.; Zhang, J.; Arntz, Y.; Strunz, T.; Hegner, M.; Lang, H. P.; Baller, M. K.; Certa, U.; Meyer, E.; Güntherodt, H.-J.; Gerber, Ch. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 9783-9788.
5. Boisen, A.; Thaysen, J.; Jensenius, H.; Hansen, O. Ultramicroscopy 2000, 82, 11-16.
6. Lutwyche, M. I.; Despont, M.; Drechsler, U.; Durig, U.; Häberle, W.; Rothuizen, H.; Stuz, R.; Widmer, R.; Binnig, G. K.; Vettiger, P. Appl. Phys. Lett. 2000, 77, 3299-3301.
7. Singh, S. K.; Nielsen, P.; Koshkin, A. A.; Wengel, J. Chem. Commun. 1998, 455-456.
8. Cantion A/S, Lyngby, Copenhagen, Denmark (http://www.cantion.com).
9. Kovacs, G. T. A. Micromachined Transducers Sourcebook, McGraw-Hill, New York, 1998.
10. Bietsch, A.; Zhang, J.; Hegner, M.; Lang, H. P.; Gerber, Ch. Nanotechnology 2004, 15, 873-880.
11. Bietsch, A.; Hegner, M.; Lang, H. P.; Gerber, Ch. Langmuir 2004, 20, 5119-5122.
12. Deprotection protocol: Suspend the oligo in 0.1 M triethylammonium acetate (TEAA) at pH 6.5 and a concentration of approximately 100 OD/mL. Add 0.15 volumes of 1 M aqueous silver nitrate solution, mix thoroughly, and leave to react at room temperature for 30 min. Add 0.20 volumes of 1 M aqueous dithiothreitol (DTT) solution, mix thoroughly, and leave at room temperature for 5 min. Centrifuge the suspension to remove the silver DTT complex. Remove the supernatant. Wash the precipitate with 1 volume of 0.1 M TEAA. Centrifuge and combine the supernatant with the first volume.
13. TCEP treatment protocol: Mix 100 μL of TCEP (50 mM at pH 7.0) to 20 μL of DNA (∼80 μM/μL) and keep the solution at room temperature for 30 min. Remove excess TCEP by running this solution through a sephadex G-25 microspin column by centrifugation. For exchange in a buffer, elute in the buffer using a similar column.
14. Smith, S. B.; Cui, Y.; Bustamante, C. Science 1996, 271, 795-799.
15. Baumann, C. G.; Smith, S. B.; Bloomfield, V. A.; Bustamante, C. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 6185-6190.
16. Herne, T. M.; Tarlov, M. J. J. Am. Chem. Soc. 1997, 119, 8916-8920.
17. Steel, A. B.; Levicky, R. L.; Herne, T. M.; Tarlov, M. J. Biophys. J. 2000, 79, 975-981.
18. Thaysen, J., Ph.D. Thesis, MIC, Technical University of Denmark, Denmark, 2001.
19. Personal communication, Thaysen, J., Cantion A/S, Denmark.
20. Hagan, M. F.; Majumdar, A.; Chakraborty, A. K. J. Phys. Chem. B 2002, 106, 10163-10173.
21. Safran, S. A. Adv. Phys. 1999, 48, 395-448.
22. Castelino, K.; Kannan, B.; Majumdar, A. Langmuir 2005, 21, 1956-1961.
23. Leiken, S.; Parsegian, V. A.; Rau, D. C.; Rand, R. P. Annu. Rev. Phys. Chem. 1993, 44, 369-395.