Enzymatic labeling of a single chain variable fragment of an antibody with alkaline phosphatase by microbial transglutaminase

Biotechnology and Bioengineering

Volumn 86, Issue 4, 2004, Pages 399-404

  Takazawa, Takeshi ^a   Kamiya, Noriho ^b   Ueda, Hiroshi ^a   Nagamune, Teruyuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

ELISA; Hybrid protein; Posttranslational modification; Protein cross linking; Site specific labeling; Transglutaminase

Indexed keywords

ANTIBODIES; BACTERIA; BIOASSAY; DIMERS; ENZYMES; REACTION KINETICS;

CONJUGATES; ENZYMATIC LABELING; SINGLE CHAIN VARIABLES;

BIOTECHNOLOGY;

ALKALINE PHOSPHATASE; DIMER; EGG WHITE; HYBRID PROTEIN; MICROBIAL ENZYME; PEPTIDE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; RECOMBINANT PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

AMINO TERMINAL SEQUENCE; ANTIBODY LABELING; ANTIGEN BINDING; ARTICLE; CONJUGATE; CROSS LINKING; ENZYME ACTIVITY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PROCESSING; STREPTOMYCES; STREPTOMYCES MOBARAENSIS;

ALKALINE PHOSPHATASE; BACTERIAL PROTEINS; CROSS-LINKING REAGENTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; IMMUNOGLOBULIN VARIABLE REGION; PROTEIN ENGINEERING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; STREPTOMYCES; TRANSGLUTAMINASES;

BACTERIA (MICROORGANISMS); STREPTOMYCES; STREPTOMYCES MOBARAENSIS;

EID: 2442683103     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20019     Document Type: Article

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