Homogeneous tetracycline-regulatable gene expression in mammalian fibroblasts

Journal of Cellular Biochemistry

Volumn 76, Issue 2, 2000, Pages 280-289

  Izumi, Masako ^a   Gilbert, David M ^b  

^a INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^b SUNY UPSTATE MEDICAL UNIVERSITY   (United States)

Author keywords

CHO cells; Gene expression; GFP; Tetracycline; Transfection

Indexed keywords

TETRACYCLINE;

ANIMAL CELL; ARTICLE; CELL FRACTIONATION; CHO CELL; CONTROLLED STUDY; FIBROBLAST; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE;

EID: 0033452061     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(20000201)76:2<280::AID-JCB11>3.0.CO;2-0     Document Type: Article

References (15)

1. Blau HM, Rossi FM. 1999. Tet B or not tet B: advances in tetracycline-inducible gene expression [comment]. Proc Natl Acad Sci USA 96:797-799.
2. Chung JH, Whiteley M, Felsenfeld G. 1993. A 5′ element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. Cell 74:505-514.
3. Forster K, Helbl V, Lederer T, Urlinger S, Wittenburg N, Hillen W. 1999. Tetracycline-inducible expression systems with reduced basal activity in mammalian cells. Nucleic Acids Res 27:708-710.
4. Gilbert D, Losson R, Chambon P. 1992. Ligand dependence of estrogen receptor induced changes in chromatin structure. 17:4525-4531.
5. Gilbert DM. 1989. Temporal order of DNA replication in eukaryotic cells: its relationship to gene expression. In: Genetics. Stanford, CA: Stanford University Press.
6. Gossen M, Bujard H. 1992. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 89:5547-5551.
7. Hamlin JL, Mosca PJ, Levenson UV. 1994. Defining origins of replication in mammalian cells. Biochim Biophys Acta 1198:85-111.
8. Izumi M, Miyazawa H, Kamakura T, Yamaguchi I, Endo T, Hanaoka F. 1991. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells [published erratum appears in Exp Cell Res 1993 Feb;204(2):388]. Exp Cell Res 197:229-233.
9. Jiang W, Hunter T. 1998. Analysis of cell-cycle profiles in transfected cells using a membrane-targeted GFP. Bio-Techniques 24:348-354.
10. MacGregor GR, Mogg AE, Burke JF, Caskey CT. 1987. Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of the bacterial gene. Somat Cell Mol Genet 13:253-265.
11. Pikaart MJ, Recillas-Targa F, et al. 1998. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Genes Dev 12:2852-2862.
12. Resnitsky D, Reed S. 1995. Different roles for cyclins D1 and E in regulation of the G1-to-S transition. Mol Cell Biol 15:3463-3469.
13. Shockett PE, Schatz DG. 1996. Diverse strategies for tetracycline-regulated inducible gene expression [see comments]. Proc Natl Acad Sci USA 93:5173-5176.
14. van Steensel B, de Lange T. 1997. Control of telomere length by the human telomeric protein TRF1 [see comments]. Nature 385:74074-74083.
15. Yin DX, Zhu L, et al. 1996. Tetracycline-controlled gene expression system achieves high-level and quantitative control of gene expression. Anal Biochem 235:195-201.