DNA cleavage by type III restriction-modification enzyme EcoP15I is independent of spacer distance between two head to head oriented recognition sites

Journal of Molecular Biology

Volumn 312, Issue 4, 2001, Pages 687-698

  Mücke, Merlind ^a   Reich, Stefanie ^a   Möncke Buchner, Elisabeth ^a   Reuter, Monika ^a   Krüger, Detlev H ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

ATP hydrolysis; DNA foot printing; DNA restriction and modification; DNA translocation; EcoP15I restriction endonuclease

Indexed keywords

DNA; ENZYME; RESTRICTION MODIFICATION ENZYME ECOP 151; UNCLASSIFIED DRUG;

ARTICLE; DNA CLEAVAGE; DNA DETERMINATION; DNA FOOTPRINTING; DNA MODIFICATION; DNA RESTRICTION; DNA SEQUENCE; ESCHERICHIA COLI; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL;

EID: 0035965141     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4998     Document Type: Article

References (42)

1. Type III restriction enzymes need two inversely oriented recognition sites for DNA cleavage
2. Biology of DNA restriction
3. DNA restriction and modification systems
4. Type III restriction endonucleases translocate DNA in a reaction driven by recognition site-specific ATP hydrolysis
5. ATP hydrolysis is required for DNA cleavage by EcoP1 restriction enzyme
6. ATP dependent restriction enzymes
7. Model for how type I restriction enzymes select cleavage sites in DNA
8. Action at adistance along a DNA
9. Protein tracking-induced supercoiling of DNA: A tool to regulate DNA transactions in vivo?
10. The McrBC endonuclease translocates DNA in a reaction dependent on GTP hydrolysis
11. DNA restriction-modification genes of phage P1 and plasmid p15B
12. Purification and properties of a new restriction endonuclease from Haemophilus influenzae Rf
13. Identification of the recognition sequence of the M. StyLTI methyltransferase of Salmonella typhimrium LT7: An asymmetric site typical of type III enzymes
14. Subunit assembly and mode of DNA cleavage of the type III restriction endonucleases EcoP1I and EcoP15I
15. DNA restriction-modification enzymes of phage P1 and plasmid p15B: Subunit functions and structural homologies
16. EcoP15 methylates the second adenine in its recognition sequence
17. DNA recognition by the EcoP151 and EcoP1 modification methyltransferases
18. Host specificity of DNA produced by Escherichia coli. XII. The two restriction and modification systems of strain 15T
19. DNA recognition and cleavage by the EcoP15 restriction endonuclease
20. DNA Translocation blockage, a general mechanism of cleavage site selection by type I restriction enzymes
21. Mutual activation of two restriction endonucleases: Interaction of EcoP1 and EcoP15
22. DNA cleavage by the type IC restrictionmodification enzyme EcoR124II
23. A fluorescence based non-radioactive electrophoretic mobility shift assay
24. DNA looping
25. Steps in the reaction mechanism of the Escherichia coli plasmid P15-specific restriction endonuclease
26. Dependence of MerBC cleavage on distance between recognition elements
27. Functional analysis of conserved motifs in EcoP15I DNA methyltransferase
28. Binding of EcoP15I DNA methyltranferase to DNA reveals a large structural distortion within the recognition sequence
29. New restriction endonucleases from Flavobacterium okeanokoites (FokI) and Micrococcus luteus (MluI)
30. Structure of the multimodular endonuclease FokI bound to DNA
31. FokI dimerization is required for DNA cleavage
32. Structure of FokI has implications for DNA cleavage
33. Restriction by EcoKI is enhanced by cooperative interactions between target sequences and is dependent on DEAD box motifs
34. Methyl-specific DNA binding by McrBC, a modification dependent restriction enzyme
35. Direct observation of DNA translocation and cleavage by the EcoKI endonuclease using atomic force microscopy
36. Sequence-specific DNA binding by EcoKI, a type IA DNA restriction enzyme
37. Protein-protein and protein-DNA interactions in the type I restriction endonuclease EcoR124I
38. Role of ATP in the cleavage mechanism of the EcoP15 restriction endonuclease
39. Interaction of EcoP15I DNA methyltransferase with oligonucleotides containing the asymmetric sequence 5′-CAG-CAG
40. Purification and properties of the P15 specific restriction endonuclease from Escherichia coli