Ultrasensitive profiling and sequencing of N-linked oligosaccharides using standard DNA-sequencing equipment

Glycobiology

Volumn 11, Issue 4, 2001, Pages 275-281

  Callewaert, Nico ^a   Geysens, Steven ^a   Molemans, Francis ^a   Contreras, Roland ^a  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

APTS; DNA sequencer; MALDI TOF MS; N glycan; 1 acid glycoprotein

Indexed keywords

ASPARAGINE LINKED OLIGOSACCHARIDE; DNA; GLYCOPROTEIN; SEPHADEX; SULFONIC ACID DERIVATIVE;

ANALYTIC METHOD; ARTICLE; BIOLOGY; BIOMEDICINE; CAPILLARY ELECTROPHORESIS; CARBOHYDRATE ANALYSIS; CHEMICAL PROCEDURES; CONTROLLED STUDY; DEGLYCOSYLATION; DERIVATIZATION; DNA SEQUENCE; EQUIPMENT; GLYCOSYLATION; LABORATORY; MASS SPECTROMETRY; METHODOLOGY; PRIORITY JOURNAL; REPRODUCIBILITY; RESEARCH; SAMPLE; SENSITIVITY AND SPECIFICITY; STANDARD; TECHNOLOGY;

CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT DYES; GLYCOPROTEINS; GLYCOSYLATION; MOLECULAR SEQUENCE DATA; OLIGOSACCHARIDES; POLYSACCHARIDES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0034948464     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/11.4.275     Document Type: Article

References (19)

1. Analysis of mono- and oligosaccharide isomers derivatized with 9- aminopyrene-1, 4, 6-trisulfonate by capillary electrophoresis with laser- induced fluorescence
2. On-target exoglycosidase digestions/MALDI-MS for determining the primary structures of carbohydrate chains
3. Acid-catalyzed reductive amination of aldoses with 8-aminopyrene-1, 3, 6- trisulfonate
4. A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles
5. Separation of 1-amino-pyrene-3, 6, 8-trisulfonate-labeled asparagine-linked fetuin glycans by capillary gel electrophoresis
6. High-resolution capillary gel electrophoresis of reducing oligosaccharides labelled with 1-aminopyrene-3, 6, 8-trisulfonate
7. Postcolumn derivatization for chromatographic analysis of carbohydrates
8. Size fractionation of oligosaccharides
9. Analysis of 2-aminobenzamide-labeled oligosaccharides by high-pH anion- exchange chromatography with fluorometric detection
10. Structural determination of N-linked carbohydrates by matrix-assisted laser desorption/ionization-mass spectrometry following enzymatic release within sodium dodecyl sulphate-polyacrylamide electrophoresis gels: Species-specific glycosylation of alpha1-acid glycoprotein
11. Sequencing of N-linked oligosaccharides directly from protein gels: In- gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography
12. Study of the enzymatic transformation of fluorescently labeled oligosaccharides in human epidermoid cells using capillary electrophoresis with laser-induced fluorescence detection
13. Ultrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis
14. Analysis of starch structure using fluorophore-assisted carbohydrate electrophoresis
15. Use of scavenger beads to remove excess labeling reagents from capillary zone electrophoresis samples
16. A high-throughput microscale method to release N-linked oligosaccharides from glycoproteins for matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis
17. Detection of carbohydrates in capillary electrophoresis
18. Rapid, sensitive sequencing of oligosaccharides from glycoproteins
19. A tabulated review of capillary electrophoresis of carbohydrates